Characterization of Emerging Pathogens Carrying blaKPC-2 Gene in IncP-6 Plasmids Isolated From Urban Sewage in Argentina
Untreated wastewater is a reservoir for multidrug-resistant bacteria, but its role in the spread of antibiotic resistance in the human population remains poorly investigated. In this study, we isolated a KPC-2-producing ST2787 Klebsiella quasipneumoniae subsp. quasipneumoniae (WW14A), recovered from...
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Frontiers Media S.A.
2021-08-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2021.722536/full |
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| author | Barbara Ghiglione Barbara Ghiglione María Sol Haim Pedro Penzotti Florencia Brunetti Florencia Brunetti Gabriela D´Amico González Gabriela D´Amico González José Di Conza José Di Conza Roque Figueroa-Espinosa Roque Figueroa-Espinosa Lidia Nuñez María Tereza Pepe Razzolini Bruna Fuga Fernanda Esposito Maximiliano Vander Horden Nilton Lincopan Gabriel Gutkind Gabriel Gutkind Pablo Power Pablo Power Milena Dropa |
| author_facet | Barbara Ghiglione Barbara Ghiglione María Sol Haim Pedro Penzotti Florencia Brunetti Florencia Brunetti Gabriela D´Amico González Gabriela D´Amico González José Di Conza José Di Conza Roque Figueroa-Espinosa Roque Figueroa-Espinosa Lidia Nuñez María Tereza Pepe Razzolini Bruna Fuga Fernanda Esposito Maximiliano Vander Horden Nilton Lincopan Gabriel Gutkind Gabriel Gutkind Pablo Power Pablo Power Milena Dropa |
| author_sort | Barbara Ghiglione |
| collection | DOAJ |
| description | Untreated wastewater is a reservoir for multidrug-resistant bacteria, but its role in the spread of antibiotic resistance in the human population remains poorly investigated. In this study, we isolated a KPC-2-producing ST2787 Klebsiella quasipneumoniae subsp. quasipneumoniae (WW14A), recovered from raw sewage at a wastewater treatment plant in Argentina in 2018 and determined its complete genome sequence. Strain WW14A was resistant to all β-lactams, ciprofloxacin and amikacin. A core genome phylogenetic analysis indicated that WW14A was closely related to a GES-5-producing Taiwanese strain isolated from hospital wastewater in 2015 and it was clearly distinct from strains isolated recently in Argentina and Brazil. Interestingly, blaKPC-2 was harbored by a recently described IncP-6 broad-spectrum plasmid which was sporadically reported worldwide and had never been reported before in Argentina. We investigated the presence of the IncP-6 replicon in isolates obtained from the same sampling and found a novel non-typable/IncP-6 hybrid plasmid in a newly assigned ST1407 Enterobacter asburiae (WW19C) also harboring blaKPC-2. Nanopore sequencing and hybrid assembly of strains WW14A and WW19C revealed that both IncP-6 plasmids shared 72% of coverage (~20 kb), with 99.99% of sequence similarity and each one also presented uniquely combined regions that were derived from other plasmids recently reported in different countries of South America, Asia, and Europe. The region harboring the carbapenem resistance gene (~11 kb) in both plasmids contained a Tn3 transposon disrupted by a Tn3-ISApu-flanked element and the core sequence was composed by ΔISKpn6/blaKPC-2/ΔblaTEM-1/ISKpn27. Both strains also carried genes conferring resistance to heavy metals (e.g., arsenic, mercury, lead, cadmium, copper), pesticides (e.g., glyphosate), disinfectants, and several virulence-related genes, posing a potential pathogenic risk in the case of infections. This is the first study documenting blaKPC-2 associated with IncP-6 plasmids in K. quasipneumoniae and Enterobacter cloacae complex from wastewater in Argentina and highlights the circulation of IncP-6 plasmids as potential reservoirs of blaKPC-2 in the environment. |
| first_indexed | 2024-12-15T00:04:18Z |
| format | Article |
| id | doaj.art-73c658db3889476eacb04159de469d0e |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-12-15T00:04:18Z |
| publishDate | 2021-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-73c658db3889476eacb04159de469d0e2022-12-21T22:42:48ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882021-08-011110.3389/fcimb.2021.722536722536Characterization of Emerging Pathogens Carrying blaKPC-2 Gene in IncP-6 Plasmids Isolated From Urban Sewage in ArgentinaBarbara Ghiglione0Barbara Ghiglione1María Sol Haim2Pedro Penzotti3Florencia Brunetti4Florencia Brunetti5Gabriela D´Amico González6Gabriela D´Amico González7José Di Conza8José Di Conza9Roque Figueroa-Espinosa10Roque Figueroa-Espinosa11Lidia Nuñez12María Tereza Pepe Razzolini13Bruna Fuga14Fernanda Esposito15Maximiliano Vander Horden16Nilton Lincopan17Gabriel Gutkind18Gabriel Gutkind19Pablo Power20Pablo Power21Milena Dropa22Laboratorio de Resistencia Bacteriana, Instituto de Bacteriología y Virología Molecular (IBaViM), Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Ciudad Autónoma de Buenos Aires, ArgentinaConsejo Nacional de Investigaciones Científicas y Técnicas, Ciudad Autónoma de Buenos Aires, ArgentinaLaboratorio de Resistencia Bacteriana, Instituto de Bacteriología y Virología Molecular (IBaViM), Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Ciudad Autónoma de Buenos Aires, ArgentinaLaboratorio de Resistencia Bacteriana, Instituto de Bacteriología y Virología Molecular (IBaViM), Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Ciudad Autónoma de Buenos Aires, ArgentinaLaboratorio de Resistencia Bacteriana, Instituto de Bacteriología y Virología Molecular (IBaViM), Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Ciudad Autónoma de Buenos Aires, ArgentinaConsejo Nacional de Investigaciones Científicas y Técnicas, Ciudad Autónoma de Buenos Aires, ArgentinaLaboratorio de Resistencia Bacteriana, Instituto de Bacteriología y Virología Molecular (IBaViM), Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Ciudad Autónoma de Buenos Aires, ArgentinaConsejo Nacional de Investigaciones Científicas y Técnicas, Ciudad Autónoma de Buenos Aires, ArgentinaLaboratorio de Resistencia Bacteriana, Instituto de Bacteriología y Virología Molecular (IBaViM), Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Ciudad Autónoma de Buenos Aires, ArgentinaConsejo Nacional de Investigaciones Científicas y Técnicas, Ciudad Autónoma de Buenos Aires, ArgentinaLaboratorio de Resistencia Bacteriana, Instituto de Bacteriología y Virología Molecular (IBaViM), Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Ciudad Autónoma de Buenos Aires, ArgentinaConsejo Nacional de Investigaciones Científicas y Técnicas, Ciudad Autónoma de Buenos Aires, ArgentinaFacultad de Farmacia y Bioquímica, Cátedra de Salud Pública e Higiene Ambiental, Universidad de Buenos Aires, Ciudad Autónoma de Buenos Aires, ArgentinaDepartamento de Saúde Ambiental, Laboratório de Microbiologia Ambiental e Resistência Antimicrobiana - MicroRes, Faculdade de Saúde Pública, Universidade de São Paulo, São Paulo, BrazilDepartamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, BrazilDepartamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, BrazilIngeniería - Gerencia Técnica, Dirección de Saneamiento, Agua y Saneamientos Argentinos S.A. (AySA), Buenos Aires, ArgentinaDepartamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, BrazilLaboratorio de Resistencia Bacteriana, Instituto de Bacteriología y Virología Molecular (IBaViM), Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Ciudad Autónoma de Buenos Aires, ArgentinaConsejo Nacional de Investigaciones Científicas y Técnicas, Ciudad Autónoma de Buenos Aires, ArgentinaLaboratorio de Resistencia Bacteriana, Instituto de Bacteriología y Virología Molecular (IBaViM), Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Ciudad Autónoma de Buenos Aires, ArgentinaConsejo Nacional de Investigaciones Científicas y Técnicas, Ciudad Autónoma de Buenos Aires, ArgentinaDepartamento de Saúde Ambiental, Laboratório de Microbiologia Ambiental e Resistência Antimicrobiana - MicroRes, Faculdade de Saúde Pública, Universidade de São Paulo, São Paulo, BrazilUntreated wastewater is a reservoir for multidrug-resistant bacteria, but its role in the spread of antibiotic resistance in the human population remains poorly investigated. In this study, we isolated a KPC-2-producing ST2787 Klebsiella quasipneumoniae subsp. quasipneumoniae (WW14A), recovered from raw sewage at a wastewater treatment plant in Argentina in 2018 and determined its complete genome sequence. Strain WW14A was resistant to all β-lactams, ciprofloxacin and amikacin. A core genome phylogenetic analysis indicated that WW14A was closely related to a GES-5-producing Taiwanese strain isolated from hospital wastewater in 2015 and it was clearly distinct from strains isolated recently in Argentina and Brazil. Interestingly, blaKPC-2 was harbored by a recently described IncP-6 broad-spectrum plasmid which was sporadically reported worldwide and had never been reported before in Argentina. We investigated the presence of the IncP-6 replicon in isolates obtained from the same sampling and found a novel non-typable/IncP-6 hybrid plasmid in a newly assigned ST1407 Enterobacter asburiae (WW19C) also harboring blaKPC-2. Nanopore sequencing and hybrid assembly of strains WW14A and WW19C revealed that both IncP-6 plasmids shared 72% of coverage (~20 kb), with 99.99% of sequence similarity and each one also presented uniquely combined regions that were derived from other plasmids recently reported in different countries of South America, Asia, and Europe. The region harboring the carbapenem resistance gene (~11 kb) in both plasmids contained a Tn3 transposon disrupted by a Tn3-ISApu-flanked element and the core sequence was composed by ΔISKpn6/blaKPC-2/ΔblaTEM-1/ISKpn27. Both strains also carried genes conferring resistance to heavy metals (e.g., arsenic, mercury, lead, cadmium, copper), pesticides (e.g., glyphosate), disinfectants, and several virulence-related genes, posing a potential pathogenic risk in the case of infections. This is the first study documenting blaKPC-2 associated with IncP-6 plasmids in K. quasipneumoniae and Enterobacter cloacae complex from wastewater in Argentina and highlights the circulation of IncP-6 plasmids as potential reservoirs of blaKPC-2 in the environment.https://www.frontiersin.org/articles/10.3389/fcimb.2021.722536/fullKlebsiella quasipneumoniaeEnterobacter asburiaeKPC-2IncP-6wastewater |
| spellingShingle | Barbara Ghiglione Barbara Ghiglione María Sol Haim Pedro Penzotti Florencia Brunetti Florencia Brunetti Gabriela D´Amico González Gabriela D´Amico González José Di Conza José Di Conza Roque Figueroa-Espinosa Roque Figueroa-Espinosa Lidia Nuñez María Tereza Pepe Razzolini Bruna Fuga Fernanda Esposito Maximiliano Vander Horden Nilton Lincopan Gabriel Gutkind Gabriel Gutkind Pablo Power Pablo Power Milena Dropa Characterization of Emerging Pathogens Carrying blaKPC-2 Gene in IncP-6 Plasmids Isolated From Urban Sewage in Argentina Frontiers in Cellular and Infection Microbiology Klebsiella quasipneumoniae Enterobacter asburiae KPC-2 IncP-6 wastewater |
| title | Characterization of Emerging Pathogens Carrying blaKPC-2 Gene in IncP-6 Plasmids Isolated From Urban Sewage in Argentina |
| title_full | Characterization of Emerging Pathogens Carrying blaKPC-2 Gene in IncP-6 Plasmids Isolated From Urban Sewage in Argentina |
| title_fullStr | Characterization of Emerging Pathogens Carrying blaKPC-2 Gene in IncP-6 Plasmids Isolated From Urban Sewage in Argentina |
| title_full_unstemmed | Characterization of Emerging Pathogens Carrying blaKPC-2 Gene in IncP-6 Plasmids Isolated From Urban Sewage in Argentina |
| title_short | Characterization of Emerging Pathogens Carrying blaKPC-2 Gene in IncP-6 Plasmids Isolated From Urban Sewage in Argentina |
| title_sort | characterization of emerging pathogens carrying blakpc 2 gene in incp 6 plasmids isolated from urban sewage in argentina |
| topic | Klebsiella quasipneumoniae Enterobacter asburiae KPC-2 IncP-6 wastewater |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2021.722536/full |
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