Deconstruction of Vermal Cerebellum in Ramp Locomotion in Mice
Abstract The cerebellum is involved in encoding balance, posture, speed, and gravity during locomotion. However, most studies are carried out on flat surfaces, and little is known about cerebellar activity during free ambulation on slopes. Here, it has been imaged the neuronal activity of cerebellar...
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| Format: | Article |
| Language: | English |
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Wiley
2023-01-01
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| Series: | Advanced Science |
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| Online Access: | https://doi.org/10.1002/advs.202203665 |
| _version_ | 1797962141832052736 |
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| author | Chenfei Lyu Chencen Yu Guanglong Sun Yue Zhao Ruolan Cai Hao Sun Xintai Wang Guoqiang Jia Lingzhu Fan Xi Chen Lin Zhou Ying Shen Lixia Gao Xinjian Li |
| author_facet | Chenfei Lyu Chencen Yu Guanglong Sun Yue Zhao Ruolan Cai Hao Sun Xintai Wang Guoqiang Jia Lingzhu Fan Xi Chen Lin Zhou Ying Shen Lixia Gao Xinjian Li |
| author_sort | Chenfei Lyu |
| collection | DOAJ |
| description | Abstract The cerebellum is involved in encoding balance, posture, speed, and gravity during locomotion. However, most studies are carried out on flat surfaces, and little is known about cerebellar activity during free ambulation on slopes. Here, it has been imaged the neuronal activity of cerebellar molecular interneurons (MLIs) and Purkinje cells (PCs) using a miniaturized microscope while a mouse is walking on a slope. It has been found that the neuronal activity of vermal MLIs specifically enhanced during uphill and downhill locomotion. In addition, a subset of MLIs is activated during entire uphill or downhill positions on the slope and is modulated by the slope inclines. In contrast, PCs showed counter‐balanced neuronal activity to MLIs, which reduced activity at the ramp peak. So, PCs may represent the ramp environment at the population level. In addition, chemogenetic inactivation of lobule V of the vermis impaired uphill locomotion. These results revealed a novel micro‐circuit in the vermal cerebellum that regulates ambulatory behavior in 3D terrains. |
| first_indexed | 2024-04-11T01:08:45Z |
| format | Article |
| id | doaj.art-73c5283e02cf4694bdd5a3037e5b735c |
| institution | Directory Open Access Journal |
| issn | 2198-3844 |
| language | English |
| last_indexed | 2024-04-11T01:08:45Z |
| publishDate | 2023-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advanced Science |
| spelling | doaj.art-73c5283e02cf4694bdd5a3037e5b735c2023-01-04T10:53:45ZengWileyAdvanced Science2198-38442023-01-01101n/an/a10.1002/advs.202203665Deconstruction of Vermal Cerebellum in Ramp Locomotion in MiceChenfei Lyu0Chencen Yu1Guanglong Sun2Yue Zhao3Ruolan Cai4Hao Sun5Xintai Wang6Guoqiang Jia7Lingzhu Fan8Xi Chen9Lin Zhou10Ying Shen11Lixia Gao12Xinjian Li13Department of Neurology of the Second Affiliated Hospital and Interdisciplinary Institute of Neuroscience and Technology Zhejiang University School of Medicine Hangzhou 310027 ChinaDepartment of Neurology of the Second Affiliated Hospital and Interdisciplinary Institute of Neuroscience and Technology Zhejiang University School of Medicine Hangzhou 310027 ChinaDepartment of Neurology of the Second Affiliated Hospital and Interdisciplinary Institute of Neuroscience and Technology Zhejiang University School of Medicine Hangzhou 310027 ChinaDepartment of Physiology and Department of Psychiatry Sir Run Run Shaw Hospital Zhejiang University School of Medicine Hangzhou 310058 ChinaDepartment of Neurology of the Second Affiliated Hospital and Interdisciplinary Institute of Neuroscience and Technology Zhejiang University School of Medicine Hangzhou 310027 ChinaDepartment of Neurology of the Second Affiliated Hospital and Interdisciplinary Institute of Neuroscience and Technology Zhejiang University School of Medicine Hangzhou 310027 ChinaDepartment of Physiology and Department of Psychiatry Sir Run Run Shaw Hospital Zhejiang University School of Medicine Hangzhou 310058 ChinaDepartment of Neurology of the Second Affiliated Hospital and Interdisciplinary Institute of Neuroscience and Technology Zhejiang University School of Medicine Hangzhou 310027 ChinaDepartment of Neurology of the Second Affiliated Hospital and Interdisciplinary Institute of Neuroscience and Technology Zhejiang University School of Medicine Hangzhou 310027 ChinaDepartment of Neuroscience City University of Hong Kong Kowloon Hong Kong ChinaDepartment of Physiology and Department of Psychiatry Sir Run Run Shaw Hospital Zhejiang University School of Medicine Hangzhou 310058 ChinaDepartment of Physiology and Department of Psychiatry Sir Run Run Shaw Hospital Zhejiang University School of Medicine Hangzhou 310058 ChinaDepartment of Neurology of the Second Affiliated Hospital and Interdisciplinary Institute of Neuroscience and Technology Zhejiang University School of Medicine Hangzhou 310027 ChinaDepartment of Neurology of the Second Affiliated Hospital and Interdisciplinary Institute of Neuroscience and Technology Zhejiang University School of Medicine Hangzhou 310027 ChinaAbstract The cerebellum is involved in encoding balance, posture, speed, and gravity during locomotion. However, most studies are carried out on flat surfaces, and little is known about cerebellar activity during free ambulation on slopes. Here, it has been imaged the neuronal activity of cerebellar molecular interneurons (MLIs) and Purkinje cells (PCs) using a miniaturized microscope while a mouse is walking on a slope. It has been found that the neuronal activity of vermal MLIs specifically enhanced during uphill and downhill locomotion. In addition, a subset of MLIs is activated during entire uphill or downhill positions on the slope and is modulated by the slope inclines. In contrast, PCs showed counter‐balanced neuronal activity to MLIs, which reduced activity at the ramp peak. So, PCs may represent the ramp environment at the population level. In addition, chemogenetic inactivation of lobule V of the vermis impaired uphill locomotion. These results revealed a novel micro‐circuit in the vermal cerebellum that regulates ambulatory behavior in 3D terrains.https://doi.org/10.1002/advs.202203665cerebellumdownhill locomotionmolecular interneuronsPurkinje cellsramp walkinguphill locomotion |
| spellingShingle | Chenfei Lyu Chencen Yu Guanglong Sun Yue Zhao Ruolan Cai Hao Sun Xintai Wang Guoqiang Jia Lingzhu Fan Xi Chen Lin Zhou Ying Shen Lixia Gao Xinjian Li Deconstruction of Vermal Cerebellum in Ramp Locomotion in Mice Advanced Science cerebellum downhill locomotion molecular interneurons Purkinje cells ramp walking uphill locomotion |
| title | Deconstruction of Vermal Cerebellum in Ramp Locomotion in Mice |
| title_full | Deconstruction of Vermal Cerebellum in Ramp Locomotion in Mice |
| title_fullStr | Deconstruction of Vermal Cerebellum in Ramp Locomotion in Mice |
| title_full_unstemmed | Deconstruction of Vermal Cerebellum in Ramp Locomotion in Mice |
| title_short | Deconstruction of Vermal Cerebellum in Ramp Locomotion in Mice |
| title_sort | deconstruction of vermal cerebellum in ramp locomotion in mice |
| topic | cerebellum downhill locomotion molecular interneurons Purkinje cells ramp walking uphill locomotion |
| url | https://doi.org/10.1002/advs.202203665 |
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