Fabrication of Carum copticum essential oil–loaded chitosan nanoparticles and evaluation its insecticidal activity for controlling Rhyzopertha dominica and Tribolium confusum
IntroductionPlant essential oils (EOs) can be used as a feasible tool for insect pest control. Nanoparticle formulations of plant EOs can improve the efficiency and stability of EOs, as well as insecticidal potential. MethodsIn this study, Carum copticum L. essential oil–loaded nanoparticles (OLNs)...
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| Format: | Article |
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Frontiers Media S.A.
2023-07-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2023.1187616/full |
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| author | Masumeh Ziaee Asiyeh Sheikhzadeh Takabi Asgar Ebadollahi |
| author_facet | Masumeh Ziaee Asiyeh Sheikhzadeh Takabi Asgar Ebadollahi |
| author_sort | Masumeh Ziaee |
| collection | DOAJ |
| description | IntroductionPlant essential oils (EOs) can be used as a feasible tool for insect pest control. Nanoparticle formulations of plant EOs can improve the efficiency and stability of EOs, as well as insecticidal potential. MethodsIn this study, Carum copticum L. essential oil–loaded nanoparticles (OLNs) were prepared via an oil-in-water emulsion, followed by droplet solidiffication via ionic gelation using a cross-linker, sodium tripolyphosphate (TPP). The nanoparticles were characterized by ultraviolet and visible (UV–Vis) spectrophotometry, Fourier-transform infrared spectroscopy (FTIR), laser light scattering (LS), transmission electron microscopy (TEM), and scanning electron microscopy (SEM). Moreover, the insecticidal activity of C. copticum EO and OLNs was evaluated against Rhyzopertha dominica (F.) (Coleoptera: Bostrychidae) and Tribolium confusum Jacquelin du Val. (Coleoptera: Tenebrionidae). In addition, their effectiveness was assessed on the progeny production of tested insect species.Results and discussionThe loading efficiency ranged from 34.33 to 84.16% when the chitosan to EO weight ratio was 1:1.25 and 1:0.5, respectively. The loading efficiency decreased with increasing EO content in the nanoparticles. The OLN particles exhibited spherical shape. The particle size was in the range 120–223.6 nm and increased with the increase of EO to chitosan ratio. So that the largest mean particle size (223.6 nm) was reported in the 1:1.25 weight ratio of chitosan to the EO. The mortality percentage of R. dominica and T. confusum adults were 74 and 57% when exposed for 7 days to 2000 mg/kg of OLNs at the 1:1.25 weight ratio, while EO caused 62 and 44% mortality on both insect species, respectively. Therefore, OLNs can potentially improve the insecticidal activity of C. copticum EO and could be applied to facilitate control of stored-product insect pests. |
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| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-03-12T21:22:46Z |
| publishDate | 2023-07-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Plant Science |
| spelling | doaj.art-73db3017f7f248c3879f4d22449c7ad42023-07-28T13:07:43ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-07-011410.3389/fpls.2023.11876161187616Fabrication of Carum copticum essential oil–loaded chitosan nanoparticles and evaluation its insecticidal activity for controlling Rhyzopertha dominica and Tribolium confusumMasumeh Ziaee0Asiyeh Sheikhzadeh Takabi1Asgar Ebadollahi2Department of Plant Protection, Faculty of Agriculture, Shahid Chamran University of Ahvaz, Ahvaz, IranDepartment of Chemistry, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, IranDepartment of Plant Sciences, Moghan College of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, IranIntroductionPlant essential oils (EOs) can be used as a feasible tool for insect pest control. Nanoparticle formulations of plant EOs can improve the efficiency and stability of EOs, as well as insecticidal potential. MethodsIn this study, Carum copticum L. essential oil–loaded nanoparticles (OLNs) were prepared via an oil-in-water emulsion, followed by droplet solidiffication via ionic gelation using a cross-linker, sodium tripolyphosphate (TPP). The nanoparticles were characterized by ultraviolet and visible (UV–Vis) spectrophotometry, Fourier-transform infrared spectroscopy (FTIR), laser light scattering (LS), transmission electron microscopy (TEM), and scanning electron microscopy (SEM). Moreover, the insecticidal activity of C. copticum EO and OLNs was evaluated against Rhyzopertha dominica (F.) (Coleoptera: Bostrychidae) and Tribolium confusum Jacquelin du Val. (Coleoptera: Tenebrionidae). In addition, their effectiveness was assessed on the progeny production of tested insect species.Results and discussionThe loading efficiency ranged from 34.33 to 84.16% when the chitosan to EO weight ratio was 1:1.25 and 1:0.5, respectively. The loading efficiency decreased with increasing EO content in the nanoparticles. The OLN particles exhibited spherical shape. The particle size was in the range 120–223.6 nm and increased with the increase of EO to chitosan ratio. So that the largest mean particle size (223.6 nm) was reported in the 1:1.25 weight ratio of chitosan to the EO. The mortality percentage of R. dominica and T. confusum adults were 74 and 57% when exposed for 7 days to 2000 mg/kg of OLNs at the 1:1.25 weight ratio, while EO caused 62 and 44% mortality on both insect species, respectively. Therefore, OLNs can potentially improve the insecticidal activity of C. copticum EO and could be applied to facilitate control of stored-product insect pests.https://www.frontiersin.org/articles/10.3389/fpls.2023.1187616/fullchitosanessential oilloading efficiencynanoparticlesstorage beetles |
| spellingShingle | Masumeh Ziaee Asiyeh Sheikhzadeh Takabi Asgar Ebadollahi Fabrication of Carum copticum essential oil–loaded chitosan nanoparticles and evaluation its insecticidal activity for controlling Rhyzopertha dominica and Tribolium confusum Frontiers in Plant Science chitosan essential oil loading efficiency nanoparticles storage beetles |
| title | Fabrication of Carum copticum essential oil–loaded chitosan nanoparticles and evaluation its insecticidal activity for controlling Rhyzopertha dominica and Tribolium confusum |
| title_full | Fabrication of Carum copticum essential oil–loaded chitosan nanoparticles and evaluation its insecticidal activity for controlling Rhyzopertha dominica and Tribolium confusum |
| title_fullStr | Fabrication of Carum copticum essential oil–loaded chitosan nanoparticles and evaluation its insecticidal activity for controlling Rhyzopertha dominica and Tribolium confusum |
| title_full_unstemmed | Fabrication of Carum copticum essential oil–loaded chitosan nanoparticles and evaluation its insecticidal activity for controlling Rhyzopertha dominica and Tribolium confusum |
| title_short | Fabrication of Carum copticum essential oil–loaded chitosan nanoparticles and evaluation its insecticidal activity for controlling Rhyzopertha dominica and Tribolium confusum |
| title_sort | fabrication of carum copticum essential oil loaded chitosan nanoparticles and evaluation its insecticidal activity for controlling rhyzopertha dominica and tribolium confusum |
| topic | chitosan essential oil loading efficiency nanoparticles storage beetles |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2023.1187616/full |
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