Generation of a Sprague-Dawley-GFP rat iPS cell line
We generated a rat iPSC line called ATCi-rSD95 from transgenic Sprague-Dawley GFP fetal fibroblasts. Established ATCi-rSD95 cells present a normal karyotype, silencing of the transgenes and express pluripotency-associated markers. Additionally, ATCi-rSD95 cells are able to form teratoma with differe...
Main Authors: | , , , , , , , |
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Format: | Article |
Language: | English |
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Elsevier
2017-05-01
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Series: | Stem Cell Research |
Online Access: | http://www.sciencedirect.com/science/article/pii/S187350611730065X |
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author | Giulia Coppiello Gloria Abizanda Natalia Aguado Elena Iglesias Olalla Iglesias-Garcia Antonio Lo Nigro Felipe Prosper Xabier L. Aranguren |
author_facet | Giulia Coppiello Gloria Abizanda Natalia Aguado Elena Iglesias Olalla Iglesias-Garcia Antonio Lo Nigro Felipe Prosper Xabier L. Aranguren |
author_sort | Giulia Coppiello |
collection | DOAJ |
description | We generated a rat iPSC line called ATCi-rSD95 from transgenic Sprague-Dawley GFP fetal fibroblasts. Established ATCi-rSD95 cells present a normal karyotype, silencing of the transgenes and express pluripotency-associated markers. Additionally, ATCi-rSD95 cells are able to form teratoma with differentiated cells derived from the three germ-layers that maintain the GFP expression. |
first_indexed | 2024-12-13T22:10:54Z |
format | Article |
id | doaj.art-73eed17e5cc04da1821105c9d04d7bef |
institution | Directory Open Access Journal |
issn | 1873-5061 1876-7753 |
language | English |
last_indexed | 2024-12-13T22:10:54Z |
publishDate | 2017-05-01 |
publisher | Elsevier |
record_format | Article |
series | Stem Cell Research |
spelling | doaj.art-73eed17e5cc04da1821105c9d04d7bef2022-12-21T23:29:43ZengElsevierStem Cell Research1873-50611876-77532017-05-0121C475010.1016/j.scr.2017.03.021Generation of a Sprague-Dawley-GFP rat iPS cell lineGiulia Coppiello0Gloria Abizanda1Natalia Aguado2Elena Iglesias3Olalla Iglesias-Garcia4Antonio Lo Nigro5Felipe Prosper6Xabier L. Aranguren7Cell Therapy Program, Center for Applied Medical Research (CIMA), University of Navarra, Health Research Institute of Navarra, Pamplona, SpainCell Therapy Program, Center for Applied Medical Research (CIMA), University of Navarra, Health Research Institute of Navarra, Pamplona, SpainCell Therapy Program, Center for Applied Medical Research (CIMA), University of Navarra, Health Research Institute of Navarra, Pamplona, SpainCell Therapy Program, Center for Applied Medical Research (CIMA), University of Navarra, Health Research Institute of Navarra, Pamplona, SpainCenter of Regenerative Medicine in Barcelona (CMRB), SpainRi.Med Foundation, Department of Laboratory Medicine and Advanced Biotechnologies, IRCCS-ISMETT (Istituto Mediterraneo per i Trapianti e Terapie ad Alta Specializzazione), Palermo, ItalyCell Therapy Program, Center for Applied Medical Research (CIMA), University of Navarra, Health Research Institute of Navarra, Pamplona, SpainCell Therapy Program, Center for Applied Medical Research (CIMA), University of Navarra, Health Research Institute of Navarra, Pamplona, SpainWe generated a rat iPSC line called ATCi-rSD95 from transgenic Sprague-Dawley GFP fetal fibroblasts. Established ATCi-rSD95 cells present a normal karyotype, silencing of the transgenes and express pluripotency-associated markers. Additionally, ATCi-rSD95 cells are able to form teratoma with differentiated cells derived from the three germ-layers that maintain the GFP expression.http://www.sciencedirect.com/science/article/pii/S187350611730065X |
spellingShingle | Giulia Coppiello Gloria Abizanda Natalia Aguado Elena Iglesias Olalla Iglesias-Garcia Antonio Lo Nigro Felipe Prosper Xabier L. Aranguren Generation of a Sprague-Dawley-GFP rat iPS cell line Stem Cell Research |
title | Generation of a Sprague-Dawley-GFP rat iPS cell line |
title_full | Generation of a Sprague-Dawley-GFP rat iPS cell line |
title_fullStr | Generation of a Sprague-Dawley-GFP rat iPS cell line |
title_full_unstemmed | Generation of a Sprague-Dawley-GFP rat iPS cell line |
title_short | Generation of a Sprague-Dawley-GFP rat iPS cell line |
title_sort | generation of a sprague dawley gfp rat ips cell line |
url | http://www.sciencedirect.com/science/article/pii/S187350611730065X |
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