A modified assay for measuring thymocyte co-stimulatory activity
The observation that murine thymocytes increase their proliferation to interleukin 1 (IL-1) in the presence of phytohemagglutinin (PHA) when pre-incubated with interleukin 2 (IL-2) allowed the introduction of a modified assay for the measurement of IL-1 or the search of thymocyte-inducing proliferat...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Fundação Oswaldo Cruz (FIOCRUZ)
1993-09-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761993000300011 |
| _version_ | 1797704663700602880 |
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| author | Sergio R. Dalmau Claudia S. Freitas Wilson Savino |
| author_facet | Sergio R. Dalmau Claudia S. Freitas Wilson Savino |
| author_sort | Sergio R. Dalmau |
| collection | DOAJ |
| description | The observation that murine thymocytes increase their proliferation to interleukin 1 (IL-1) in the presence of phytohemagglutinin (PHA) when pre-incubated with interleukin 2 (IL-2) allowed the introduction of a modified assay for the measurement of IL-1 or the search of thymocyte-inducing proliferative activities in biological samples. Pre-incubation of thymocytes for 24 hr with 50 u/ml IL-2, followed by washings, elicited their maximal response to IL-1 in the usual lymphocyte activating factor (LAF) assay. This suggests that sequential events lead to thymocyte activation. The responsiveness is three to five fold greater than, and the total time of assay is the same as that of the LAF assay. Interestingly, pre-incubation with IL-2 renders thymocytes more sensitive than responsive to crude monocyte conditioned media. The use of the MTT colorimetric method for the assessment of thymocyte proliferation, and of the lectin jacalin as a co-mitogen are suggested as alternatives to be used in co-stimulatory assays. |
| first_indexed | 2024-03-12T05:23:48Z |
| format | Article |
| id | doaj.art-73adfdd15feb46f090fbb922ba60f627 |
| institution | Directory Open Access Journal |
| issn | 0074-0276 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T05:23:48Z |
| publishDate | 1993-09-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-73adfdd15feb46f090fbb922ba60f6272023-09-03T07:27:16ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz0074-02761678-80601993-09-0188341942510.1590/S0074-02761993000300011A modified assay for measuring thymocyte co-stimulatory activitySergio R. DalmauClaudia S. FreitasWilson SavinoThe observation that murine thymocytes increase their proliferation to interleukin 1 (IL-1) in the presence of phytohemagglutinin (PHA) when pre-incubated with interleukin 2 (IL-2) allowed the introduction of a modified assay for the measurement of IL-1 or the search of thymocyte-inducing proliferative activities in biological samples. Pre-incubation of thymocytes for 24 hr with 50 u/ml IL-2, followed by washings, elicited their maximal response to IL-1 in the usual lymphocyte activating factor (LAF) assay. This suggests that sequential events lead to thymocyte activation. The responsiveness is three to five fold greater than, and the total time of assay is the same as that of the LAF assay. Interestingly, pre-incubation with IL-2 renders thymocytes more sensitive than responsive to crude monocyte conditioned media. The use of the MTT colorimetric method for the assessment of thymocyte proliferation, and of the lectin jacalin as a co-mitogen are suggested as alternatives to be used in co-stimulatory assays.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761993000300011thymocyteco-stimulatory assaysinterleukin 1interleukin 2MTTJacalin |
| spellingShingle | Sergio R. Dalmau Claudia S. Freitas Wilson Savino A modified assay for measuring thymocyte co-stimulatory activity Memorias do Instituto Oswaldo Cruz thymocyte co-stimulatory assays interleukin 1 interleukin 2 MTT Jacalin |
| title | A modified assay for measuring thymocyte co-stimulatory activity |
| title_full | A modified assay for measuring thymocyte co-stimulatory activity |
| title_fullStr | A modified assay for measuring thymocyte co-stimulatory activity |
| title_full_unstemmed | A modified assay for measuring thymocyte co-stimulatory activity |
| title_short | A modified assay for measuring thymocyte co-stimulatory activity |
| title_sort | modified assay for measuring thymocyte co stimulatory activity |
| topic | thymocyte co-stimulatory assays interleukin 1 interleukin 2 MTT Jacalin |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761993000300011 |
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