A Ferrofluid with Surface Modified Nanoparticles for Magnetic Hyperthermia and High ROS Production
A ferrofluid with 1,2-Benzenediol-coated iron oxide nanoparticles was synthesized and physicochemically analyzed. This colloidal system was prepared following the typical co-precipitation method, and superparamagnetic nanoparticles of 13.5 nm average diameter, 34 emu/g of magnetic saturation, and 28...
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| Language: | English |
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MDPI AG
2022-01-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/2/544 |
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| author | Oscar Cervantes Zaira del Rocio Lopez Norberto Casillas Peter Knauth Nayeli Checa Francisco Apolinar Cholico Rodolfo Hernandez-Gutiérrez Luis Hector Quintero Jose Avila Paz Mario Eduardo Cano |
| author_facet | Oscar Cervantes Zaira del Rocio Lopez Norberto Casillas Peter Knauth Nayeli Checa Francisco Apolinar Cholico Rodolfo Hernandez-Gutiérrez Luis Hector Quintero Jose Avila Paz Mario Eduardo Cano |
| author_sort | Oscar Cervantes |
| collection | DOAJ |
| description | A ferrofluid with 1,2-Benzenediol-coated iron oxide nanoparticles was synthesized and physicochemically analyzed. This colloidal system was prepared following the typical co-precipitation method, and superparamagnetic nanoparticles of 13.5 nm average diameter, 34 emu/g of magnetic saturation, and 285 K of blocking temperature were obtained. Additionally, the zeta potential showed a suitable colloidal stability for cancer therapy assays and the magneto-calorimetric trails determined a high power absorption density. In addition, the oxidative capability of the ferrofluid was corroborated by performing the Fenton reaction with methylene blue (MB) dissolved in water, where the ferrofluid was suitable for producing reactive oxygen species (ROS), and surprisingly a strong degradation of MB was also observed when it was combined with H<sub>2</sub>O<sub>2</sub>. The intracellular ROS production was qualitatively corroborated using the HT-29 human cell line, by detecting the fluorescent rise induced in 2,7-dichlorofluorescein diacetate. In other experiments, cell metabolic activity was measured, and no toxicity was observed, even with concentrations of up to 4 mg/mL of magnetic nanoparticles (MNPs). When the cells were treated with magnetic hyperthermia, 80% of cells were dead at 43 °C using 3 mg/mL of MNPs and applying a magnetic field of 530 kHz with 20 kA/m amplitude. |
| first_indexed | 2024-03-10T00:50:01Z |
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| id | doaj.art-73ecc40f2cb84ca4b1a479147c7381ec |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T00:50:01Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-73ecc40f2cb84ca4b1a479147c7381ec2023-11-23T14:53:32ZengMDPI AGMolecules1420-30492022-01-0127254410.3390/molecules27020544A Ferrofluid with Surface Modified Nanoparticles for Magnetic Hyperthermia and High ROS ProductionOscar Cervantes0Zaira del Rocio Lopez1Norberto Casillas2Peter Knauth3Nayeli Checa4Francisco Apolinar Cholico5Rodolfo Hernandez-Gutiérrez6Luis Hector Quintero7Jose Avila Paz8Mario Eduardo Cano9Centro Universitario de Ciencias Exactas e Ingenierías, Universidad de Guadalajara, Marcelino García Barragán 1421, Col. Olímpica, Guadalajara C.P. 44430, Jalisco, MexicoCentro Universitario de la Ciénega, Universidad de Guadalajara, Av. Universidad 1115, Col. Linda Vista, Ocotlan C.P. 47810, Jalisco, MexicoCentro Universitario de Ciencias Exactas e Ingenierías, Universidad de Guadalajara, Marcelino García Barragán 1421, Col. Olímpica, Guadalajara C.P. 44430, Jalisco, MexicoCentro Universitario de la Ciénega, Universidad de Guadalajara, Av. Universidad 1115, Col. Linda Vista, Ocotlan C.P. 47810, Jalisco, MexicoCentro Universitario de la Ciénega, Universidad de Guadalajara, Av. Universidad 1115, Col. Linda Vista, Ocotlan C.P. 47810, Jalisco, MexicoCentro Universitario de la Ciénega, Universidad de Guadalajara, Av. Universidad 1115, Col. Linda Vista, Ocotlan C.P. 47810, Jalisco, MexicoCentro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, A.C. Av. Normalistas 800 Colinas de La Normal, Guadalajara C.P. 44270, Jalisco, MexicoCentro Universitario de Ciencias Económico Administrativas, Universidad de Guadalajara, Periférico Norte 799, Col. Los Belenes, Zapopan C.P. 45100, Jalisco, MexicoCentro Universitario de la Ciénega, Universidad de Guadalajara, Av. Universidad 1115, Col. Linda Vista, Ocotlan C.P. 47810, Jalisco, MexicoCentro Universitario de la Ciénega, Universidad de Guadalajara, Av. Universidad 1115, Col. Linda Vista, Ocotlan C.P. 47810, Jalisco, MexicoA ferrofluid with 1,2-Benzenediol-coated iron oxide nanoparticles was synthesized and physicochemically analyzed. This colloidal system was prepared following the typical co-precipitation method, and superparamagnetic nanoparticles of 13.5 nm average diameter, 34 emu/g of magnetic saturation, and 285 K of blocking temperature were obtained. Additionally, the zeta potential showed a suitable colloidal stability for cancer therapy assays and the magneto-calorimetric trails determined a high power absorption density. In addition, the oxidative capability of the ferrofluid was corroborated by performing the Fenton reaction with methylene blue (MB) dissolved in water, where the ferrofluid was suitable for producing reactive oxygen species (ROS), and surprisingly a strong degradation of MB was also observed when it was combined with H<sub>2</sub>O<sub>2</sub>. The intracellular ROS production was qualitatively corroborated using the HT-29 human cell line, by detecting the fluorescent rise induced in 2,7-dichlorofluorescein diacetate. In other experiments, cell metabolic activity was measured, and no toxicity was observed, even with concentrations of up to 4 mg/mL of magnetic nanoparticles (MNPs). When the cells were treated with magnetic hyperthermia, 80% of cells were dead at 43 °C using 3 mg/mL of MNPs and applying a magnetic field of 530 kHz with 20 kA/m amplitude.https://www.mdpi.com/1420-3049/27/2/544superparamagnetismhyperthermiaferrofluidcolloidal-stabilityROS |
| spellingShingle | Oscar Cervantes Zaira del Rocio Lopez Norberto Casillas Peter Knauth Nayeli Checa Francisco Apolinar Cholico Rodolfo Hernandez-Gutiérrez Luis Hector Quintero Jose Avila Paz Mario Eduardo Cano A Ferrofluid with Surface Modified Nanoparticles for Magnetic Hyperthermia and High ROS Production Molecules superparamagnetism hyperthermia ferrofluid colloidal-stability ROS |
| title | A Ferrofluid with Surface Modified Nanoparticles for Magnetic Hyperthermia and High ROS Production |
| title_full | A Ferrofluid with Surface Modified Nanoparticles for Magnetic Hyperthermia and High ROS Production |
| title_fullStr | A Ferrofluid with Surface Modified Nanoparticles for Magnetic Hyperthermia and High ROS Production |
| title_full_unstemmed | A Ferrofluid with Surface Modified Nanoparticles for Magnetic Hyperthermia and High ROS Production |
| title_short | A Ferrofluid with Surface Modified Nanoparticles for Magnetic Hyperthermia and High ROS Production |
| title_sort | ferrofluid with surface modified nanoparticles for magnetic hyperthermia and high ros production |
| topic | superparamagnetism hyperthermia ferrofluid colloidal-stability ROS |
| url | https://www.mdpi.com/1420-3049/27/2/544 |
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