Host Specificity of the Dickeya Bacteriophage PP35 Is Directed by a Tail Spike Interaction With Bacterial O-Antigen, Enabling the Infection of Alternative Non-pathogenic Bacterial Host

Dickeya solani is a recently emerged virulent bacterial potato pathogen that poses a major threat to world agriculture. Because of increasing antibiotic resistance and growing limitations in antibiotic use, alternative antibacterials such as bacteriophages are being developed. Myoviridae bacteriopha...

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Main Authors: Anastasia P. Kabanova, Mikhail M. Shneider, Aleksei A. Korzhenkov, Eugenia N. Bugaeva, Kirill K. Miroshnikov, Evelina L. Zdorovenko, Eugene E. Kulikov, Stepan V. Toschakov, Alexander N. Ignatov, Yuriy A. Knirel, Konstantin A. Miroshnikov
Format: Article
Language:English
Published: Frontiers Media S.A. 2019-01-01
Series:Frontiers in Microbiology
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Online Access:https://www.frontiersin.org/article/10.3389/fmicb.2018.03288/full
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author Anastasia P. Kabanova
Anastasia P. Kabanova
Mikhail M. Shneider
Aleksei A. Korzhenkov
Eugenia N. Bugaeva
Kirill K. Miroshnikov
Evelina L. Zdorovenko
Eugene E. Kulikov
Stepan V. Toschakov
Stepan V. Toschakov
Alexander N. Ignatov
Yuriy A. Knirel
Konstantin A. Miroshnikov
Konstantin A. Miroshnikov
author_facet Anastasia P. Kabanova
Anastasia P. Kabanova
Mikhail M. Shneider
Aleksei A. Korzhenkov
Eugenia N. Bugaeva
Kirill K. Miroshnikov
Evelina L. Zdorovenko
Eugene E. Kulikov
Stepan V. Toschakov
Stepan V. Toschakov
Alexander N. Ignatov
Yuriy A. Knirel
Konstantin A. Miroshnikov
Konstantin A. Miroshnikov
author_sort Anastasia P. Kabanova
collection DOAJ
description Dickeya solani is a recently emerged virulent bacterial potato pathogen that poses a major threat to world agriculture. Because of increasing antibiotic resistance and growing limitations in antibiotic use, alternative antibacterials such as bacteriophages are being developed. Myoviridae bacteriophages recently re-ranked as a separate Ackermannviridae family, such as phage PP35 described in this work, are the attractive candidates for this bacterial biocontrol. PP35 has a very specific host range due to the presence of tail spike protein PP35 gp156, which can depolymerize the O-polysaccharide (OPS) of D. solani. The D. solani OPS structure, →2)-β-D-6-deoxy-D-altrose-(1→, is so far unique among soft-rot Pectobacteriaceae, though it may exist in non-virulent environmental Enterobacteriaceae. The phage tail spike depolymerase degrades the shielding polysaccharide, and launches the cell infection process. We hypothesize that non-pathogenic commensal bacteria may maintain the population of the phage in soil environment.
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spelling doaj.art-73bec96ba60446dcac6a6d8f7c7c956e2022-12-21T23:59:37ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2019-01-01910.3389/fmicb.2018.03288430806Host Specificity of the Dickeya Bacteriophage PP35 Is Directed by a Tail Spike Interaction With Bacterial O-Antigen, Enabling the Infection of Alternative Non-pathogenic Bacterial HostAnastasia P. Kabanova0Anastasia P. Kabanova1Mikhail M. Shneider2Aleksei A. Korzhenkov3Eugenia N. Bugaeva4Kirill K. Miroshnikov5Evelina L. Zdorovenko6Eugene E. Kulikov7Stepan V. Toschakov8Stepan V. Toschakov9Alexander N. Ignatov10Yuriy A. Knirel11Konstantin A. Miroshnikov12Konstantin A. Miroshnikov13Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, RussiaResearch Center “PhytoEngineering” Ltd., Rogachevo, RussiaShemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, RussiaImmanuel Kant Baltic Federal University, Kaliningrad, RussiaResearch Center “PhytoEngineering” Ltd., Rogachevo, RussiaWinogradsky Institute of Microbiology, Federal Research Center “Fundamentals of Biotechnology”, Russian Academy of Sciences, Moscow, RussiaZelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, RussiaWinogradsky Institute of Microbiology, Federal Research Center “Fundamentals of Biotechnology”, Russian Academy of Sciences, Moscow, RussiaImmanuel Kant Baltic Federal University, Kaliningrad, RussiaWinogradsky Institute of Microbiology, Federal Research Center “Fundamentals of Biotechnology”, Russian Academy of Sciences, Moscow, RussiaResearch Center “PhytoEngineering” Ltd., Rogachevo, RussiaZelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, RussiaShemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, RussiaResearch Center “PhytoEngineering” Ltd., Rogachevo, RussiaDickeya solani is a recently emerged virulent bacterial potato pathogen that poses a major threat to world agriculture. Because of increasing antibiotic resistance and growing limitations in antibiotic use, alternative antibacterials such as bacteriophages are being developed. Myoviridae bacteriophages recently re-ranked as a separate Ackermannviridae family, such as phage PP35 described in this work, are the attractive candidates for this bacterial biocontrol. PP35 has a very specific host range due to the presence of tail spike protein PP35 gp156, which can depolymerize the O-polysaccharide (OPS) of D. solani. The D. solani OPS structure, →2)-β-D-6-deoxy-D-altrose-(1→, is so far unique among soft-rot Pectobacteriaceae, though it may exist in non-virulent environmental Enterobacteriaceae. The phage tail spike depolymerase degrades the shielding polysaccharide, and launches the cell infection process. We hypothesize that non-pathogenic commensal bacteria may maintain the population of the phage in soil environment.https://www.frontiersin.org/article/10.3389/fmicb.2018.03288/fullbacteriophageDickeya solaniLelliottiagenomicstail spike proteinpolysaccharide
spellingShingle Anastasia P. Kabanova
Anastasia P. Kabanova
Mikhail M. Shneider
Aleksei A. Korzhenkov
Eugenia N. Bugaeva
Kirill K. Miroshnikov
Evelina L. Zdorovenko
Eugene E. Kulikov
Stepan V. Toschakov
Stepan V. Toschakov
Alexander N. Ignatov
Yuriy A. Knirel
Konstantin A. Miroshnikov
Konstantin A. Miroshnikov
Host Specificity of the Dickeya Bacteriophage PP35 Is Directed by a Tail Spike Interaction With Bacterial O-Antigen, Enabling the Infection of Alternative Non-pathogenic Bacterial Host
Frontiers in Microbiology
bacteriophage
Dickeya solani
Lelliottia
genomics
tail spike protein
polysaccharide
title Host Specificity of the Dickeya Bacteriophage PP35 Is Directed by a Tail Spike Interaction With Bacterial O-Antigen, Enabling the Infection of Alternative Non-pathogenic Bacterial Host
title_full Host Specificity of the Dickeya Bacteriophage PP35 Is Directed by a Tail Spike Interaction With Bacterial O-Antigen, Enabling the Infection of Alternative Non-pathogenic Bacterial Host
title_fullStr Host Specificity of the Dickeya Bacteriophage PP35 Is Directed by a Tail Spike Interaction With Bacterial O-Antigen, Enabling the Infection of Alternative Non-pathogenic Bacterial Host
title_full_unstemmed Host Specificity of the Dickeya Bacteriophage PP35 Is Directed by a Tail Spike Interaction With Bacterial O-Antigen, Enabling the Infection of Alternative Non-pathogenic Bacterial Host
title_short Host Specificity of the Dickeya Bacteriophage PP35 Is Directed by a Tail Spike Interaction With Bacterial O-Antigen, Enabling the Infection of Alternative Non-pathogenic Bacterial Host
title_sort host specificity of the dickeya bacteriophage pp35 is directed by a tail spike interaction with bacterial o antigen enabling the infection of alternative non pathogenic bacterial host
topic bacteriophage
Dickeya solani
Lelliottia
genomics
tail spike protein
polysaccharide
url https://www.frontiersin.org/article/10.3389/fmicb.2018.03288/full
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