Lutzomyia umbratilis from an area south of the Negro River is refractory to in vitro interaction with Leishmania guyanensis
BACKGROUND Lutzomyia umbratilis, the vector for Leishmania guyanensis in northern South America, has been found naturally infected with L. guyanensis only in areas north of the Negro and Amazon rivers. While populations of this sand fly species are also found in areas south of these rivers, these po...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Fundação Oswaldo Cruz (FIOCRUZ)
|
| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762018000300202&lng=en&tlng=en |
| _version_ | 1797704663920803840 |
|---|---|
| author | Rodrigo Pedro Soares Paula Monalisa Nogueira Nágila Francinete Secundino Eric Fabrício Marialva Cláudia Maria Ríos-Velásquez Felipe Arley Costa Pessoa |
| author_facet | Rodrigo Pedro Soares Paula Monalisa Nogueira Nágila Francinete Secundino Eric Fabrício Marialva Cláudia Maria Ríos-Velásquez Felipe Arley Costa Pessoa |
| author_sort | Rodrigo Pedro Soares |
| collection | DOAJ |
| description | BACKGROUND Lutzomyia umbratilis, the vector for Leishmania guyanensis in northern South America, has been found naturally infected with L. guyanensis only in areas north of the Negro and Amazon rivers. While populations of this sand fly species are also found in areas south of these rivers, these populations have never been reported to be infected and/or transmitting L. guyanensis. However, no studies on the corresponding host-parasite interactions are available. OBJECTIVES This study evaluated the interaction between Lu. guyanensis promastigotes and field-collected Lu. umbratilis sand flies from Rio Preto da Eva and Manacapuru, which are located to the north and south, respectively, of the Negro River. METHODS Procyclic and metacyclic attachment was quantified using an in vitro system. FINDINGS Low attachment of parasites to the midguts of insects collected from Manacapuru was detected. Conversely, greater binding of metacyclic parasites was observed in the midguts of insects collected from Rio Preto da Eva, and this attachment was more pronounced than that observed for procyclics (p < 0.03). MAIN CONCLUSIONS The Lu. umbratilis population from an area south of the Negro River has lower in vitro interaction with L. guyanensis. The higher attachment of L. guyanensis to midguts of insects from Rio Preto da Eva may suggest better vector competence. These findings are in accordance with previously reported epidemiological information of American cutaneous leishmaniasis (ACL) transmission in the Amazon. |
| first_indexed | 2024-03-12T05:24:47Z |
| format | Article |
| id | doaj.art-73db3811c9794923abf035847860470e |
| institution | Directory Open Access Journal |
| issn | 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T05:24:47Z |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-73db3811c9794923abf035847860470e2023-09-03T07:25:05ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz1678-8060113320220510.1590/0074-02760170425S0074-02762018000300202Lutzomyia umbratilis from an area south of the Negro River is refractory to in vitro interaction with Leishmania guyanensisRodrigo Pedro SoaresPaula Monalisa NogueiraNágila Francinete SecundinoEric Fabrício MarialvaCláudia Maria Ríos-VelásquezFelipe Arley Costa PessoaBACKGROUND Lutzomyia umbratilis, the vector for Leishmania guyanensis in northern South America, has been found naturally infected with L. guyanensis only in areas north of the Negro and Amazon rivers. While populations of this sand fly species are also found in areas south of these rivers, these populations have never been reported to be infected and/or transmitting L. guyanensis. However, no studies on the corresponding host-parasite interactions are available. OBJECTIVES This study evaluated the interaction between Lu. guyanensis promastigotes and field-collected Lu. umbratilis sand flies from Rio Preto da Eva and Manacapuru, which are located to the north and south, respectively, of the Negro River. METHODS Procyclic and metacyclic attachment was quantified using an in vitro system. FINDINGS Low attachment of parasites to the midguts of insects collected from Manacapuru was detected. Conversely, greater binding of metacyclic parasites was observed in the midguts of insects collected from Rio Preto da Eva, and this attachment was more pronounced than that observed for procyclics (p < 0.03). MAIN CONCLUSIONS The Lu. umbratilis population from an area south of the Negro River has lower in vitro interaction with L. guyanensis. The higher attachment of L. guyanensis to midguts of insects from Rio Preto da Eva may suggest better vector competence. These findings are in accordance with previously reported epidemiological information of American cutaneous leishmaniasis (ACL) transmission in the Amazon.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762018000300202&lng=en&tlng=enLutzomyia umbratilisLeishmania guyanensishost-parasite interactionAmerican cutaneous leishmaniasis (ACL) |
| spellingShingle | Rodrigo Pedro Soares Paula Monalisa Nogueira Nágila Francinete Secundino Eric Fabrício Marialva Cláudia Maria Ríos-Velásquez Felipe Arley Costa Pessoa Lutzomyia umbratilis from an area south of the Negro River is refractory to in vitro interaction with Leishmania guyanensis Memorias do Instituto Oswaldo Cruz Lutzomyia umbratilis Leishmania guyanensis host-parasite interaction American cutaneous leishmaniasis (ACL) |
| title | Lutzomyia umbratilis from an area south of the Negro River is refractory to in vitro interaction with Leishmania guyanensis |
| title_full | Lutzomyia umbratilis from an area south of the Negro River is refractory to in vitro interaction with Leishmania guyanensis |
| title_fullStr | Lutzomyia umbratilis from an area south of the Negro River is refractory to in vitro interaction with Leishmania guyanensis |
| title_full_unstemmed | Lutzomyia umbratilis from an area south of the Negro River is refractory to in vitro interaction with Leishmania guyanensis |
| title_short | Lutzomyia umbratilis from an area south of the Negro River is refractory to in vitro interaction with Leishmania guyanensis |
| title_sort | lutzomyia umbratilis from an area south of the negro river is refractory to in vitro interaction with leishmania guyanensis |
| topic | Lutzomyia umbratilis Leishmania guyanensis host-parasite interaction American cutaneous leishmaniasis (ACL) |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762018000300202&lng=en&tlng=en |
| work_keys_str_mv | AT rodrigopedrosoares lutzomyiaumbratilisfromanareasouthofthenegroriverisrefractorytoinvitrointeractionwithleishmaniaguyanensis AT paulamonalisanogueira lutzomyiaumbratilisfromanareasouthofthenegroriverisrefractorytoinvitrointeractionwithleishmaniaguyanensis AT nagilafrancinetesecundino lutzomyiaumbratilisfromanareasouthofthenegroriverisrefractorytoinvitrointeractionwithleishmaniaguyanensis AT ericfabriciomarialva lutzomyiaumbratilisfromanareasouthofthenegroriverisrefractorytoinvitrointeractionwithleishmaniaguyanensis AT claudiamariariosvelasquez lutzomyiaumbratilisfromanareasouthofthenegroriverisrefractorytoinvitrointeractionwithleishmaniaguyanensis AT felipearleycostapessoa lutzomyiaumbratilisfromanareasouthofthenegroriverisrefractorytoinvitrointeractionwithleishmaniaguyanensis |