Analysis of high density lipoprotein apolipoproteins by capillary zone and capillary SDS gel electrophoresis
We describe new methods for analyzing the apolipoproteins (apo) of the high density lipoproteins (HDL) of several species by two modes of capillary electrophoresis: size separation using a molecular sieving buffer, and capillary zone electrophoresis (CZE) using neutral coated capillaries. By either...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
1998-01-01
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| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520342176 |
| _version_ | 1818647432882290688 |
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| author | Joseph Stocks M. Nazeem Nanjee Norman E. Miller |
| author_facet | Joseph Stocks M. Nazeem Nanjee Norman E. Miller |
| author_sort | Joseph Stocks |
| collection | DOAJ |
| description | We describe new methods for analyzing the apolipoproteins (apo) of the high density lipoproteins (HDL) of several species by two modes of capillary electrophoresis: size separation using a molecular sieving buffer, and capillary zone electrophoresis (CZE) using neutral coated capillaries. By either mode HDL apos were resolved within 25 min. Results for apoA-I and apoA-II mass agreed with those by electroimmunoassay; intra-assay coefficients of variation were 1.8–4.2%. The migration times of human, rat, rabbit, and bovine apoA-I during CZE were proportional to their net charge/Mr ratios. This enabled human and rabbit apoA-I to be quantified simultaneously in transgenic rabbit HDLs. CZE also resolved human apoA-I isoforms, deamidated apoA-I, and pro-apoA-I.—Stocks, J., M. N. Nanjee, and N. E. Miller. Analysis of high density lipoprotein apolipoproteins by capillary zone and capillary SDS gel electrophoresis. J. Lipid Res. 1998. 39: 218–227. |
| first_indexed | 2024-12-17T01:02:27Z |
| format | Article |
| id | doaj.art-73c0f24759ad4467ae6c268f0c66d89c |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-17T01:02:27Z |
| publishDate | 1998-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-73c0f24759ad4467ae6c268f0c66d89c2022-12-21T22:09:24ZengElsevierJournal of Lipid Research0022-22751998-01-01391218227Analysis of high density lipoprotein apolipoproteins by capillary zone and capillary SDS gel electrophoresisJoseph Stocks0M. Nazeem Nanjee1Norman E. Miller2Department of Cardiovascular Biochemistry, St. Bartholomew's and the Royal London School of Medicine and Dentistry, Charterhouse Square, London, EC1M 6BQ, United KingdomDepartment of Cardiovascular Biochemistry, St. Bartholomew's and the Royal London School of Medicine and Dentistry, Charterhouse Square, London, EC1M 6BQ, United KingdomTo whom correspondence should be addressed.; Department of Cardiovascular Biochemistry, St. Bartholomew's and the Royal London School of Medicine and Dentistry, Charterhouse Square, London, EC1M 6BQ, United KingdomWe describe new methods for analyzing the apolipoproteins (apo) of the high density lipoproteins (HDL) of several species by two modes of capillary electrophoresis: size separation using a molecular sieving buffer, and capillary zone electrophoresis (CZE) using neutral coated capillaries. By either mode HDL apos were resolved within 25 min. Results for apoA-I and apoA-II mass agreed with those by electroimmunoassay; intra-assay coefficients of variation were 1.8–4.2%. The migration times of human, rat, rabbit, and bovine apoA-I during CZE were proportional to their net charge/Mr ratios. This enabled human and rabbit apoA-I to be quantified simultaneously in transgenic rabbit HDLs. CZE also resolved human apoA-I isoforms, deamidated apoA-I, and pro-apoA-I.—Stocks, J., M. N. Nanjee, and N. E. Miller. Analysis of high density lipoprotein apolipoproteins by capillary zone and capillary SDS gel electrophoresis. J. Lipid Res. 1998. 39: 218–227.http://www.sciencedirect.com/science/article/pii/S0022227520342176apolipoprotein A-Iapolipoprotein A-IIsodium dodecyl sulfatezone electrophoresisbovineimmunoelectrophoresis |
| spellingShingle | Joseph Stocks M. Nazeem Nanjee Norman E. Miller Analysis of high density lipoprotein apolipoproteins by capillary zone and capillary SDS gel electrophoresis Journal of Lipid Research apolipoprotein A-I apolipoprotein A-II sodium dodecyl sulfate zone electrophoresis bovine immunoelectrophoresis |
| title | Analysis of high density lipoprotein apolipoproteins by capillary zone and capillary SDS gel electrophoresis |
| title_full | Analysis of high density lipoprotein apolipoproteins by capillary zone and capillary SDS gel electrophoresis |
| title_fullStr | Analysis of high density lipoprotein apolipoproteins by capillary zone and capillary SDS gel electrophoresis |
| title_full_unstemmed | Analysis of high density lipoprotein apolipoproteins by capillary zone and capillary SDS gel electrophoresis |
| title_short | Analysis of high density lipoprotein apolipoproteins by capillary zone and capillary SDS gel electrophoresis |
| title_sort | analysis of high density lipoprotein apolipoproteins by capillary zone and capillary sds gel electrophoresis |
| topic | apolipoprotein A-I apolipoprotein A-II sodium dodecyl sulfate zone electrophoresis bovine immunoelectrophoresis |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520342176 |
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