Competition Between Chemolithotrophic Acetogenesis and Hydrogenotrophic Methanogenesis for Exogenous H2/CO2 in Anaerobically Digested Sludge: Impact of Temperature
Anaerobic digestion is a widely applied technology for sewage sludge treatment. Hydrogen and CO2 are important degradation products, which serve as substrates for both hydrogenotrophic methanogenesis and chemolithotrophic acetogenesis. In order to understand the competition between these processes f...
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Frontiers Media S.A.
2019-10-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2019.02418/full |
| _version_ | 1829118594440495104 |
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| author | Bo Fu Bo Fu Bo Fu Xin Jin Ralf Conrad Hongbo Liu Hongbo Liu He Liu He Liu |
| author_facet | Bo Fu Bo Fu Bo Fu Xin Jin Ralf Conrad Hongbo Liu Hongbo Liu He Liu He Liu |
| author_sort | Bo Fu |
| collection | DOAJ |
| description | Anaerobic digestion is a widely applied technology for sewage sludge treatment. Hydrogen and CO2 are important degradation products, which serve as substrates for both hydrogenotrophic methanogenesis and chemolithotrophic acetogenesis. In order to understand the competition between these processes for H2/CO2, sludge samples were incubated under H2/CO2 headspace at different temperatures, and analyzed with respect to turnover of H2, CO2, CH4 and acetate including their δ13C values. At 15°C, 13C-depleted acetate (δ13C of −41 to −43‰) and transient acetate accumulation were observed under H2/CO2, and CH4 accumulated with δ13C values increasing from −53 to −33‰. The copy numbers of the fhs gene, which is characteristic for acetogenic bacteria, were at 15°C one order of magnitude higher in the H2/CO2 incubations than the N2 control. At 30°C, however, acetate did not accumulate in the H2/CO2 incubation and the δ13C of CH4 was very low (−100 to −77‰). At 50°C, isotopically enriched acetate was transiently formed and subsequently consumed followed by the production of 13C-depleted CH4. Collectively, the results indicate a high contribution of chemolithotrophic acetogenesis to H2/CO2 utilization at 15°C and 50°C, while H2/CO2 was mainly consumed by hydrogenotrophic methanogenesis at 30°C. Fermentative production and methanogenic consumption of acetate were active at 50°C. |
| first_indexed | 2024-12-13T10:40:28Z |
| format | Article |
| id | doaj.art-73b75710db30413aa262d7ef9a90fe24 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-13T10:40:28Z |
| publishDate | 2019-10-01 |
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| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-73b75710db30413aa262d7ef9a90fe242022-12-21T23:50:32ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2019-10-011010.3389/fmicb.2019.02418484483Competition Between Chemolithotrophic Acetogenesis and Hydrogenotrophic Methanogenesis for Exogenous H2/CO2 in Anaerobically Digested Sludge: Impact of TemperatureBo Fu0Bo Fu1Bo Fu2Xin Jin3Ralf Conrad4Hongbo Liu5Hongbo Liu6He Liu7He Liu8Jiangsu Key Laboratory of Anaerobic Biotechnology, School of Environmental and Civil Engineering, Jiangnan University, Wuxi, ChinaDepartment of Biogeochemistry, Max Planck Institute for Terrestrial Microbiology, Marburg, GermanyJiangsu Collaborative Innovation Center of Technology and Material of Water Treatment, Suzhou, ChinaJiangsu Key Laboratory of Anaerobic Biotechnology, School of Environmental and Civil Engineering, Jiangnan University, Wuxi, ChinaDepartment of Biogeochemistry, Max Planck Institute for Terrestrial Microbiology, Marburg, GermanyJiangsu Key Laboratory of Anaerobic Biotechnology, School of Environmental and Civil Engineering, Jiangnan University, Wuxi, ChinaJiangsu Collaborative Innovation Center of Technology and Material of Water Treatment, Suzhou, ChinaJiangsu Key Laboratory of Anaerobic Biotechnology, School of Environmental and Civil Engineering, Jiangnan University, Wuxi, ChinaJiangsu Collaborative Innovation Center of Technology and Material of Water Treatment, Suzhou, ChinaAnaerobic digestion is a widely applied technology for sewage sludge treatment. Hydrogen and CO2 are important degradation products, which serve as substrates for both hydrogenotrophic methanogenesis and chemolithotrophic acetogenesis. In order to understand the competition between these processes for H2/CO2, sludge samples were incubated under H2/CO2 headspace at different temperatures, and analyzed with respect to turnover of H2, CO2, CH4 and acetate including their δ13C values. At 15°C, 13C-depleted acetate (δ13C of −41 to −43‰) and transient acetate accumulation were observed under H2/CO2, and CH4 accumulated with δ13C values increasing from −53 to −33‰. The copy numbers of the fhs gene, which is characteristic for acetogenic bacteria, were at 15°C one order of magnitude higher in the H2/CO2 incubations than the N2 control. At 30°C, however, acetate did not accumulate in the H2/CO2 incubation and the δ13C of CH4 was very low (−100 to −77‰). At 50°C, isotopically enriched acetate was transiently formed and subsequently consumed followed by the production of 13C-depleted CH4. Collectively, the results indicate a high contribution of chemolithotrophic acetogenesis to H2/CO2 utilization at 15°C and 50°C, while H2/CO2 was mainly consumed by hydrogenotrophic methanogenesis at 30°C. Fermentative production and methanogenic consumption of acetate were active at 50°C.https://www.frontiersin.org/article/10.3389/fmicb.2019.02418/fullmethanogenesisacetogenesiscarbon isotopetemperatureH2/CO2 utilization |
| spellingShingle | Bo Fu Bo Fu Bo Fu Xin Jin Ralf Conrad Hongbo Liu Hongbo Liu He Liu He Liu Competition Between Chemolithotrophic Acetogenesis and Hydrogenotrophic Methanogenesis for Exogenous H2/CO2 in Anaerobically Digested Sludge: Impact of Temperature Frontiers in Microbiology methanogenesis acetogenesis carbon isotope temperature H2/CO2 utilization |
| title | Competition Between Chemolithotrophic Acetogenesis and Hydrogenotrophic Methanogenesis for Exogenous H2/CO2 in Anaerobically Digested Sludge: Impact of Temperature |
| title_full | Competition Between Chemolithotrophic Acetogenesis and Hydrogenotrophic Methanogenesis for Exogenous H2/CO2 in Anaerobically Digested Sludge: Impact of Temperature |
| title_fullStr | Competition Between Chemolithotrophic Acetogenesis and Hydrogenotrophic Methanogenesis for Exogenous H2/CO2 in Anaerobically Digested Sludge: Impact of Temperature |
| title_full_unstemmed | Competition Between Chemolithotrophic Acetogenesis and Hydrogenotrophic Methanogenesis for Exogenous H2/CO2 in Anaerobically Digested Sludge: Impact of Temperature |
| title_short | Competition Between Chemolithotrophic Acetogenesis and Hydrogenotrophic Methanogenesis for Exogenous H2/CO2 in Anaerobically Digested Sludge: Impact of Temperature |
| title_sort | competition between chemolithotrophic acetogenesis and hydrogenotrophic methanogenesis for exogenous h2 co2 in anaerobically digested sludge impact of temperature |
| topic | methanogenesis acetogenesis carbon isotope temperature H2/CO2 utilization |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2019.02418/full |
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