Energetically exploiting lignocellulose-rich residues in anaerobic digestion technologies: from bioreactors to proteogenomics
Abstract The biogas produced through anaerobic digestion (AD) of renewable feedstocks is one of the promising alternatives to replace fossil-derived energy. Even though lignocellulosic biomass is the most abundant biomass on earth, only a small fraction is being used towards resources recovery, leav...
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| Format: | Article |
| Language: | English |
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BMC
2023-11-01
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| Series: | Biotechnology for Biofuels and Bioproducts |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13068-023-02432-x |
| _version_ | 1827604104132689920 |
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| author | Jan Struckmann Poulsen Williane Vieira Macêdo Torben Bonde Jeppe Lund Nielsen |
| author_facet | Jan Struckmann Poulsen Williane Vieira Macêdo Torben Bonde Jeppe Lund Nielsen |
| author_sort | Jan Struckmann Poulsen |
| collection | DOAJ |
| description | Abstract The biogas produced through anaerobic digestion (AD) of renewable feedstocks is one of the promising alternatives to replace fossil-derived energy. Even though lignocellulosic biomass is the most abundant biomass on earth, only a small fraction is being used towards resources recovery, leaving a great potential unexploited. In this study, the combination of state-of-art genomic techniques and engineered systems were used to further advance the knowledge on biogas production from lignocellulosic-rich residues and the microbiome involved in the anaerobic digestion hereof. A long-term adapted anaerobic microbiome capable of degrading wheat straw as the sole substrate was investigated using protein stable isotope probing (protein-SIP). The results indicated that a diverse microbial community, primarily composed of Firmicutes and Methanogens, played crucial roles in cellulose degradation and methane production. Notably, Defluviitoga tunisiensis, Syntrophothermus lipocalidus, and Pelobacter carbinolicus were identified as direct metabolizers of cellulose, while Dehalobacterium assimilated labelled carbon through cross-feeding. This study provides direct evidence of primary cellulose degraders and sheds light on their genomic composition. By harnessing the potential of lignocellulosic biomass and understanding the microbial communities involved, we can promote sustainable biogas production, contributing to energy security and environmental preservation. |
| first_indexed | 2024-03-09T05:54:19Z |
| format | Article |
| id | doaj.art-73b0d3e7b0bf429499a3babad068e18c |
| institution | Directory Open Access Journal |
| issn | 2731-3654 |
| language | English |
| last_indexed | 2024-03-09T05:54:19Z |
| publishDate | 2023-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Biotechnology for Biofuels and Bioproducts |
| spelling | doaj.art-73b0d3e7b0bf429499a3babad068e18c2023-12-03T12:15:11ZengBMCBiotechnology for Biofuels and Bioproducts2731-36542023-11-0116111110.1186/s13068-023-02432-xEnergetically exploiting lignocellulose-rich residues in anaerobic digestion technologies: from bioreactors to proteogenomicsJan Struckmann Poulsen0Williane Vieira Macêdo1Torben Bonde2Jeppe Lund Nielsen3Department of Chemistry and Bioscience, Aalborg UniversityDepartment of Chemistry and Bioscience, Aalborg UniversityBiofuel Technology A/SDepartment of Chemistry and Bioscience, Aalborg UniversityAbstract The biogas produced through anaerobic digestion (AD) of renewable feedstocks is one of the promising alternatives to replace fossil-derived energy. Even though lignocellulosic biomass is the most abundant biomass on earth, only a small fraction is being used towards resources recovery, leaving a great potential unexploited. In this study, the combination of state-of-art genomic techniques and engineered systems were used to further advance the knowledge on biogas production from lignocellulosic-rich residues and the microbiome involved in the anaerobic digestion hereof. A long-term adapted anaerobic microbiome capable of degrading wheat straw as the sole substrate was investigated using protein stable isotope probing (protein-SIP). The results indicated that a diverse microbial community, primarily composed of Firmicutes and Methanogens, played crucial roles in cellulose degradation and methane production. Notably, Defluviitoga tunisiensis, Syntrophothermus lipocalidus, and Pelobacter carbinolicus were identified as direct metabolizers of cellulose, while Dehalobacterium assimilated labelled carbon through cross-feeding. This study provides direct evidence of primary cellulose degraders and sheds light on their genomic composition. By harnessing the potential of lignocellulosic biomass and understanding the microbial communities involved, we can promote sustainable biogas production, contributing to energy security and environmental preservation.https://doi.org/10.1186/s13068-023-02432-xLignocelluloseAnaerobic digestionProtein stable isotope probingMetaproteomics |
| spellingShingle | Jan Struckmann Poulsen Williane Vieira Macêdo Torben Bonde Jeppe Lund Nielsen Energetically exploiting lignocellulose-rich residues in anaerobic digestion technologies: from bioreactors to proteogenomics Biotechnology for Biofuels and Bioproducts Lignocellulose Anaerobic digestion Protein stable isotope probing Metaproteomics |
| title | Energetically exploiting lignocellulose-rich residues in anaerobic digestion technologies: from bioreactors to proteogenomics |
| title_full | Energetically exploiting lignocellulose-rich residues in anaerobic digestion technologies: from bioreactors to proteogenomics |
| title_fullStr | Energetically exploiting lignocellulose-rich residues in anaerobic digestion technologies: from bioreactors to proteogenomics |
| title_full_unstemmed | Energetically exploiting lignocellulose-rich residues in anaerobic digestion technologies: from bioreactors to proteogenomics |
| title_short | Energetically exploiting lignocellulose-rich residues in anaerobic digestion technologies: from bioreactors to proteogenomics |
| title_sort | energetically exploiting lignocellulose rich residues in anaerobic digestion technologies from bioreactors to proteogenomics |
| topic | Lignocellulose Anaerobic digestion Protein stable isotope probing Metaproteomics |
| url | https://doi.org/10.1186/s13068-023-02432-x |
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