Morphology of bovine corneal endothelial cells obtained with alizarin red and optical microscopy
The aim of this study was to determine the endothelial cell morphology in the different regions of healthy bovine corneal endothelium using optical microscopy. Twenty eyeballs from 10 male Brangus cattle, aged 24 months, were studied. The corneal endothelium was stained with the vital dye alizarin...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Universidade Federal de Goiás
2024-03-01
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| Series: | Ciência Animal Brasileira |
| Online Access: | https://revistas.ufg.br/vet/article/view/77041 |
| _version_ | 1797246862813560832 |
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| author | Natália Méndez Mariane Gallicchio Azevedo Luísa Soares Cargnin Maiara Poersch Seibel Alessandra Fernandez da Silva Maria Eduarda Mattos Franceschini Rafaela Silva Rocha João Antonio Tadeu Pigatto |
| author_facet | Natália Méndez Mariane Gallicchio Azevedo Luísa Soares Cargnin Maiara Poersch Seibel Alessandra Fernandez da Silva Maria Eduarda Mattos Franceschini Rafaela Silva Rocha João Antonio Tadeu Pigatto |
| author_sort | Natália Méndez |
| collection | DOAJ |
| description |
The aim of this study was to determine the endothelial cell morphology in the different regions of healthy bovine corneal endothelium using optical microscopy. Twenty eyeballs from 10 male Brangus cattle, aged 24 months, were studied. The corneal endothelium was stained with the vital dye alizarin red and then examined with an optical microscope and photographed. Thirty endothelial cells from each corneal region were included in the analysis. Endothelial cell morphology was analysed in the central, superior, inferior, lateral and medial regions of the cornea. Comparisons between regions were performed using repeated measures analysis of variance (ANOVA). Differences were considered statistically significant at P < 0.05. Normal endothelial cells were mainly hexagonal (83.7%), pentagonal (7.45%) and heptagonal (8.8%), with a minimal number of cells of other shapes present. No statistical differences were observed in the endothelial cell morphology when comparing different regions of the cornea. Regarding endothelial cell morphology, there were no differences between the corneal regions.
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| first_indexed | 2024-04-24T19:49:33Z |
| format | Article |
| id | doaj.art-73b0265f7cbb4cacacd9ef19bf5c9afe |
| institution | Directory Open Access Journal |
| issn | 1518-2797 1809-6891 |
| language | English |
| last_indexed | 2024-04-24T19:49:33Z |
| publishDate | 2024-03-01 |
| publisher | Universidade Federal de Goiás |
| record_format | Article |
| series | Ciência Animal Brasileira |
| spelling | doaj.art-73b0265f7cbb4cacacd9ef19bf5c9afe2024-03-24T23:59:20ZengUniversidade Federal de GoiásCiência Animal Brasileira1518-27971809-68912024-03-0125Morphology of bovine corneal endothelial cells obtained with alizarin red and optical microscopyNatália Méndez0Mariane Gallicchio Azevedo 1Luísa Soares Cargnin 2Maiara Poersch Seibel3Alessandra Fernandez da Silva4Maria Eduarda Mattos Franceschini 5Rafaela Silva Rocha 6João Antonio Tadeu Pigatto7Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, Brazil Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, Brazil Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, Brazil Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, Brazil Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, Brazil Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, Brazil Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, Brazil Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, Brazil The aim of this study was to determine the endothelial cell morphology in the different regions of healthy bovine corneal endothelium using optical microscopy. Twenty eyeballs from 10 male Brangus cattle, aged 24 months, were studied. The corneal endothelium was stained with the vital dye alizarin red and then examined with an optical microscope and photographed. Thirty endothelial cells from each corneal region were included in the analysis. Endothelial cell morphology was analysed in the central, superior, inferior, lateral and medial regions of the cornea. Comparisons between regions were performed using repeated measures analysis of variance (ANOVA). Differences were considered statistically significant at P < 0.05. Normal endothelial cells were mainly hexagonal (83.7%), pentagonal (7.45%) and heptagonal (8.8%), with a minimal number of cells of other shapes present. No statistical differences were observed in the endothelial cell morphology when comparing different regions of the cornea. Regarding endothelial cell morphology, there were no differences between the corneal regions. https://revistas.ufg.br/vet/article/view/77041 |
| spellingShingle | Natália Méndez Mariane Gallicchio Azevedo Luísa Soares Cargnin Maiara Poersch Seibel Alessandra Fernandez da Silva Maria Eduarda Mattos Franceschini Rafaela Silva Rocha João Antonio Tadeu Pigatto Morphology of bovine corneal endothelial cells obtained with alizarin red and optical microscopy Ciência Animal Brasileira |
| title | Morphology of bovine corneal endothelial cells obtained with alizarin red and optical microscopy |
| title_full | Morphology of bovine corneal endothelial cells obtained with alizarin red and optical microscopy |
| title_fullStr | Morphology of bovine corneal endothelial cells obtained with alizarin red and optical microscopy |
| title_full_unstemmed | Morphology of bovine corneal endothelial cells obtained with alizarin red and optical microscopy |
| title_short | Morphology of bovine corneal endothelial cells obtained with alizarin red and optical microscopy |
| title_sort | morphology of bovine corneal endothelial cells obtained with alizarin red and optical microscopy |
| url | https://revistas.ufg.br/vet/article/view/77041 |
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