Tracing clinically-relevant antimicrobial resistances in Acinetobacter baumannii-calcoaceticus complex across diverse environments: A study spanning clinical, livestock, and wastewater treatment settings
Acinetobacter baumannii has become a prominent nosocomial pathogen, primarily owing to its remarkable ability to rapidly acquire resistance to a wide range of antimicrobial agents and its ability to persist in diverse environments. However, there is a lack of data on the molecular epidemiology and i...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-04-01
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| Series: | Environment International |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0160412024001892 |
| _version_ | 1827275606492971008 |
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| author | Mykhailo Savin Esther Sib Celine Heinemann Vanessa M. Eichel Dennis Nurjadi Marian Klose Jens Andre Hammerl Ulrike Binsker Nico T. Mutters |
| author_facet | Mykhailo Savin Esther Sib Celine Heinemann Vanessa M. Eichel Dennis Nurjadi Marian Klose Jens Andre Hammerl Ulrike Binsker Nico T. Mutters |
| author_sort | Mykhailo Savin |
| collection | DOAJ |
| description | Acinetobacter baumannii has become a prominent nosocomial pathogen, primarily owing to its remarkable ability to rapidly acquire resistance to a wide range of antimicrobial agents and its ability to persist in diverse environments. However, there is a lack of data on the molecular epidemiology and its potential implications for public health of A. baumannii strains exhibiting clinically significant resistances that originate from non-clinical environments.Therefore, the genetic characteristics and resistance mechanisms of 80 A. baumannii-calcoaceticus (ABC) complex isolates, sourced from environments associated with poultry and pig production, municipal wastewater treatment plants (WWTPs), and clinical settings, were investigated.In total, our study classified 54 isolates into 29 previously described sequence types (STs), while 26 isolates exhibited as-yet-unassigned STs. We identified a broad range of A. baumannii STs originating from poultry and pig production environments (e.g., ST10, ST238, ST240, ST267, ST345, ST370, ST372, ST1112 according to Pasteur scheme). These STs have also been documented in clinical settings worldwide, highlighting their clinical significance. These findings also raise concerns about the potential zoonotic transmission of certain STs associated with livestock environments. Furthermore, we observed that clinical isolates exhibited the highest diversity of antimicrobial resistance genes (ARGs). In contrast to non-clinical isolates, clinical isolates typically carried a significantly higher number of ARGs, ranging from 10 to 15. They were also the exclusive carriers of biocide resistance genes and acquired carbapenemases (blaOXA-23, blaOXA-58, blaOXA-72, blaGIM-1, blaNDM-1). Additionally, we observed that clinical strains displayed an increased capacity for carrying plasmids and undergoing genetic transformation. This heightened capability could be linked to the intense selective pressures commonly found within clinical settings.Our study provides comprehensive insights into essential aspects of ABC isolates originating from livestock-associated environments and clinical settings. We explored their resistance mechanisms and potential implications for public health, providing valuable knowledge for addressing these critical issues. |
| first_indexed | 2024-04-24T16:50:41Z |
| format | Article |
| id | doaj.art-73c9c5c609ab4070a0efc85995c0e3d9 |
| institution | Directory Open Access Journal |
| issn | 0160-4120 |
| language | English |
| last_indexed | 2025-03-22T06:43:34Z |
| publishDate | 2024-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Environment International |
| spelling | doaj.art-73c9c5c609ab4070a0efc85995c0e3d92024-04-24T04:50:37ZengElsevierEnvironment International0160-41202024-04-01186108603Tracing clinically-relevant antimicrobial resistances in Acinetobacter baumannii-calcoaceticus complex across diverse environments: A study spanning clinical, livestock, and wastewater treatment settingsMykhailo Savin0Esther Sib1Celine Heinemann2Vanessa M. Eichel3Dennis Nurjadi4Marian Klose5Jens Andre Hammerl6Ulrike Binsker7Nico T. Mutters8Institute for Hygiene and Public Health, University Hospital Bonn, Bonn, Germany; Corresponding author at: Institute for Hygiene and Public Health, University Hospital Bonn, Venusberg-Campus 1 53127, Bonn, Germany.Institute for Hygiene and Public Health, University Hospital Bonn, Bonn, GermanyInstitute of Animal Sciences, University of Bonn, Bonn, GermanySection for Hospital Hygiene and Environmental Health, Center for Infectious Diseases, University Hospital Heidelberg, Heidelberg, GermanyDepartment of Infectious Diseases and Microbiology, University of Lübeck and University Medical Center Schleswig-Holstein Campus Lübeck, Lübeck, GermanyInstitute for Hygiene and Public Health, University Hospital Bonn, Bonn, GermanyDepartment for Biological Safety, German Federal Institute for Risk Assessment, Berlin, GermanyDepartment for Biological Safety, German Federal Institute for Risk Assessment, Berlin, GermanyInstitute for Hygiene and Public Health, University Hospital Bonn, Bonn, GermanyAcinetobacter baumannii has become a prominent nosocomial pathogen, primarily owing to its remarkable ability to rapidly acquire resistance to a wide range of antimicrobial agents and its ability to persist in diverse environments. However, there is a lack of data on the molecular epidemiology and its potential implications for public health of A. baumannii strains exhibiting clinically significant resistances that originate from non-clinical environments.Therefore, the genetic characteristics and resistance mechanisms of 80 A. baumannii-calcoaceticus (ABC) complex isolates, sourced from environments associated with poultry and pig production, municipal wastewater treatment plants (WWTPs), and clinical settings, were investigated.In total, our study classified 54 isolates into 29 previously described sequence types (STs), while 26 isolates exhibited as-yet-unassigned STs. We identified a broad range of A. baumannii STs originating from poultry and pig production environments (e.g., ST10, ST238, ST240, ST267, ST345, ST370, ST372, ST1112 according to Pasteur scheme). These STs have also been documented in clinical settings worldwide, highlighting their clinical significance. These findings also raise concerns about the potential zoonotic transmission of certain STs associated with livestock environments. Furthermore, we observed that clinical isolates exhibited the highest diversity of antimicrobial resistance genes (ARGs). In contrast to non-clinical isolates, clinical isolates typically carried a significantly higher number of ARGs, ranging from 10 to 15. They were also the exclusive carriers of biocide resistance genes and acquired carbapenemases (blaOXA-23, blaOXA-58, blaOXA-72, blaGIM-1, blaNDM-1). Additionally, we observed that clinical strains displayed an increased capacity for carrying plasmids and undergoing genetic transformation. This heightened capability could be linked to the intense selective pressures commonly found within clinical settings.Our study provides comprehensive insights into essential aspects of ABC isolates originating from livestock-associated environments and clinical settings. We explored their resistance mechanisms and potential implications for public health, providing valuable knowledge for addressing these critical issues.http://www.sciencedirect.com/science/article/pii/S0160412024001892One HealthA. baumanniiClinicsSlaughterhouseWastewaterTransformation |
| spellingShingle | Mykhailo Savin Esther Sib Celine Heinemann Vanessa M. Eichel Dennis Nurjadi Marian Klose Jens Andre Hammerl Ulrike Binsker Nico T. Mutters Tracing clinically-relevant antimicrobial resistances in Acinetobacter baumannii-calcoaceticus complex across diverse environments: A study spanning clinical, livestock, and wastewater treatment settings Environment International One Health A. baumannii Clinics Slaughterhouse Wastewater Transformation |
| title | Tracing clinically-relevant antimicrobial resistances in Acinetobacter baumannii-calcoaceticus complex across diverse environments: A study spanning clinical, livestock, and wastewater treatment settings |
| title_full | Tracing clinically-relevant antimicrobial resistances in Acinetobacter baumannii-calcoaceticus complex across diverse environments: A study spanning clinical, livestock, and wastewater treatment settings |
| title_fullStr | Tracing clinically-relevant antimicrobial resistances in Acinetobacter baumannii-calcoaceticus complex across diverse environments: A study spanning clinical, livestock, and wastewater treatment settings |
| title_full_unstemmed | Tracing clinically-relevant antimicrobial resistances in Acinetobacter baumannii-calcoaceticus complex across diverse environments: A study spanning clinical, livestock, and wastewater treatment settings |
| title_short | Tracing clinically-relevant antimicrobial resistances in Acinetobacter baumannii-calcoaceticus complex across diverse environments: A study spanning clinical, livestock, and wastewater treatment settings |
| title_sort | tracing clinically relevant antimicrobial resistances in acinetobacter baumannii calcoaceticus complex across diverse environments a study spanning clinical livestock and wastewater treatment settings |
| topic | One Health A. baumannii Clinics Slaughterhouse Wastewater Transformation |
| url | http://www.sciencedirect.com/science/article/pii/S0160412024001892 |
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