Incorporation of bridged nucleic acids into CRISPR RNAs improves Cas9 endonuclease specificity

Incorporation of bridged nucleic acids into CRISPR RNAs improves Cas9 endonuclease specificity

Minimizing off-target effects is an important concern for therapeutic applications of CRISPR-Cas9. Here, the authors show that incorporating bridged or locked nucleic acids into crRNA improves editing kinetics and reduces off-target cleavage.

Bibliographic Details
Main Authors: Christopher R. Cromwell, Keewon Sung, Jinho Park, Amanda R. Krysler, Juan Jovel, Seong Keun Kim, Basil P. Hubbard
Format: Article
Language:English
Published: Nature Portfolio 2018-04-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-018-03927-0

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