Host Specificity of the Dickeya Bacteriophage PP35 Is Directed by a Tail Spike Interaction With Bacterial O-Antigen, Enabling the Infection of Alternative Non-pathogenic Bacterial Host
Dickeya solani is a recently emerged virulent bacterial potato pathogen that poses a major threat to world agriculture. Because of increasing antibiotic resistance and growing limitations in antibiotic use, alternative antibacterials such as bacteriophages are being developed. Myoviridae bacteriopha...
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Frontiers Media S.A.
2019-01-01
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Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2018.03288/full |
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author | Anastasia P. Kabanova Anastasia P. Kabanova Mikhail M. Shneider Aleksei A. Korzhenkov Eugenia N. Bugaeva Kirill K. Miroshnikov Evelina L. Zdorovenko Eugene E. Kulikov Stepan V. Toschakov Stepan V. Toschakov Alexander N. Ignatov Yuriy A. Knirel Konstantin A. Miroshnikov Konstantin A. Miroshnikov |
author_facet | Anastasia P. Kabanova Anastasia P. Kabanova Mikhail M. Shneider Aleksei A. Korzhenkov Eugenia N. Bugaeva Kirill K. Miroshnikov Evelina L. Zdorovenko Eugene E. Kulikov Stepan V. Toschakov Stepan V. Toschakov Alexander N. Ignatov Yuriy A. Knirel Konstantin A. Miroshnikov Konstantin A. Miroshnikov |
author_sort | Anastasia P. Kabanova |
collection | DOAJ |
description | Dickeya solani is a recently emerged virulent bacterial potato pathogen that poses a major threat to world agriculture. Because of increasing antibiotic resistance and growing limitations in antibiotic use, alternative antibacterials such as bacteriophages are being developed. Myoviridae bacteriophages recently re-ranked as a separate Ackermannviridae family, such as phage PP35 described in this work, are the attractive candidates for this bacterial biocontrol. PP35 has a very specific host range due to the presence of tail spike protein PP35 gp156, which can depolymerize the O-polysaccharide (OPS) of D. solani. The D. solani OPS structure, →2)-β-D-6-deoxy-D-altrose-(1→, is so far unique among soft-rot Pectobacteriaceae, though it may exist in non-virulent environmental Enterobacteriaceae. The phage tail spike depolymerase degrades the shielding polysaccharide, and launches the cell infection process. We hypothesize that non-pathogenic commensal bacteria may maintain the population of the phage in soil environment. |
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institution | Directory Open Access Journal |
issn | 1664-302X |
language | English |
last_indexed | 2024-12-13T04:28:52Z |
publishDate | 2019-01-01 |
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series | Frontiers in Microbiology |
spelling | doaj.art-73bec96ba60446dcac6a6d8f7c7c956e2022-12-21T23:59:37ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2019-01-01910.3389/fmicb.2018.03288430806Host Specificity of the Dickeya Bacteriophage PP35 Is Directed by a Tail Spike Interaction With Bacterial O-Antigen, Enabling the Infection of Alternative Non-pathogenic Bacterial HostAnastasia P. Kabanova0Anastasia P. Kabanova1Mikhail M. Shneider2Aleksei A. Korzhenkov3Eugenia N. Bugaeva4Kirill K. Miroshnikov5Evelina L. Zdorovenko6Eugene E. Kulikov7Stepan V. Toschakov8Stepan V. Toschakov9Alexander N. Ignatov10Yuriy A. Knirel11Konstantin A. Miroshnikov12Konstantin A. Miroshnikov13Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, RussiaResearch Center “PhytoEngineering” Ltd., Rogachevo, RussiaShemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, RussiaImmanuel Kant Baltic Federal University, Kaliningrad, RussiaResearch Center “PhytoEngineering” Ltd., Rogachevo, RussiaWinogradsky Institute of Microbiology, Federal Research Center “Fundamentals of Biotechnology”, Russian Academy of Sciences, Moscow, RussiaZelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, RussiaWinogradsky Institute of Microbiology, Federal Research Center “Fundamentals of Biotechnology”, Russian Academy of Sciences, Moscow, RussiaImmanuel Kant Baltic Federal University, Kaliningrad, RussiaWinogradsky Institute of Microbiology, Federal Research Center “Fundamentals of Biotechnology”, Russian Academy of Sciences, Moscow, RussiaResearch Center “PhytoEngineering” Ltd., Rogachevo, RussiaZelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, RussiaShemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, RussiaResearch Center “PhytoEngineering” Ltd., Rogachevo, RussiaDickeya solani is a recently emerged virulent bacterial potato pathogen that poses a major threat to world agriculture. Because of increasing antibiotic resistance and growing limitations in antibiotic use, alternative antibacterials such as bacteriophages are being developed. Myoviridae bacteriophages recently re-ranked as a separate Ackermannviridae family, such as phage PP35 described in this work, are the attractive candidates for this bacterial biocontrol. PP35 has a very specific host range due to the presence of tail spike protein PP35 gp156, which can depolymerize the O-polysaccharide (OPS) of D. solani. The D. solani OPS structure, →2)-β-D-6-deoxy-D-altrose-(1→, is so far unique among soft-rot Pectobacteriaceae, though it may exist in non-virulent environmental Enterobacteriaceae. The phage tail spike depolymerase degrades the shielding polysaccharide, and launches the cell infection process. We hypothesize that non-pathogenic commensal bacteria may maintain the population of the phage in soil environment.https://www.frontiersin.org/article/10.3389/fmicb.2018.03288/fullbacteriophageDickeya solaniLelliottiagenomicstail spike proteinpolysaccharide |
spellingShingle | Anastasia P. Kabanova Anastasia P. Kabanova Mikhail M. Shneider Aleksei A. Korzhenkov Eugenia N. Bugaeva Kirill K. Miroshnikov Evelina L. Zdorovenko Eugene E. Kulikov Stepan V. Toschakov Stepan V. Toschakov Alexander N. Ignatov Yuriy A. Knirel Konstantin A. Miroshnikov Konstantin A. Miroshnikov Host Specificity of the Dickeya Bacteriophage PP35 Is Directed by a Tail Spike Interaction With Bacterial O-Antigen, Enabling the Infection of Alternative Non-pathogenic Bacterial Host Frontiers in Microbiology bacteriophage Dickeya solani Lelliottia genomics tail spike protein polysaccharide |
title | Host Specificity of the Dickeya Bacteriophage PP35 Is Directed by a Tail Spike Interaction With Bacterial O-Antigen, Enabling the Infection of Alternative Non-pathogenic Bacterial Host |
title_full | Host Specificity of the Dickeya Bacteriophage PP35 Is Directed by a Tail Spike Interaction With Bacterial O-Antigen, Enabling the Infection of Alternative Non-pathogenic Bacterial Host |
title_fullStr | Host Specificity of the Dickeya Bacteriophage PP35 Is Directed by a Tail Spike Interaction With Bacterial O-Antigen, Enabling the Infection of Alternative Non-pathogenic Bacterial Host |
title_full_unstemmed | Host Specificity of the Dickeya Bacteriophage PP35 Is Directed by a Tail Spike Interaction With Bacterial O-Antigen, Enabling the Infection of Alternative Non-pathogenic Bacterial Host |
title_short | Host Specificity of the Dickeya Bacteriophage PP35 Is Directed by a Tail Spike Interaction With Bacterial O-Antigen, Enabling the Infection of Alternative Non-pathogenic Bacterial Host |
title_sort | host specificity of the dickeya bacteriophage pp35 is directed by a tail spike interaction with bacterial o antigen enabling the infection of alternative non pathogenic bacterial host |
topic | bacteriophage Dickeya solani Lelliottia genomics tail spike protein polysaccharide |
url | https://www.frontiersin.org/article/10.3389/fmicb.2018.03288/full |
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