Expression of RASGRP2 in Lung Adenocarcinoma and Its Effect on Immune Microenvironment
Background and objective Lung cancer still has the highest incidence rate and mortality rate nowadays. In recent years, with the emergence of new drugs and the optimization of treatment mode, especially the clinical application of immunotherapy, the prognosis of lung cancer patients has been improve...
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| Format: | Article |
| Language: | zho |
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Chinese Anti-Cancer Association; Chinese Antituberculosis Association
2021-06-01
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| Series: | Chinese Journal of Lung Cancer |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.3779/j.issn.1009-3419.2021.105.01 |
| _version_ | 1818439329156956160 |
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| author | Shikang ZHAO Xin JIN Song XU |
| author_facet | Shikang ZHAO Xin JIN Song XU |
| author_sort | Shikang ZHAO |
| collection | DOAJ |
| description | Background and objective Lung cancer still has the highest incidence rate and mortality rate nowadays. In recent years, with the emergence of new drugs and the optimization of treatment mode, especially the clinical application of immunotherapy, the prognosis of lung cancer patients has been improved. However, the benefits of immunotherapy are still limited. Therefore, it is necessary to find new biomarkers to predict the prognosis of lung adenocarcinoma patients and explore its impact on the immune microenvironment. Methods The Cancer Genome Atlas (TCGA) database was used to analyze the gene sequencing and clinical data of patients with lung adenocarcinoma. The distribution of RASGRP2 in lung adenocarcinoma was determined by using the human protein mapping database. The Kaplan-Meier plotter database was used to explore the relationship between the expression of RASGRP2 and the prognosis of patients with lung adenocarcinoma. KEGG and GO gene enrichment analysis was performed in patients with high and low expression of RASGRP2. TCGA database was used to analyze the co-expression genes of RASGRP2 and TIMER database was used to calculate the immune related lymphoid infiltration of RASGRP2 and its coexpression genes. The relationship between RASGRP2 expression and immune checkpoint expression was analyzed by using TIMER 2.0 database. Results We found that RASGRP2 was low expressed in lung adenocarcinoma, and its expression level was related to the prognosis of patients. The high expression of RASGRP2 was involved in the process of hematopoietic cell formation and cell adhesion, and RASGRP2 played an important role in the process of T cell activation. Through TCGA database analysis, ZAP70, TBC1D10C, RASAL3, FGD2, CD37 and ACAP1 were significantly correlated with RASGRP2. The high expression of these genes leaded to the increase of the proportion of CD8+ T cells, memory CD4+ T cells, and the decrease of the proportion of neutrophils and Treg cells. Finally, we found that the expression of RASGRP2 was significantly correlated with the expression of CD274, CTLA4, LAG3 and TIGIT. Conclusion RASGRP2 was abnormally expressed in lung adenocarcinoma and correlated with the infiltration level of immune related cells, which might influence the efficacy of immunotherapy. |
| first_indexed | 2024-12-14T17:54:44Z |
| format | Article |
| id | doaj.art-73cdb4e976af4f678e4e70232bddf20f |
| institution | Directory Open Access Journal |
| issn | 1009-3419 1999-6187 |
| language | zho |
| last_indexed | 2024-12-14T17:54:44Z |
| publishDate | 2021-06-01 |
| publisher | Chinese Anti-Cancer Association; Chinese Antituberculosis Association |
| record_format | Article |
| series | Chinese Journal of Lung Cancer |
| spelling | doaj.art-73cdb4e976af4f678e4e70232bddf20f2022-12-21T22:52:34ZzhoChinese Anti-Cancer Association; Chinese Antituberculosis AssociationChinese Journal of Lung Cancer1009-34191999-61872021-06-0124640441110.3779/j.issn.1009-3419.2021.105.01Expression of RASGRP2 in Lung Adenocarcinoma and Its Effect
on Immune MicroenvironmentShikang ZHAO0Xin JIN1Song XU2Department of Lung Cancer Surgery; Tianjin Key Laboratory of Lung Cancer Metastasis and Tumor Microenvironment, Tianjin Lung Cancer Institute, Tianjin Medical University General Hospital, Tianjin 300052, ChinaDepartment of Lung Cancer Surgery; Tianjin Key Laboratory of Lung Cancer Metastasis and Tumor Microenvironment, Tianjin Lung Cancer Institute, Tianjin Medical University General Hospital, Tianjin 300052, ChinaDepartment of Lung Cancer Surgery; Tianjin Key Laboratory of Lung Cancer Metastasis and Tumor Microenvironment, Tianjin Lung Cancer Institute, Tianjin Medical University General Hospital, Tianjin 300052, ChinaBackground and objective Lung cancer still has the highest incidence rate and mortality rate nowadays. In recent years, with the emergence of new drugs and the optimization of treatment mode, especially the clinical application of immunotherapy, the prognosis of lung cancer patients has been improved. However, the benefits of immunotherapy are still limited. Therefore, it is necessary to find new biomarkers to predict the prognosis of lung adenocarcinoma patients and explore its impact on the immune microenvironment. Methods The Cancer Genome Atlas (TCGA) database was used to analyze the gene sequencing and clinical data of patients with lung adenocarcinoma. The distribution of RASGRP2 in lung adenocarcinoma was determined by using the human protein mapping database. The Kaplan-Meier plotter database was used to explore the relationship between the expression of RASGRP2 and the prognosis of patients with lung adenocarcinoma. KEGG and GO gene enrichment analysis was performed in patients with high and low expression of RASGRP2. TCGA database was used to analyze the co-expression genes of RASGRP2 and TIMER database was used to calculate the immune related lymphoid infiltration of RASGRP2 and its coexpression genes. The relationship between RASGRP2 expression and immune checkpoint expression was analyzed by using TIMER 2.0 database. Results We found that RASGRP2 was low expressed in lung adenocarcinoma, and its expression level was related to the prognosis of patients. The high expression of RASGRP2 was involved in the process of hematopoietic cell formation and cell adhesion, and RASGRP2 played an important role in the process of T cell activation. Through TCGA database analysis, ZAP70, TBC1D10C, RASAL3, FGD2, CD37 and ACAP1 were significantly correlated with RASGRP2. The high expression of these genes leaded to the increase of the proportion of CD8+ T cells, memory CD4+ T cells, and the decrease of the proportion of neutrophils and Treg cells. Finally, we found that the expression of RASGRP2 was significantly correlated with the expression of CD274, CTLA4, LAG3 and TIGIT. Conclusion RASGRP2 was abnormally expressed in lung adenocarcinoma and correlated with the infiltration level of immune related cells, which might influence the efficacy of immunotherapy.http://dx.doi.org/10.3779/j.issn.1009-3419.2021.105.01lung adenocarcinomaimmunotherapyrasgrp2prognosisimmune microenvironment |
| spellingShingle | Shikang ZHAO Xin JIN Song XU Expression of RASGRP2 in Lung Adenocarcinoma and Its Effect on Immune Microenvironment Chinese Journal of Lung Cancer lung adenocarcinoma immunotherapy rasgrp2 prognosis immune microenvironment |
| title | Expression of RASGRP2 in Lung Adenocarcinoma and Its Effect
on Immune Microenvironment |
| title_full | Expression of RASGRP2 in Lung Adenocarcinoma and Its Effect
on Immune Microenvironment |
| title_fullStr | Expression of RASGRP2 in Lung Adenocarcinoma and Its Effect
on Immune Microenvironment |
| title_full_unstemmed | Expression of RASGRP2 in Lung Adenocarcinoma and Its Effect
on Immune Microenvironment |
| title_short | Expression of RASGRP2 in Lung Adenocarcinoma and Its Effect
on Immune Microenvironment |
| title_sort | expression of rasgrp2 in lung adenocarcinoma and its effect
on immune microenvironment |
| topic | lung adenocarcinoma immunotherapy rasgrp2 prognosis immune microenvironment |
| url | http://dx.doi.org/10.3779/j.issn.1009-3419.2021.105.01 |
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