Phenotypic and Molecular Detection of Macrolide Lincosamide Streptogramin B Resistance in Clinical Isolates of Staphylococci
Introduction: Macrolide, lincosamide, and streptogramin B (MLSB) antibiotics are commonly used in the treatment of staphylococcal infections. Some genes like erm, msr, and lnu confer resistance to these group antibiotics. Resistance phenotype can be one of the constitutive, inducible or efflux pheno...
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| Format: | Article |
| Language: | English |
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Bilimsel Tip Yayinevi
2020-06-01
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| Series: | Flora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi |
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| Online Access: | http://www.floradergisi.org/managete/fu_folder/2020-02/2020-25-02-190-196.pdf |
| _version_ | 1797917538597732352 |
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| author | Güliz UYAR GÜLEÇ Serkan ÖNCÜ Bülent BOZDOĞAN Barçın ÖZTÜRK Bülent ERTUĞRUL Serhan SAKARYA |
| author_facet | Güliz UYAR GÜLEÇ Serkan ÖNCÜ Bülent BOZDOĞAN Barçın ÖZTÜRK Bülent ERTUĞRUL Serhan SAKARYA |
| author_sort | Güliz UYAR GÜLEÇ |
| collection | DOAJ |
| description | Introduction: Macrolide, lincosamide, and streptogramin B (MLSB) antibiotics are commonly used in the treatment of staphylococcal infections. Some genes like erm, msr, and lnu confer resistance to these group antibiotics. Resistance phenotype can be one of the constitutive, inducible or efflux phenotypes. Resistance rates have been reported at varying rates from various countries. This study aimed to detect MLSB resistance rates, phenotypes, and genotypes in clinical isolates of both Staphylococcus aureus and coagulase-negative staphylococci (CNS).
Materials and Methods: A total of 82 staphylococci strains comprising 65.8% (n= 54) S. aureus and 34.2% (n= 28) CNS were
identified by conventional methods and 16S rRNA polymerase chain reaction (PCR). Antibiotic susceptibility testing for erythromycin, lincomycin, gentamicin, fusidic acid, levofloxacin, vancomycin, and linezolid was performed by minimum inhibitory concentration (MIC) agar dilution method. Double-disc diffusion test (D-test) was applied to investigate MLSB resistance phenotypes. In erythromycin-resistant isolates, PCR was used to detect the presence of ermA, ermB, ermC, and msrA genes.
Results: Among 54 S. aureus strains, 51% (n= 28) were methicillin-resistant and 49% (n= 26) were methicillin-susceptible. Among 28 CNS, 50% (n= 14) were methicillin-resistant. Erythromycin, lincomycin, gentamicin, and levofloxacin resistance rates were higher in the methicillin-resistant group (p< 0.05). Erythromycin resistance rate was 54.9%. Rates of constitutive and inducible MLSB and MSB phenotypes were 30.5%, 18.3%, and 6.1%, respectively. The most prevalent resistance determinants were ermA (32.9%) and ermC (7.3%). In addition, gene combinations were detected.
Conclusion: Due to the wide geographic distribution of resistance phenotypes and genotypes, local statistics are of critical value for empiric therapy. Double-disc diffusion test is useful to guide interpretation of the susceptibility test. |
| first_indexed | 2024-04-10T13:14:42Z |
| format | Article |
| id | doaj.art-73bd7d1cedf64b95bf22145a469f0cbc |
| institution | Directory Open Access Journal |
| issn | 1300-932X 1300-932X |
| language | English |
| last_indexed | 2024-04-10T13:14:42Z |
| publishDate | 2020-06-01 |
| publisher | Bilimsel Tip Yayinevi |
| record_format | Article |
| series | Flora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi |
| spelling | doaj.art-73bd7d1cedf64b95bf22145a469f0cbc2023-02-15T16:12:25ZengBilimsel Tip YayineviFlora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi1300-932X1300-932X2020-06-0125219019610.5578/flora.68683Phenotypic and Molecular Detection of Macrolide Lincosamide Streptogramin B Resistance in Clinical Isolates of StaphylococciGüliz UYAR GÜLEÇ0https://orcid.org/0000-0002-8565-1042Serkan ÖNCÜ1https://orcid.org/0000-0002-7815-8620Bülent BOZDOĞAN2https://orcid.org/0000-0003-2469-9728Barçın ÖZTÜRK3https://orcid.org/0000-0003-3756-8276Bülent ERTUĞRUL4https://orcid.org/0000-0002-6372-6680Serhan SAKARYA5https://orcid.org/0000-0001-5252-9211Department of Infectious Diseases and Clinical Microbiology, Faculty of Medicine, University of Adnan Menderes, Aydın, TurkeyDepartment of Infectious Diseases and Clinical Microbiology, Faculty of Medicine, University of Adnan Menderes, Aydın, TurkeyDepartment of Medical Microbiology, Faculty of Medicine, University of Adnan Menderes, Aydın, TurkeyDepartment of Infectious Diseases and Clinical Microbiology, Faculty of Medicine, University of Adnan Menderes, Aydın, TurkeyDepartment of Infectious Diseases and Clinical Microbiology, Faculty of Medicine, University of Adnan Menderes, Aydın, TurkeyDepartment of Infectious Diseases and Clinical Microbiology, Faculty of Medicine, University of Adnan Menderes, Aydın, TurkeyIntroduction: Macrolide, lincosamide, and streptogramin B (MLSB) antibiotics are commonly used in the treatment of staphylococcal infections. Some genes like erm, msr, and lnu confer resistance to these group antibiotics. Resistance phenotype can be one of the constitutive, inducible or efflux phenotypes. Resistance rates have been reported at varying rates from various countries. This study aimed to detect MLSB resistance rates, phenotypes, and genotypes in clinical isolates of both Staphylococcus aureus and coagulase-negative staphylococci (CNS). Materials and Methods: A total of 82 staphylococci strains comprising 65.8% (n= 54) S. aureus and 34.2% (n= 28) CNS were identified by conventional methods and 16S rRNA polymerase chain reaction (PCR). Antibiotic susceptibility testing for erythromycin, lincomycin, gentamicin, fusidic acid, levofloxacin, vancomycin, and linezolid was performed by minimum inhibitory concentration (MIC) agar dilution method. Double-disc diffusion test (D-test) was applied to investigate MLSB resistance phenotypes. In erythromycin-resistant isolates, PCR was used to detect the presence of ermA, ermB, ermC, and msrA genes. Results: Among 54 S. aureus strains, 51% (n= 28) were methicillin-resistant and 49% (n= 26) were methicillin-susceptible. Among 28 CNS, 50% (n= 14) were methicillin-resistant. Erythromycin, lincomycin, gentamicin, and levofloxacin resistance rates were higher in the methicillin-resistant group (p< 0.05). Erythromycin resistance rate was 54.9%. Rates of constitutive and inducible MLSB and MSB phenotypes were 30.5%, 18.3%, and 6.1%, respectively. The most prevalent resistance determinants were ermA (32.9%) and ermC (7.3%). In addition, gene combinations were detected. Conclusion: Due to the wide geographic distribution of resistance phenotypes and genotypes, local statistics are of critical value for empiric therapy. Double-disc diffusion test is useful to guide interpretation of the susceptibility test.http://www.floradergisi.org/managete/fu_folder/2020-02/2020-25-02-190-196.pdfd-testerm genesmlsb resistancestaphylococci |
| spellingShingle | Güliz UYAR GÜLEÇ Serkan ÖNCÜ Bülent BOZDOĞAN Barçın ÖZTÜRK Bülent ERTUĞRUL Serhan SAKARYA Phenotypic and Molecular Detection of Macrolide Lincosamide Streptogramin B Resistance in Clinical Isolates of Staphylococci Flora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi d-test erm genes mlsb resistance staphylococci |
| title | Phenotypic and Molecular Detection of Macrolide Lincosamide Streptogramin B Resistance in Clinical Isolates of Staphylococci |
| title_full | Phenotypic and Molecular Detection of Macrolide Lincosamide Streptogramin B Resistance in Clinical Isolates of Staphylococci |
| title_fullStr | Phenotypic and Molecular Detection of Macrolide Lincosamide Streptogramin B Resistance in Clinical Isolates of Staphylococci |
| title_full_unstemmed | Phenotypic and Molecular Detection of Macrolide Lincosamide Streptogramin B Resistance in Clinical Isolates of Staphylococci |
| title_short | Phenotypic and Molecular Detection of Macrolide Lincosamide Streptogramin B Resistance in Clinical Isolates of Staphylococci |
| title_sort | phenotypic and molecular detection of macrolide lincosamide streptogramin b resistance in clinical isolates of staphylococci |
| topic | d-test erm genes mlsb resistance staphylococci |
| url | http://www.floradergisi.org/managete/fu_folder/2020-02/2020-25-02-190-196.pdf |
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