Creation of combined stabilizing compositions to preserve the activity of gonadotropins in liquid form
The activity of dissolved enzyme preparations during storage decreases, what leads to the loss of their biological activity and, as a result, reduces the effectiveness of the drugs. Therefore, the development of compositions that are able to maintain high activity of the hormone in dissolved form du...
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| Format: | Article |
| Language: | English |
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Institute of Animal Biology NAAS
2022-10-01
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| Series: | Біологія тварин |
| Subjects: | |
| Online Access: | https://aminbiol.com.ua/index.php/archive/191-archive/bt-24-3-2022/2028-creation-of-combined-stabilizing-compositions-to-preserve-the-activity-of-gonadotropins-in-liquid-form |
| _version_ | 1797374780852142080 |
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| author | O. V. Shtapenko I. I. Gevkan V. Y. Syrvatka O. Y. Slyvchuk O. O. Korbetska S. B. Kornyat I. M. Yaremchuk |
| author_facet | O. V. Shtapenko I. I. Gevkan V. Y. Syrvatka O. Y. Slyvchuk O. O. Korbetska S. B. Kornyat I. M. Yaremchuk |
| author_sort | O. V. Shtapenko |
| collection | DOAJ |
| description | The activity of dissolved enzyme preparations during storage decreases, what leads to the loss of their biological activity and, as a result, reduces the effectiveness of the drugs. Therefore, the development of compositions that are able to maintain high activity of the hormone in dissolved form during long-term storage is relevant. The results of studies have shown that using sucrose as a stabilizing component for maintain gonadotropin activity is effective. It was found that during eight weeks of storage the best results on the preservation of gonadotropin activity during storage at 40°C were obtained in samples containing 75 mg/ml of sucrose compared to the sample of the control group. However, the highest gonadotropin activity was found when — 10 mg/ml L-lysine and 75 mg/ml sucrose were used as stabilizers. Studies of the dynamics of gonadotropin activity during long-term storage at 18–20°C showed that the addition of L-lysine and sucrose as stabilizing substances in the form of liposomal emulsion increases the preservation of chorionic hormone activity for 2 weeks of storage by 11.4% compared to similar composition pharmacological composition of the drug in aqueous form. |
| first_indexed | 2024-03-08T19:10:40Z |
| format | Article |
| id | doaj.art-73bd5bacd9c844da86d55c60cc196064 |
| institution | Directory Open Access Journal |
| issn | 1681-0015 2313-2191 |
| language | English |
| last_indexed | 2024-03-08T19:10:40Z |
| publishDate | 2022-10-01 |
| publisher | Institute of Animal Biology NAAS |
| record_format | Article |
| series | Біологія тварин |
| spelling | doaj.art-73bd5bacd9c844da86d55c60cc1960642023-12-27T14:08:53ZengInstitute of Animal Biology NAASБіологія тварин1681-00152313-21912022-10-01243141710.15407/animbiol24.03.014Creation of combined stabilizing compositions to preserve the activity of gonadotropins in liquid formO. V. Shtapenko0https://orcid.org/0000-0002-1192-8432I. I. Gevkan1https://orcid.org/0000-0002-5480-785XV. Y. Syrvatka2https://orcid.org/0000-0003-1326-6206O. Y. Slyvchuk3https://orcid.org/0000-0002-0229-5848O. O. Korbetska4https://orcid.org/0000-0002-0689-6835S. B. Kornyat5https://orcid.org/0000-0002-1430-7754I. M. Yaremchuk6https://orcid.org/0000-0001-8787-2132Institute of Animal Biology NAASInstitute of Animal Biology NAASIvan Franko National University of LvivInstitute of Animal Biology NAASInstitute of Animal Biology NAASInstitute of Animal Biology NAASInstitute of Animal Biology NAASThe activity of dissolved enzyme preparations during storage decreases, what leads to the loss of their biological activity and, as a result, reduces the effectiveness of the drugs. Therefore, the development of compositions that are able to maintain high activity of the hormone in dissolved form during long-term storage is relevant. The results of studies have shown that using sucrose as a stabilizing component for maintain gonadotropin activity is effective. It was found that during eight weeks of storage the best results on the preservation of gonadotropin activity during storage at 40°C were obtained in samples containing 75 mg/ml of sucrose compared to the sample of the control group. However, the highest gonadotropin activity was found when — 10 mg/ml L-lysine and 75 mg/ml sucrose were used as stabilizers. Studies of the dynamics of gonadotropin activity during long-term storage at 18–20°C showed that the addition of L-lysine and sucrose as stabilizing substances in the form of liposomal emulsion increases the preservation of chorionic hormone activity for 2 weeks of storage by 11.4% compared to similar composition pharmacological composition of the drug in aqueous form.https://aminbiol.com.ua/index.php/archive/191-archive/bt-24-3-2022/2028-creation-of-combined-stabilizing-compositions-to-preserve-the-activity-of-gonadotropins-in-liquid-formхоріонічний гормон людиниактивністьстабілізаціяцукрозаманітоллізинhuman chorionic hormoneactivitystabilizationsucrosemannitollysine |
| spellingShingle | O. V. Shtapenko I. I. Gevkan V. Y. Syrvatka O. Y. Slyvchuk O. O. Korbetska S. B. Kornyat I. M. Yaremchuk Creation of combined stabilizing compositions to preserve the activity of gonadotropins in liquid form Біологія тварин хоріонічний гормон людини активність стабілізація цукроза манітол лізин human chorionic hormone activity stabilization sucrose mannitol lysine |
| title | Creation of combined stabilizing compositions to preserve the activity of gonadotropins in liquid form |
| title_full | Creation of combined stabilizing compositions to preserve the activity of gonadotropins in liquid form |
| title_fullStr | Creation of combined stabilizing compositions to preserve the activity of gonadotropins in liquid form |
| title_full_unstemmed | Creation of combined stabilizing compositions to preserve the activity of gonadotropins in liquid form |
| title_short | Creation of combined stabilizing compositions to preserve the activity of gonadotropins in liquid form |
| title_sort | creation of combined stabilizing compositions to preserve the activity of gonadotropins in liquid form |
| topic | хоріонічний гормон людини активність стабілізація цукроза манітол лізин human chorionic hormone activity stabilization sucrose mannitol lysine |
| url | https://aminbiol.com.ua/index.php/archive/191-archive/bt-24-3-2022/2028-creation-of-combined-stabilizing-compositions-to-preserve-the-activity-of-gonadotropins-in-liquid-form |
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