Evaluation of cytotoxic activity of Syringodium isoetifolium against human breast cancer cell line - an in silico and in vitro study
Seagrass is a natural, renewable, and much unexplored marine resource, which are capable, and reliable sources in the field of pharmaceuticals and drug discovery. The seagrass, Syringodium isoetifolium is our target plant for the study, which was collected from the Ramanathapuram district, Tamil Nad...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-10-01
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| Series: | Arabian Journal of Chemistry |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S187853522300641X |
| _version_ | 1797690930094931968 |
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| author | P. Kalaivani P. Amudha A. Chandramohan R. Vidya M. Prabhaharan P. Sasikumar Salim Albukhaty Ghassan M. Sulaiman Mosleh M. Abomughaid Mohammed H. Abu-Alghayth |
| author_facet | P. Kalaivani P. Amudha A. Chandramohan R. Vidya M. Prabhaharan P. Sasikumar Salim Albukhaty Ghassan M. Sulaiman Mosleh M. Abomughaid Mohammed H. Abu-Alghayth |
| author_sort | P. Kalaivani |
| collection | DOAJ |
| description | Seagrass is a natural, renewable, and much unexplored marine resource, which are capable, and reliable sources in the field of pharmaceuticals and drug discovery. The seagrass, Syringodium isoetifolium is our target plant for the study, which was collected from the Ramanathapuram district, Tamil Nadu. The current study is focused to evaluate the cytotoxic, cell cycle arrest, and apoptotic induction activities of the hydroalcoholic extract of S. isoetifolium against the human breast cancer (MCF-7) cell line. The cytotoxic evaluation revealed that the extract inhibited MCF-7 proliferation with IC50 value of 230.32 μg/ml. Evaluation of MCF-7 cell cycles demonstrated that the extract arrested the cell cycle in the S phase and continued to the G2/M phase at half of the IC50 value. The extract induced apoptotic of MCF-7 cells about 30.61% which was nearly the same with aripiprazole as a positive control (52.35%). Nootkatone significantly binds to the target proteins – HSP 90 and HER2 kinase with the least binding energy was predicted as the most active anticancer compounds by a molecular docking study. In light of the findings, it can be said that the hydroalcoholic extract of S. isoetifolium has the potential to be a therapeutic agent for breast malignancies by acting as an anticancer component. It can be used as an anticancer agent on its own and/or as a scaffold for analog synthesis to develop novel anticancer agents with improved therapeutic efficacy. |
| first_indexed | 2024-03-12T02:06:12Z |
| format | Article |
| id | doaj.art-73e0fbf61f1a4fb693d2e85fb4c38ef0 |
| institution | Directory Open Access Journal |
| issn | 1878-5352 |
| language | English |
| last_indexed | 2024-03-12T02:06:12Z |
| publishDate | 2023-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Arabian Journal of Chemistry |
| spelling | doaj.art-73e0fbf61f1a4fb693d2e85fb4c38ef02023-09-07T04:43:40ZengElsevierArabian Journal of Chemistry1878-53522023-10-011610105179Evaluation of cytotoxic activity of Syringodium isoetifolium against human breast cancer cell line - an in silico and in vitro studyP. Kalaivani0P. Amudha1A. Chandramohan2R. Vidya3M. Prabhaharan4P. Sasikumar5Salim Albukhaty6Ghassan M. Sulaiman7Mosleh M. Abomughaid8Mohammed H. Abu-Alghayth9Department of Biochemistry, Jaya College of Arts and Science, Thiruninravur 602024, Tamilnadu, IndiaDepartment of Biochemistry, Vels Institute of Science Technology and Advanced Studies, Pallavaram, Chennai 600117, Tamilnadu, India; Corresponding authors.Department of Biochemistry, Annai Violet Arts aXnd Science College, Affiliated to University of Madras, Chennai 600 053, Tamilnadu, IndiaDepartment of Biochemistry, Vels Institute of Science Technology and Advanced Studies, Pallavaram, Chennai 600117, Tamilnadu, IndiaDepartment of Physics, Saveetha School of Engineering, Saveetha Institute of Technical and Medical Sciences (SIMATS), Chennai 602105, Thandalam, Tamilnadu, India; Corresponding authors.Department of Physics, Saveetha School of Engineering, Saveetha Institute of Technical and Medical Sciences (SIMATS), Chennai 602105, Thandalam, Tamilnadu, India; Corresponding authors.Department of Chemistry, College of Science, University of Misan, Maysan 62001, Iraq; College of Medicine, University of Warith Al-Anbiyaa, Karbala 56001, IraqDivision of Biotechnology, Department of Applied Sciences, University of Technology, Baghdad 10066, Iraq; Corresponding authors.Department of Medical Laboratory Sciences, College of Applied Medical Sciences, University of Bisha, 255, Bisha 67714, Saudi ArabiaDepartment of Clinical Laboratory Sciences, Faculty of Applied Medical Sciences, University of Bisha, 255, Al Nakhil, Bisha 67714, Saudi ArabiaSeagrass is a natural, renewable, and much unexplored marine resource, which are capable, and reliable sources in the field of pharmaceuticals and drug discovery. The seagrass, Syringodium isoetifolium is our target plant for the study, which was collected from the Ramanathapuram district, Tamil Nadu. The current study is focused to evaluate the cytotoxic, cell cycle arrest, and apoptotic induction activities of the hydroalcoholic extract of S. isoetifolium against the human breast cancer (MCF-7) cell line. The cytotoxic evaluation revealed that the extract inhibited MCF-7 proliferation with IC50 value of 230.32 μg/ml. Evaluation of MCF-7 cell cycles demonstrated that the extract arrested the cell cycle in the S phase and continued to the G2/M phase at half of the IC50 value. The extract induced apoptotic of MCF-7 cells about 30.61% which was nearly the same with aripiprazole as a positive control (52.35%). Nootkatone significantly binds to the target proteins – HSP 90 and HER2 kinase with the least binding energy was predicted as the most active anticancer compounds by a molecular docking study. In light of the findings, it can be said that the hydroalcoholic extract of S. isoetifolium has the potential to be a therapeutic agent for breast malignancies by acting as an anticancer component. It can be used as an anticancer agent on its own and/or as a scaffold for analog synthesis to develop novel anticancer agents with improved therapeutic efficacy.http://www.sciencedirect.com/science/article/pii/S187853522300641XSyringodium isoetifoliumMCF-7ApoptosisInsilicoHSP 90 and HER2 Kinase receptorNootkatone and Zerumbone |
| spellingShingle | P. Kalaivani P. Amudha A. Chandramohan R. Vidya M. Prabhaharan P. Sasikumar Salim Albukhaty Ghassan M. Sulaiman Mosleh M. Abomughaid Mohammed H. Abu-Alghayth Evaluation of cytotoxic activity of Syringodium isoetifolium against human breast cancer cell line - an in silico and in vitro study Arabian Journal of Chemistry Syringodium isoetifolium MCF-7 Apoptosis Insilico HSP 90 and HER2 Kinase receptor Nootkatone and Zerumbone |
| title | Evaluation of cytotoxic activity of Syringodium isoetifolium against human breast cancer cell line - an in silico and in vitro study |
| title_full | Evaluation of cytotoxic activity of Syringodium isoetifolium against human breast cancer cell line - an in silico and in vitro study |
| title_fullStr | Evaluation of cytotoxic activity of Syringodium isoetifolium against human breast cancer cell line - an in silico and in vitro study |
| title_full_unstemmed | Evaluation of cytotoxic activity of Syringodium isoetifolium against human breast cancer cell line - an in silico and in vitro study |
| title_short | Evaluation of cytotoxic activity of Syringodium isoetifolium against human breast cancer cell line - an in silico and in vitro study |
| title_sort | evaluation of cytotoxic activity of syringodium isoetifolium against human breast cancer cell line an in silico and in vitro study |
| topic | Syringodium isoetifolium MCF-7 Apoptosis Insilico HSP 90 and HER2 Kinase receptor Nootkatone and Zerumbone |
| url | http://www.sciencedirect.com/science/article/pii/S187853522300641X |
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