Molecular epidemiology and genetic diversity of Mycobacterium tuberculosis complex in referral health centers of Bamako, Mali: What is new?
Background and Aims: Tuberculosis (TB) remains an important global health issue worldwide. Despite this scourge threatening many human lives, especially in developing countries, thus far, no advanced molecular epidemiology study using recent and more accurate tools has been conducted in Mali. Theref...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-04-01
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| Series: | International Journal of Infectious Diseases |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1201971222000686 |
| _version_ | 1818755739205763072 |
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| author | Bourahima Kone Anou M. Somboro Mahamadou Kone Jane L. Holl Bocar Baya Djeneba Dabitao Dramane Diallo Bassirou Diarra Amadou Kone Yeya Dit Sadio Sarro Moumine Sanogo Antieme CG Togo Robert L. Murphy Souleymane Diallo Nadie Coulibaly Fatoumata Camara Seydou Samake Mahamadou Diakite Seydou Doumbia Mamoudou Maiga |
| author_facet | Bourahima Kone Anou M. Somboro Mahamadou Kone Jane L. Holl Bocar Baya Djeneba Dabitao Dramane Diallo Bassirou Diarra Amadou Kone Yeya Dit Sadio Sarro Moumine Sanogo Antieme CG Togo Robert L. Murphy Souleymane Diallo Nadie Coulibaly Fatoumata Camara Seydou Samake Mahamadou Diakite Seydou Doumbia Mamoudou Maiga |
| author_sort | Bourahima Kone |
| collection | DOAJ |
| description | Background and Aims: Tuberculosis (TB) remains an important global health issue worldwide. Despite this scourge threatening many human lives, especially in developing countries, thus far, no advanced molecular epidemiology study using recent and more accurate tools has been conducted in Mali. Therefore, this study aimed to use variable-number tandem repeats of mycobacterial interspersed repetitive units (MIRU-VNTR) technology coupled with the spoligotyping method to accurately determine the hot spots and establish the epidemiological transmission links of TB in Bamako, Mali. Methods: In a cross-sectional study, 245 isolates of Mycobacterium tuberculosis complex (MTBC) were characterized using spoligotyping and MIRU-VNTR, and an epidemiological investigation was conducted. Results: Of the 245 isolates, 184 (75.1%) were formally identified. The most widespread strain was the Cameroon strain (83; 45.1%). Eight major clusters were identified: Ghana (27; 14.7%), West African 2 (22; 12%), Haarlem (13; 7.1%), H37Rv (t) (8; 4.3%), Latin American Mediterranean (8; 4.3%), and Uganda I and II (6; 3.3%). Statistical analysis showed a significant difference between lineages from the respective referral health centers of Bamako, Mali (P = 0.01). Conclusion: This study establishes, for the first time, an accurate spatial distribution of circulating MTB strains in Bamako, Mali. The data was used to identify strains and “hot spots” causing TB infection and can also be used for more targeted public health responses, particularly for hot spots of drug-resistant strains. |
| first_indexed | 2024-12-18T05:43:56Z |
| format | Article |
| id | doaj.art-73d33dcc335242bea7e108a0de78d7ae |
| institution | Directory Open Access Journal |
| issn | 1201-9712 |
| language | English |
| last_indexed | 2024-12-18T05:43:56Z |
| publishDate | 2022-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | International Journal of Infectious Diseases |
| spelling | doaj.art-73d33dcc335242bea7e108a0de78d7ae2022-12-21T21:19:05ZengElsevierInternational Journal of Infectious Diseases1201-97122022-04-01117204211Molecular epidemiology and genetic diversity of Mycobacterium tuberculosis complex in referral health centers of Bamako, Mali: What is new?Bourahima Kone0Anou M. Somboro1Mahamadou Kone2Jane L. Holl3Bocar Baya4Djeneba Dabitao5Dramane Diallo6Bassirou Diarra7Amadou Kone8Yeya Dit Sadio Sarro9Moumine Sanogo10Antieme CG Togo11Robert L. Murphy12Souleymane Diallo13Nadie Coulibaly14Fatoumata Camara15Seydou Samake16Mahamadou Diakite17Seydou Doumbia18Mamoudou Maiga19University Clinical Research Center (UCRC) Laboratory, University of Sciences, Techniques and Technologies of Bamako (USTTB), Bamako, Mali; Corresponding author: Bourahima Kone, MSPH, University Clinical Research Center (UCRC), University of Sciences, Techniques and Technologies of Bamako (USTTB); BP. 1805; Tel: 00223 2022 6786/ Fax: 00223 2022 7513 and Mamoudou Maiga.University Clinical Research Center (UCRC) Laboratory, University of Sciences, Techniques and Technologies of Bamako (USTTB), Bamako, Mali; School of Laboratory Medicine and Medical Sciences, University of KwaZulu-Natal, Durban, South AfricaUniversity Clinical Research Center (UCRC) Laboratory, University of Sciences, Techniques and Technologies of Bamako (USTTB), Bamako, MaliCenter for Healthcare Delivery Science and Innovation, University of Chicago, Chicago, Illinois, USAUniversity Clinical Research Center (UCRC) Laboratory, University of Sciences, Techniques and Technologies of Bamako (USTTB), Bamako, MaliUniversity Clinical Research Center (UCRC) Laboratory, University of Sciences, Techniques and Technologies of Bamako (USTTB), Bamako, MaliUniversity Clinical Research Center (UCRC) Laboratory, University of Sciences, Techniques and Technologies of Bamako (USTTB), Bamako, MaliUniversity Clinical Research Center (UCRC) Laboratory, University of Sciences, Techniques and Technologies of Bamako (USTTB), Bamako, MaliUniversity Clinical Research Center (UCRC) Laboratory, University of Sciences, Techniques and Technologies of Bamako (USTTB), Bamako, MaliUniversity Clinical Research Center (UCRC) Laboratory, University of Sciences, Techniques and Technologies of Bamako (USTTB), Bamako, MaliUniversity Clinical Research Center (UCRC) Laboratory, University of Sciences, Techniques and Technologies of Bamako (USTTB), Bamako, MaliUniversity Clinical Research Center (UCRC) Laboratory, University of Sciences, Techniques and Technologies of Bamako (USTTB), Bamako, MaliBiomedical Engineering Department, Northwestern University, Evanston, Illinois, USAUniversity Clinical Research Center (UCRC) Laboratory, University of Sciences, Techniques and Technologies of Bamako (USTTB), Bamako, MaliUniversity Clinical Research Center (UCRC) Laboratory, University of Sciences, Techniques and Technologies of Bamako (USTTB), Bamako, MaliUniversity Clinical Research Center (UCRC) Laboratory, University of Sciences, Techniques and Technologies of Bamako (USTTB), Bamako, MaliUniversity Clinical Research Center (UCRC) Laboratory, University of Sciences, Techniques and Technologies of Bamako (USTTB), Bamako, MaliUniversity Clinical Research Center (UCRC) Laboratory, University of Sciences, Techniques and Technologies of Bamako (USTTB), Bamako, MaliUniversity Clinical Research Center (UCRC) Laboratory, University of Sciences, Techniques and Technologies of Bamako (USTTB), Bamako, MaliUniversity Clinical Research Center (UCRC) Laboratory, University of Sciences, Techniques and Technologies of Bamako (USTTB), Bamako, Mali; Biomedical Engineering Department, Northwestern University, Evanston, Illinois, USA; Corresponding author: Bourahima Kone, MSPH, University Clinical Research Center (UCRC), University of Sciences, Techniques and Technologies of Bamako (USTTB); BP. 1805; Tel: 00223 2022 6786/ Fax: 00223 2022 7513 and Mamoudou Maiga.Background and Aims: Tuberculosis (TB) remains an important global health issue worldwide. Despite this scourge threatening many human lives, especially in developing countries, thus far, no advanced molecular epidemiology study using recent and more accurate tools has been conducted in Mali. Therefore, this study aimed to use variable-number tandem repeats of mycobacterial interspersed repetitive units (MIRU-VNTR) technology coupled with the spoligotyping method to accurately determine the hot spots and establish the epidemiological transmission links of TB in Bamako, Mali. Methods: In a cross-sectional study, 245 isolates of Mycobacterium tuberculosis complex (MTBC) were characterized using spoligotyping and MIRU-VNTR, and an epidemiological investigation was conducted. Results: Of the 245 isolates, 184 (75.1%) were formally identified. The most widespread strain was the Cameroon strain (83; 45.1%). Eight major clusters were identified: Ghana (27; 14.7%), West African 2 (22; 12%), Haarlem (13; 7.1%), H37Rv (t) (8; 4.3%), Latin American Mediterranean (8; 4.3%), and Uganda I and II (6; 3.3%). Statistical analysis showed a significant difference between lineages from the respective referral health centers of Bamako, Mali (P = 0.01). Conclusion: This study establishes, for the first time, an accurate spatial distribution of circulating MTB strains in Bamako, Mali. The data was used to identify strains and “hot spots” causing TB infection and can also be used for more targeted public health responses, particularly for hot spots of drug-resistant strains.http://www.sciencedirect.com/science/article/pii/S1201971222000686TBGenetic diversitySpoligotypingMIRU-VNTRMali |
| spellingShingle | Bourahima Kone Anou M. Somboro Mahamadou Kone Jane L. Holl Bocar Baya Djeneba Dabitao Dramane Diallo Bassirou Diarra Amadou Kone Yeya Dit Sadio Sarro Moumine Sanogo Antieme CG Togo Robert L. Murphy Souleymane Diallo Nadie Coulibaly Fatoumata Camara Seydou Samake Mahamadou Diakite Seydou Doumbia Mamoudou Maiga Molecular epidemiology and genetic diversity of Mycobacterium tuberculosis complex in referral health centers of Bamako, Mali: What is new? International Journal of Infectious Diseases TB Genetic diversity Spoligotyping MIRU-VNTR Mali |
| title | Molecular epidemiology and genetic diversity of Mycobacterium tuberculosis complex in referral health centers of Bamako, Mali: What is new? |
| title_full | Molecular epidemiology and genetic diversity of Mycobacterium tuberculosis complex in referral health centers of Bamako, Mali: What is new? |
| title_fullStr | Molecular epidemiology and genetic diversity of Mycobacterium tuberculosis complex in referral health centers of Bamako, Mali: What is new? |
| title_full_unstemmed | Molecular epidemiology and genetic diversity of Mycobacterium tuberculosis complex in referral health centers of Bamako, Mali: What is new? |
| title_short | Molecular epidemiology and genetic diversity of Mycobacterium tuberculosis complex in referral health centers of Bamako, Mali: What is new? |
| title_sort | molecular epidemiology and genetic diversity of mycobacterium tuberculosis complex in referral health centers of bamako mali what is new |
| topic | TB Genetic diversity Spoligotyping MIRU-VNTR Mali |
| url | http://www.sciencedirect.com/science/article/pii/S1201971222000686 |
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