Immunological Effects of Chitosan Nanoparticles on Breast Cancer Cells in Animal Model
Background and Objectives: Cancer is one of the leading causes of death around the world with a very high degree of mortality. Conventional therapies by cytotoxic drugs have high levels of side-effects for patient. Chitosan is a biocompatible and biodegradable compound. Thus, according to the minimu...
| Main Authors: | , |
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| Format: | Article |
| Language: | fas |
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Qom University of Medical Sciences
2017-04-01
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| Series: | Majallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Qum |
| Subjects: | |
| Online Access: | http://journal.muq.ac.ir/browse.php?a_code=A-10-154-1&slc_lang=en&sid=1 |
| _version_ | 1831669360292265984 |
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| author | Neda Soleimani Ashraf Mohabati Mobarez |
| author_facet | Neda Soleimani Ashraf Mohabati Mobarez |
| author_sort | Neda Soleimani |
| collection | DOAJ |
| description | Background and Objectives: Cancer is one of the leading causes of death around the world with a very high degree of mortality. Conventional therapies by cytotoxic drugs have high levels of side-effects for patient. Chitosan is a biocompatible and biodegradable compound. Thus, according to the minimum systemic toxicity for peptide and drug delivery, it is considered in applied programs. In this study, the Immunological effect of chitosan nanoparticles, was investigated on breast cancer cells in animal model.
Methods: In this experimental model, chitosan nanoparticles were synthesized. The size and electrical charge were determined using DLS apparatus and SEM. After creation of tumor model in female BALB/c mice. Injections in the test and control groups, were performed intraperitoneally. IL-4 and IFN-γ were assayed using ELISA method to investigate the immunomodulatory effects of the nanoparticles.
Results: Electric charge and size of the nanoparticles were determined and confirmed to be +11 mv and 200 nm, respectively. The nanoparticle-treated group produced large amounts of IFN-γ (213 pg/ml), while IL-4 level decreased (p<0.5).
Conclusion: These nanoparticles has the potential to shift immune responses from a Th2 to Th1 through IFN-γ production. Hence, these nanoparticles may be an appropriate and effective candidate for cancer therapy. |
| first_indexed | 2024-12-19T22:57:58Z |
| format | Article |
| id | doaj.art-73e3f3639f4f48f18efd8406e64aff2c |
| institution | Directory Open Access Journal |
| issn | 1735-7799 2008-1375 |
| language | fas |
| last_indexed | 2024-12-19T22:57:58Z |
| publishDate | 2017-04-01 |
| publisher | Qom University of Medical Sciences |
| record_format | Article |
| series | Majallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Qum |
| spelling | doaj.art-73e3f3639f4f48f18efd8406e64aff2c2022-12-21T20:02:36ZfasQom University of Medical SciencesMajallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Qum1735-77992008-13752017-04-0111219Immunological Effects of Chitosan Nanoparticles on Breast Cancer Cells in Animal ModelNeda Soleimani0Ashraf Mohabati Mobarez1 Shahid Beheshti University Tarbiat Modares University Background and Objectives: Cancer is one of the leading causes of death around the world with a very high degree of mortality. Conventional therapies by cytotoxic drugs have high levels of side-effects for patient. Chitosan is a biocompatible and biodegradable compound. Thus, according to the minimum systemic toxicity for peptide and drug delivery, it is considered in applied programs. In this study, the Immunological effect of chitosan nanoparticles, was investigated on breast cancer cells in animal model. Methods: In this experimental model, chitosan nanoparticles were synthesized. The size and electrical charge were determined using DLS apparatus and SEM. After creation of tumor model in female BALB/c mice. Injections in the test and control groups, were performed intraperitoneally. IL-4 and IFN-γ were assayed using ELISA method to investigate the immunomodulatory effects of the nanoparticles. Results: Electric charge and size of the nanoparticles were determined and confirmed to be +11 mv and 200 nm, respectively. The nanoparticle-treated group produced large amounts of IFN-γ (213 pg/ml), while IL-4 level decreased (p<0.5). Conclusion: These nanoparticles has the potential to shift immune responses from a Th2 to Th1 through IFN-γ production. Hence, these nanoparticles may be an appropriate and effective candidate for cancer therapy.http://journal.muq.ac.ir/browse.php?a_code=A-10-154-1&slc_lang=en&sid=1neoplasms nanoparticles immune system |
| spellingShingle | Neda Soleimani Ashraf Mohabati Mobarez Immunological Effects of Chitosan Nanoparticles on Breast Cancer Cells in Animal Model Majallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Qum neoplasms nanoparticles immune system |
| title | Immunological Effects of Chitosan Nanoparticles on Breast Cancer Cells in Animal Model |
| title_full | Immunological Effects of Chitosan Nanoparticles on Breast Cancer Cells in Animal Model |
| title_fullStr | Immunological Effects of Chitosan Nanoparticles on Breast Cancer Cells in Animal Model |
| title_full_unstemmed | Immunological Effects of Chitosan Nanoparticles on Breast Cancer Cells in Animal Model |
| title_short | Immunological Effects of Chitosan Nanoparticles on Breast Cancer Cells in Animal Model |
| title_sort | immunological effects of chitosan nanoparticles on breast cancer cells in animal model |
| topic | neoplasms nanoparticles immune system |
| url | http://journal.muq.ac.ir/browse.php?a_code=A-10-154-1&slc_lang=en&sid=1 |
| work_keys_str_mv | AT nedasoleimani immunologicaleffectsofchitosannanoparticlesonbreastcancercellsinanimalmodel AT ashrafmohabatimobarez immunologicaleffectsofchitosannanoparticlesonbreastcancercellsinanimalmodel |