Phytochemical screening and in vitro evaluation of antioxidant and antibacterial activities of Teucrium trifidum crude extracts
The objective of the investigation was to identify biologically active polyphenols and to determine the antioxidant and antimicrobial capacity of Teucrium trifidum extracted with different organic solvents (acetone, ethanol and methanol) and distilled water. The results of the study revealed varying...
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| Format: | Article |
| Language: | English |
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Elsevier
2020-09-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844020312391 |
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| author | Irene Rumbidzai Mazhangara Emrobowansan Monday Idamokoro Eliton Chivandi Anthony Jide Afolayan |
| author_facet | Irene Rumbidzai Mazhangara Emrobowansan Monday Idamokoro Eliton Chivandi Anthony Jide Afolayan |
| author_sort | Irene Rumbidzai Mazhangara |
| collection | DOAJ |
| description | The objective of the investigation was to identify biologically active polyphenols and to determine the antioxidant and antimicrobial capacity of Teucrium trifidum extracted with different organic solvents (acetone, ethanol and methanol) and distilled water. The results of the study revealed varying levels of polyphenols in the different solvent extracts. Condensed tannin, flavonoid and total phenolic content ranged from (77.339 ± 1.068) to (99.395 ± 1.490) mg CE/g; (3.398 ± 0.2410) to (53.253 ± 0.638) mg QE/g; (14.1087 ± 0.0915) to (21.7977 ± 0.0279) mg GAE/g, respectively. The extracts demonstrated high antioxidant activity in 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH), nitric oxide (NO) and total antioxidant capacity (TAC) assays which were comparable to rutin and butylated hydroxytoluene (BHT) and increased with increasing concentrations of polyphenols extract (P < 0.05). The agar dilution assay of acetone, ethanol and methanol extracts revealed an appreciable broad-spectrum activity against tested pathogenic bacteria. The findings of this study provide evidence that T. trifidum can be used as a natural source of antioxidant and antimicrobial components. |
| first_indexed | 2024-12-13T04:50:25Z |
| format | Article |
| id | doaj.art-73b400a61df14082a6be41fed78fdfa8 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-12-13T04:50:25Z |
| publishDate | 2020-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-73b400a61df14082a6be41fed78fdfa82022-12-21T23:59:01ZengElsevierHeliyon2405-84402020-09-0169e04395Phytochemical screening and in vitro evaluation of antioxidant and antibacterial activities of Teucrium trifidum crude extractsIrene Rumbidzai Mazhangara0Emrobowansan Monday Idamokoro1Eliton Chivandi2Anthony Jide Afolayan3Department of Livestock and Pasture Science, University of Fort Hare, Private Bag X1314, Alice 5700, South Africa; Corresponding author.Department of Livestock and Pasture Science, University of Fort Hare, Private Bag X1314, Alice 5700, South Africa; MPED Research Center, Department of Botany, University of Fort Hare, Private Bag X1314, Alice 5700, South AfricaSchool of Physiology, University of Witwatersrand, 7 York Road, Parktown, Johannesburg 2193, South AfricaMPED Research Center, Department of Botany, University of Fort Hare, Private Bag X1314, Alice 5700, South AfricaThe objective of the investigation was to identify biologically active polyphenols and to determine the antioxidant and antimicrobial capacity of Teucrium trifidum extracted with different organic solvents (acetone, ethanol and methanol) and distilled water. The results of the study revealed varying levels of polyphenols in the different solvent extracts. Condensed tannin, flavonoid and total phenolic content ranged from (77.339 ± 1.068) to (99.395 ± 1.490) mg CE/g; (3.398 ± 0.2410) to (53.253 ± 0.638) mg QE/g; (14.1087 ± 0.0915) to (21.7977 ± 0.0279) mg GAE/g, respectively. The extracts demonstrated high antioxidant activity in 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH), nitric oxide (NO) and total antioxidant capacity (TAC) assays which were comparable to rutin and butylated hydroxytoluene (BHT) and increased with increasing concentrations of polyphenols extract (P < 0.05). The agar dilution assay of acetone, ethanol and methanol extracts revealed an appreciable broad-spectrum activity against tested pathogenic bacteria. The findings of this study provide evidence that T. trifidum can be used as a natural source of antioxidant and antimicrobial components.http://www.sciencedirect.com/science/article/pii/S2405844020312391Food safetyFood scienceFood technologyNutritionAntioxidant activityAntimicrobial activity |
| spellingShingle | Irene Rumbidzai Mazhangara Emrobowansan Monday Idamokoro Eliton Chivandi Anthony Jide Afolayan Phytochemical screening and in vitro evaluation of antioxidant and antibacterial activities of Teucrium trifidum crude extracts Heliyon Food safety Food science Food technology Nutrition Antioxidant activity Antimicrobial activity |
| title | Phytochemical screening and in vitro evaluation of antioxidant and antibacterial activities of Teucrium trifidum crude extracts |
| title_full | Phytochemical screening and in vitro evaluation of antioxidant and antibacterial activities of Teucrium trifidum crude extracts |
| title_fullStr | Phytochemical screening and in vitro evaluation of antioxidant and antibacterial activities of Teucrium trifidum crude extracts |
| title_full_unstemmed | Phytochemical screening and in vitro evaluation of antioxidant and antibacterial activities of Teucrium trifidum crude extracts |
| title_short | Phytochemical screening and in vitro evaluation of antioxidant and antibacterial activities of Teucrium trifidum crude extracts |
| title_sort | phytochemical screening and in vitro evaluation of antioxidant and antibacterial activities of teucrium trifidum crude extracts |
| topic | Food safety Food science Food technology Nutrition Antioxidant activity Antimicrobial activity |
| url | http://www.sciencedirect.com/science/article/pii/S2405844020312391 |
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