Mutagenesis and Adaptation of the Psychrotrophic Fungus <i>Chrysosporium pannorum</i> A-1 as a Method for Improving β-pinene Bioconversion
Mutagenesis and adaptation of the psychrotrophic fungus <i>Chrysosporium pannorum</i> A-1 to the toxic substrate β-pinene were used to obtain a biocatalyst with increased resistance to this terpene and improved bioconversion properties. Mutants of the parental strain were induced with UV...
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MDPI AG
2020-06-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/25/11/2589 |
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| author | Mateusz Kutyła Jan Fiedurek Anna Gromada Krzysztof Jędrzejewski Mariusz Trytek |
| author_facet | Mateusz Kutyła Jan Fiedurek Anna Gromada Krzysztof Jędrzejewski Mariusz Trytek |
| author_sort | Mateusz Kutyła |
| collection | DOAJ |
| description | Mutagenesis and adaptation of the psychrotrophic fungus <i>Chrysosporium pannorum</i> A-1 to the toxic substrate β-pinene were used to obtain a biocatalyst with increased resistance to this terpene and improved bioconversion properties. Mutants of the parental strain were induced with UV light and <i>N</i>-methyl-<i>N</i>′-nitro-<i>N</i>-nitrosoguanidine. Mutants resistant to β-pinene were isolated using agar plates with a linear gradient of substrate concentrations. Active mutants were selected based on their general metabolic activity (GMA) expressed as oxygen consumption rate. Compared to the parental strain, the most active mutant showed an enhanced biotransformation ability to convert β-pinene to <i>trans</i>-pinocarveol (315 mg per g of dry mycelium), a 4.3-fold greater biocatalytic activity, and a higher resistance to H<sub>2</sub>O<sub>2</sub>-induced oxidative stress. Biotransformation using adapted mutants yielded twice as much <i>trans</i>-pinocarveol as the reaction catalyzed by non-adapted mutants. The results indicate that mutagenesis and adaptation of <i>C. pannorum</i> A-1 is an effective method of enhancing β-bioconversion of terpenes. |
| first_indexed | 2024-03-10T19:24:47Z |
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| id | doaj.art-73c43780329b41c4b1b4d829e85eec7b |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T19:24:47Z |
| publishDate | 2020-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-73c43780329b41c4b1b4d829e85eec7b2023-11-20T02:39:26ZengMDPI AGMolecules1420-30492020-06-012511258910.3390/molecules25112589Mutagenesis and Adaptation of the Psychrotrophic Fungus <i>Chrysosporium pannorum</i> A-1 as a Method for Improving β-pinene BioconversionMateusz Kutyła0Jan Fiedurek1Anna Gromada2Krzysztof Jędrzejewski3Mariusz Trytek4Department of Industrial and Environmental Microbiology, Faculty of Biology and Biotechnology, Maria Curie-Skłodowska University, Akademicka 19, 20-033 Lublin, PolandDepartment of Industrial and Environmental Microbiology, Faculty of Biology and Biotechnology, Maria Curie-Skłodowska University, Akademicka 19, 20-033 Lublin, PolandDepartment of Industrial and Environmental Microbiology, Faculty of Biology and Biotechnology, Maria Curie-Skłodowska University, Akademicka 19, 20-033 Lublin, PolandDepartment of Industrial and Environmental Microbiology, Faculty of Biology and Biotechnology, Maria Curie-Skłodowska University, Akademicka 19, 20-033 Lublin, PolandDepartment of Industrial and Environmental Microbiology, Faculty of Biology and Biotechnology, Maria Curie-Skłodowska University, Akademicka 19, 20-033 Lublin, PolandMutagenesis and adaptation of the psychrotrophic fungus <i>Chrysosporium pannorum</i> A-1 to the toxic substrate β-pinene were used to obtain a biocatalyst with increased resistance to this terpene and improved bioconversion properties. Mutants of the parental strain were induced with UV light and <i>N</i>-methyl-<i>N</i>′-nitro-<i>N</i>-nitrosoguanidine. Mutants resistant to β-pinene were isolated using agar plates with a linear gradient of substrate concentrations. Active mutants were selected based on their general metabolic activity (GMA) expressed as oxygen consumption rate. Compared to the parental strain, the most active mutant showed an enhanced biotransformation ability to convert β-pinene to <i>trans</i>-pinocarveol (315 mg per g of dry mycelium), a 4.3-fold greater biocatalytic activity, and a higher resistance to H<sub>2</sub>O<sub>2</sub>-induced oxidative stress. Biotransformation using adapted mutants yielded twice as much <i>trans</i>-pinocarveol as the reaction catalyzed by non-adapted mutants. The results indicate that mutagenesis and adaptation of <i>C. pannorum</i> A-1 is an effective method of enhancing β-bioconversion of terpenes.https://www.mdpi.com/1420-3049/25/11/2589adaptationbiotransformationUV/NTG mutagenesispsychrotrophsterpenes |
| spellingShingle | Mateusz Kutyła Jan Fiedurek Anna Gromada Krzysztof Jędrzejewski Mariusz Trytek Mutagenesis and Adaptation of the Psychrotrophic Fungus <i>Chrysosporium pannorum</i> A-1 as a Method for Improving β-pinene Bioconversion Molecules adaptation biotransformation UV/NTG mutagenesis psychrotrophs terpenes |
| title | Mutagenesis and Adaptation of the Psychrotrophic Fungus <i>Chrysosporium pannorum</i> A-1 as a Method for Improving β-pinene Bioconversion |
| title_full | Mutagenesis and Adaptation of the Psychrotrophic Fungus <i>Chrysosporium pannorum</i> A-1 as a Method for Improving β-pinene Bioconversion |
| title_fullStr | Mutagenesis and Adaptation of the Psychrotrophic Fungus <i>Chrysosporium pannorum</i> A-1 as a Method for Improving β-pinene Bioconversion |
| title_full_unstemmed | Mutagenesis and Adaptation of the Psychrotrophic Fungus <i>Chrysosporium pannorum</i> A-1 as a Method for Improving β-pinene Bioconversion |
| title_short | Mutagenesis and Adaptation of the Psychrotrophic Fungus <i>Chrysosporium pannorum</i> A-1 as a Method for Improving β-pinene Bioconversion |
| title_sort | mutagenesis and adaptation of the psychrotrophic fungus i chrysosporium pannorum i a 1 as a method for improving β pinene bioconversion |
| topic | adaptation biotransformation UV/NTG mutagenesis psychrotrophs terpenes |
| url | https://www.mdpi.com/1420-3049/25/11/2589 |
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