Through Its Powerful Antioxidative Properties, L-Theanine Ameliorates Vincristine-Induced Neuropathy in Rats
L-theanine (LT), which is a major amino acid found in green tea, was shown to alleviate Vincristine (VCR)-induced peripheral neuropathy and associated neuronal functional changes in rats. To induce peripheral neuropathy, rats were administered VCR at a dose of 100 mg/kg/day intraperitoneally on days...
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MDPI AG
2023-03-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/12/4/803 |
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| author | Chih-Chuan Yang Mao-Hsien Wang Hung-Sheng Soung Hsiang-Chien Tseng Feng-Huei Lin Kuo-Chi Chang Cheng-Chia Tsai |
| author_facet | Chih-Chuan Yang Mao-Hsien Wang Hung-Sheng Soung Hsiang-Chien Tseng Feng-Huei Lin Kuo-Chi Chang Cheng-Chia Tsai |
| author_sort | Chih-Chuan Yang |
| collection | DOAJ |
| description | L-theanine (LT), which is a major amino acid found in green tea, was shown to alleviate Vincristine (VCR)-induced peripheral neuropathy and associated neuronal functional changes in rats. To induce peripheral neuropathy, rats were administered VCR at a dose of 100 mg/kg/day intraperitoneally on days 1–5 and 8–12, while control rats received LT at doses of 30, 100, and 300 mg/kg/day intraperitoneally for 21 days or saline solution. Electrophysiological measurements were taken to evaluate the nerve functional loss and recovery through motor and sensory nerve conduction velocities. The sciatic nerve was examined for several biomarkers, including nitric oxide (NO), malondialdehyde (MDA), glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), total calcium, IL-6, IL-10, MPO, and caspase-3. The results showed that VCR caused significant hyperalgesia and allodynia in rats; decreased nerve conduction velocity; increased NO and MDA levels; and decreased GSH, SOD, CAT, and IL-10 levels. LT was found to significantly reduce VCR-induced nociceptive pain thresholds, decrease oxidative stress levels (NO, MDA), increase antioxidative strength (GSH, SOD, CAT), and reduce neuroinflammatory activity and apoptosis markers (caspase-3). LT’s antioxidant, calcium homeostasis, anti-inflammatory, anti-apoptotic, and neuroprotective properties make it a potential adjuvant to conventional treatment in VCR-induced neuropathy in rats. |
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| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-11T05:18:00Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-73c1b4ae629043e6a61ee77332e7b7b12023-11-17T18:04:30ZengMDPI AGAntioxidants2076-39212023-03-0112480310.3390/antiox12040803Through Its Powerful Antioxidative Properties, L-Theanine Ameliorates Vincristine-Induced Neuropathy in RatsChih-Chuan Yang0Mao-Hsien Wang1Hung-Sheng Soung2Hsiang-Chien Tseng3Feng-Huei Lin4Kuo-Chi Chang5Cheng-Chia Tsai6Department of Neurosurgery, Mackay Memorial Hospital, Taipei 10449, TaiwanDepartment of Anesthesia, En Chu Kon Hospital, Sanshia District, New Taipei City 23702, TaiwanDepartment of Psychiatry, Yuan-Shan Br. of Taipei Veteran General Hospital, Yilan County 26604, TaiwanDepartment of Anesthesiology, Shin Kong Wu Ho-Su Memorial Hospital, Taipei 11101, TaiwanInstitute of Biomedical Engineering, National Taiwan University, Taipei 10051, TaiwanInstitute of Taiwan Instrument Research, National Applied Research Laboratories, Hsinchu 300092, TaiwanDepartment of Neurosurgery, Mackay Memorial Hospital, Taipei 10449, TaiwanL-theanine (LT), which is a major amino acid found in green tea, was shown to alleviate Vincristine (VCR)-induced peripheral neuropathy and associated neuronal functional changes in rats. To induce peripheral neuropathy, rats were administered VCR at a dose of 100 mg/kg/day intraperitoneally on days 1–5 and 8–12, while control rats received LT at doses of 30, 100, and 300 mg/kg/day intraperitoneally for 21 days or saline solution. Electrophysiological measurements were taken to evaluate the nerve functional loss and recovery through motor and sensory nerve conduction velocities. The sciatic nerve was examined for several biomarkers, including nitric oxide (NO), malondialdehyde (MDA), glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), total calcium, IL-6, IL-10, MPO, and caspase-3. The results showed that VCR caused significant hyperalgesia and allodynia in rats; decreased nerve conduction velocity; increased NO and MDA levels; and decreased GSH, SOD, CAT, and IL-10 levels. LT was found to significantly reduce VCR-induced nociceptive pain thresholds, decrease oxidative stress levels (NO, MDA), increase antioxidative strength (GSH, SOD, CAT), and reduce neuroinflammatory activity and apoptosis markers (caspase-3). LT’s antioxidant, calcium homeostasis, anti-inflammatory, anti-apoptotic, and neuroprotective properties make it a potential adjuvant to conventional treatment in VCR-induced neuropathy in rats.https://www.mdpi.com/2076-3921/12/4/803antioxidationVincristineL-theanineneuropathy |
| spellingShingle | Chih-Chuan Yang Mao-Hsien Wang Hung-Sheng Soung Hsiang-Chien Tseng Feng-Huei Lin Kuo-Chi Chang Cheng-Chia Tsai Through Its Powerful Antioxidative Properties, L-Theanine Ameliorates Vincristine-Induced Neuropathy in Rats Antioxidants antioxidation Vincristine L-theanine neuropathy |
| title | Through Its Powerful Antioxidative Properties, L-Theanine Ameliorates Vincristine-Induced Neuropathy in Rats |
| title_full | Through Its Powerful Antioxidative Properties, L-Theanine Ameliorates Vincristine-Induced Neuropathy in Rats |
| title_fullStr | Through Its Powerful Antioxidative Properties, L-Theanine Ameliorates Vincristine-Induced Neuropathy in Rats |
| title_full_unstemmed | Through Its Powerful Antioxidative Properties, L-Theanine Ameliorates Vincristine-Induced Neuropathy in Rats |
| title_short | Through Its Powerful Antioxidative Properties, L-Theanine Ameliorates Vincristine-Induced Neuropathy in Rats |
| title_sort | through its powerful antioxidative properties l theanine ameliorates vincristine induced neuropathy in rats |
| topic | antioxidation Vincristine L-theanine neuropathy |
| url | https://www.mdpi.com/2076-3921/12/4/803 |
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