TSPY potentiates cell proliferation and tumorigenesis by promoting cell cycle progression in HeLa and NIH3T3 cells
<p>Abstract</p> <p>Background</p> <p>TSPY is a repeated gene mapped to the critical region harboring the gonadoblastoma locus on the Y chromosome (GBY), the only oncogenic locus on this male-specific chromosome. Elevated levels of TSPY have been observed in gonadoblasto...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2006-06-01
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| Series: | BMC Cancer |
| Online Access: | http://www.biomedcentral.com/1471-2407/6/154 |
| _version_ | 1818530096247472128 |
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| author | Chan Wai-Yee Lee Tin-Lap Liu Xing Oram Shane W Lau Yun-Fai |
| author_facet | Chan Wai-Yee Lee Tin-Lap Liu Xing Oram Shane W Lau Yun-Fai |
| author_sort | Chan Wai-Yee |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>TSPY is a repeated gene mapped to the critical region harboring the gonadoblastoma locus on the Y chromosome (GBY), the only oncogenic locus on this male-specific chromosome. Elevated levels of TSPY have been observed in gonadoblastoma specimens and a variety of other tumor tissues, including testicular germ cell tumors, prostate cancer, melanoma, and liver cancer. TSPY contains a SET/NAP domain that is present in a family of cyclin B and/or histone binding proteins represented by the oncoprotein SET and the nucleosome assembly protein 1 (NAP1), involved in cell cycle regulation and replication.</p> <p>Methods</p> <p>To determine a possible cellular function for TSPY, we manipulated the TSPY expression in HeLa and NIH3T3 cells using the Tet-off system. Cell proliferation, colony formation assays and tumor growth in nude mice were utilized to determine the TSPY effects on cell growth and tumorigenesis. Cell cycle analysis and cell synchronization techniques were used to determine cell cycle profiles. Microarray and RT-PCR were used to investigate gene expression in TSPY expressing cells.</p> <p>Results</p> <p>Our findings suggest that TSPY expression increases cell proliferation <it>in vitro </it>and tumorigenesis <it>in vivo</it>. Ectopic expression of TSPY results in a smaller population of the host cells in the G<sub>2</sub>/M phase of the cell cycle. Using cell synchronization techniques, we show that TSPY is capable of mediating a rapid transition of the cells through the G<sub>2</sub>/M phase. Microarray analysis demonstrates that numerous genes involved in the cell cycle and apoptosis are affected by TSPY expression in the HeLa cells.</p> <p>Conclusion</p> <p>These data, taken together, have provided important insights on the probable functions of TSPY in cell cycle progression, cell proliferation, and tumorigenesis.</p> |
| first_indexed | 2024-12-11T17:15:25Z |
| format | Article |
| id | doaj.art-73ec03dc45e948688f1e9f2d0921021c |
| institution | Directory Open Access Journal |
| issn | 1471-2407 |
| language | English |
| last_indexed | 2024-12-11T17:15:25Z |
| publishDate | 2006-06-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cancer |
| spelling | doaj.art-73ec03dc45e948688f1e9f2d0921021c2022-12-22T00:57:21ZengBMCBMC Cancer1471-24072006-06-016115410.1186/1471-2407-6-154TSPY potentiates cell proliferation and tumorigenesis by promoting cell cycle progression in HeLa and NIH3T3 cellsChan Wai-YeeLee Tin-LapLiu XingOram Shane WLau Yun-Fai<p>Abstract</p> <p>Background</p> <p>TSPY is a repeated gene mapped to the critical region harboring the gonadoblastoma locus on the Y chromosome (GBY), the only oncogenic locus on this male-specific chromosome. Elevated levels of TSPY have been observed in gonadoblastoma specimens and a variety of other tumor tissues, including testicular germ cell tumors, prostate cancer, melanoma, and liver cancer. TSPY contains a SET/NAP domain that is present in a family of cyclin B and/or histone binding proteins represented by the oncoprotein SET and the nucleosome assembly protein 1 (NAP1), involved in cell cycle regulation and replication.</p> <p>Methods</p> <p>To determine a possible cellular function for TSPY, we manipulated the TSPY expression in HeLa and NIH3T3 cells using the Tet-off system. Cell proliferation, colony formation assays and tumor growth in nude mice were utilized to determine the TSPY effects on cell growth and tumorigenesis. Cell cycle analysis and cell synchronization techniques were used to determine cell cycle profiles. Microarray and RT-PCR were used to investigate gene expression in TSPY expressing cells.</p> <p>Results</p> <p>Our findings suggest that TSPY expression increases cell proliferation <it>in vitro </it>and tumorigenesis <it>in vivo</it>. Ectopic expression of TSPY results in a smaller population of the host cells in the G<sub>2</sub>/M phase of the cell cycle. Using cell synchronization techniques, we show that TSPY is capable of mediating a rapid transition of the cells through the G<sub>2</sub>/M phase. Microarray analysis demonstrates that numerous genes involved in the cell cycle and apoptosis are affected by TSPY expression in the HeLa cells.</p> <p>Conclusion</p> <p>These data, taken together, have provided important insights on the probable functions of TSPY in cell cycle progression, cell proliferation, and tumorigenesis.</p>http://www.biomedcentral.com/1471-2407/6/154 |
| spellingShingle | Chan Wai-Yee Lee Tin-Lap Liu Xing Oram Shane W Lau Yun-Fai TSPY potentiates cell proliferation and tumorigenesis by promoting cell cycle progression in HeLa and NIH3T3 cells BMC Cancer |
| title | TSPY potentiates cell proliferation and tumorigenesis by promoting cell cycle progression in HeLa and NIH3T3 cells |
| title_full | TSPY potentiates cell proliferation and tumorigenesis by promoting cell cycle progression in HeLa and NIH3T3 cells |
| title_fullStr | TSPY potentiates cell proliferation and tumorigenesis by promoting cell cycle progression in HeLa and NIH3T3 cells |
| title_full_unstemmed | TSPY potentiates cell proliferation and tumorigenesis by promoting cell cycle progression in HeLa and NIH3T3 cells |
| title_short | TSPY potentiates cell proliferation and tumorigenesis by promoting cell cycle progression in HeLa and NIH3T3 cells |
| title_sort | tspy potentiates cell proliferation and tumorigenesis by promoting cell cycle progression in hela and nih3t3 cells |
| url | http://www.biomedcentral.com/1471-2407/6/154 |
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