Analysis and Validation of Hub Genes in Blood Monocytes of Postmenopausal Osteoporosis Patients
Osteoporosis is a common systemic bone disease caused by the imbalance between osteogenic activity and osteoclastic activity. Aged women are at higher risk of osteoporosis, partly because of estrogen deficiency. However, the underlying mechanism of how estrogen deficiency affects osteoclast activity...
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Frontiers Media S.A.
2022-01-01
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| Series: | Frontiers in Endocrinology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fendo.2021.815245/full |
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| author | Yi-Xuan Deng Yi-Xuan Deng Wen-Ge He Wen-Ge He Wen-Ge He Hai-Jun Cai Hai-Jun Cai Jin-Hai Jiang Jin-Hai Jiang Yuan-Yuan Yang Yuan-Yuan Yang Yan-Rong Dan Yan-Rong Dan Hong-Hong Luo Hong-Hong Luo Yu Du Liang Chen Liang Chen Bai-Cheng He Bai-Cheng He |
| author_facet | Yi-Xuan Deng Yi-Xuan Deng Wen-Ge He Wen-Ge He Wen-Ge He Hai-Jun Cai Hai-Jun Cai Jin-Hai Jiang Jin-Hai Jiang Yuan-Yuan Yang Yuan-Yuan Yang Yan-Rong Dan Yan-Rong Dan Hong-Hong Luo Hong-Hong Luo Yu Du Liang Chen Liang Chen Bai-Cheng He Bai-Cheng He |
| author_sort | Yi-Xuan Deng |
| collection | DOAJ |
| description | Osteoporosis is a common systemic bone disease caused by the imbalance between osteogenic activity and osteoclastic activity. Aged women are at higher risk of osteoporosis, partly because of estrogen deficiency. However, the underlying mechanism of how estrogen deficiency affects osteoclast activity has not yet been well elucidated. In this study, GSE2208 and GSE56815 datasets were downloaded from GEO database with 25 PreH BMD women and 25 PostL BMD women in total. The RRA algorithm determined 38 downregulated DEGs and 30 upregulated DEGs. Through GO analysis, we found that downregulated DEGs were mainly enriched in myeloid cell differentiation, cytokine-related functions while upregulated DEGs enriched in immune-related biological processes; pathways like Notch signaling and MAPK activation were found in KEGG/Rectome pathway database; a PPI network which contains 66 nodes and 91 edges was constructed and three Modules were obtained by Mcode; Correlation analysis helped us to find highly correlated genes in each module. Moreover, three hub genes FOS, PTPN6, and CTSD were captured by Cytohubba. Finally, the hub genes were further confirmed in blood monocytes of ovariectomy (OVX) rats by real-time PCR assay. In conclusion, the integrative bioinformatics analysis and real-time PCR analysis were utilized to offer fresh light into the role of monocytes in premenopausal osteoporosis and identified FOS, PTPN6, and CTSD as potential biomarkers for postmenopausal osteoporosis. |
| first_indexed | 2024-12-18T05:31:14Z |
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| institution | Directory Open Access Journal |
| issn | 1664-2392 |
| language | English |
| last_indexed | 2024-12-18T05:31:14Z |
| publishDate | 2022-01-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Endocrinology |
| spelling | doaj.art-73bc4ef8ab754fa69160813ed881375b2022-12-21T21:19:26ZengFrontiers Media S.A.Frontiers in Endocrinology1664-23922022-01-011210.3389/fendo.2021.815245815245Analysis and Validation of Hub Genes in Blood Monocytes of Postmenopausal Osteoporosis PatientsYi-Xuan Deng0Yi-Xuan Deng1Wen-Ge He2Wen-Ge He3Wen-Ge He4Hai-Jun Cai5Hai-Jun Cai6Jin-Hai Jiang7Jin-Hai Jiang8Yuan-Yuan Yang9Yuan-Yuan Yang10Yan-Rong Dan11Yan-Rong Dan12Hong-Hong Luo13Hong-Hong Luo14Yu Du15Liang Chen16Liang Chen17Bai-Cheng He18Bai-Cheng He19Department of Pharmacology, School of Pharmacy, Chongqing Medical University, Chongqing, ChinaKey Laboratory of Biochemistry and Molecular Pharmacology of Chongqing, Chongqing Medical University, Chongqing, ChinaKey Laboratory of Biochemistry and Molecular Pharmacology of Chongqing, Chongqing Medical University, Chongqing, ChinaDepartment of Orthopaedics, The Second Affiliated Hospital, Chongqing Medical University, Chongqing, ChinaDepartment of Bone and Soft Tissue Oncology, Chongqing University Cancer Hospital, Chongqing, ChinaDepartment of Pharmacology, School of Pharmacy, Chongqing Medical University, Chongqing, ChinaKey Laboratory of Biochemistry and Molecular Pharmacology of Chongqing, Chongqing Medical University, Chongqing, ChinaDepartment of Pharmacology, School of Pharmacy, Chongqing Medical University, Chongqing, ChinaKey Laboratory of Biochemistry and Molecular Pharmacology of Chongqing, Chongqing Medical University, Chongqing, ChinaDepartment of Pharmacology, School of Pharmacy, Chongqing Medical University, Chongqing, ChinaKey Laboratory of Biochemistry and Molecular Pharmacology of Chongqing, Chongqing Medical University, Chongqing, ChinaDepartment of Pharmacology, School of Pharmacy, Chongqing Medical University, Chongqing, ChinaKey Laboratory of Biochemistry and Molecular Pharmacology of Chongqing, Chongqing Medical University, Chongqing, ChinaDepartment of Pharmacology, School of Pharmacy, Chongqing Medical University, Chongqing, ChinaKey Laboratory of Biochemistry and Molecular Pharmacology of Chongqing, Chongqing Medical University, Chongqing, ChinaDepartment of Orthopaedics, The Second Affiliated Hospital, Chongqing Medical University, Chongqing, ChinaDepartment of Orthopaedics, The Second Affiliated Hospital, Chongqing Medical University, Chongqing, ChinaDepartment of Bone and Soft Tissue Oncology, Chongqing University Cancer Hospital, Chongqing, ChinaDepartment of Pharmacology, School of Pharmacy, Chongqing Medical University, Chongqing, ChinaKey Laboratory of Biochemistry and Molecular Pharmacology of Chongqing, Chongqing Medical University, Chongqing, ChinaOsteoporosis is a common systemic bone disease caused by the imbalance between osteogenic activity and osteoclastic activity. Aged women are at higher risk of osteoporosis, partly because of estrogen deficiency. However, the underlying mechanism of how estrogen deficiency affects osteoclast activity has not yet been well elucidated. In this study, GSE2208 and GSE56815 datasets were downloaded from GEO database with 25 PreH BMD women and 25 PostL BMD women in total. The RRA algorithm determined 38 downregulated DEGs and 30 upregulated DEGs. Through GO analysis, we found that downregulated DEGs were mainly enriched in myeloid cell differentiation, cytokine-related functions while upregulated DEGs enriched in immune-related biological processes; pathways like Notch signaling and MAPK activation were found in KEGG/Rectome pathway database; a PPI network which contains 66 nodes and 91 edges was constructed and three Modules were obtained by Mcode; Correlation analysis helped us to find highly correlated genes in each module. Moreover, three hub genes FOS, PTPN6, and CTSD were captured by Cytohubba. Finally, the hub genes were further confirmed in blood monocytes of ovariectomy (OVX) rats by real-time PCR assay. In conclusion, the integrative bioinformatics analysis and real-time PCR analysis were utilized to offer fresh light into the role of monocytes in premenopausal osteoporosis and identified FOS, PTPN6, and CTSD as potential biomarkers for postmenopausal osteoporosis.https://www.frontiersin.org/articles/10.3389/fendo.2021.815245/fullpostmenopausal osteoporosismicroarrayenrichment analysishub genesOVX |
| spellingShingle | Yi-Xuan Deng Yi-Xuan Deng Wen-Ge He Wen-Ge He Wen-Ge He Hai-Jun Cai Hai-Jun Cai Jin-Hai Jiang Jin-Hai Jiang Yuan-Yuan Yang Yuan-Yuan Yang Yan-Rong Dan Yan-Rong Dan Hong-Hong Luo Hong-Hong Luo Yu Du Liang Chen Liang Chen Bai-Cheng He Bai-Cheng He Analysis and Validation of Hub Genes in Blood Monocytes of Postmenopausal Osteoporosis Patients Frontiers in Endocrinology postmenopausal osteoporosis microarray enrichment analysis hub genes OVX |
| title | Analysis and Validation of Hub Genes in Blood Monocytes of Postmenopausal Osteoporosis Patients |
| title_full | Analysis and Validation of Hub Genes in Blood Monocytes of Postmenopausal Osteoporosis Patients |
| title_fullStr | Analysis and Validation of Hub Genes in Blood Monocytes of Postmenopausal Osteoporosis Patients |
| title_full_unstemmed | Analysis and Validation of Hub Genes in Blood Monocytes of Postmenopausal Osteoporosis Patients |
| title_short | Analysis and Validation of Hub Genes in Blood Monocytes of Postmenopausal Osteoporosis Patients |
| title_sort | analysis and validation of hub genes in blood monocytes of postmenopausal osteoporosis patients |
| topic | postmenopausal osteoporosis microarray enrichment analysis hub genes OVX |
| url | https://www.frontiersin.org/articles/10.3389/fendo.2021.815245/full |
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