Generation of a Sprague-Dawley-GFP rat iPS cell line

We generated a rat iPSC line called ATCi-rSD95 from transgenic Sprague-Dawley GFP fetal fibroblasts. Established ATCi-rSD95 cells present a normal karyotype, silencing of the transgenes and express pluripotency-associated markers. Additionally, ATCi-rSD95 cells are able to form teratoma with differe...

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Main Authors: Giulia Coppiello, Gloria Abizanda, Natalia Aguado, Elena Iglesias, Olalla Iglesias-Garcia, Antonio Lo Nigro, Felipe Prosper, Xabier L. Aranguren
Format: Article
Language:English
Published: Elsevier 2017-05-01
Series:Stem Cell Research
Online Access:http://www.sciencedirect.com/science/article/pii/S187350611730065X
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author Giulia Coppiello
Gloria Abizanda
Natalia Aguado
Elena Iglesias
Olalla Iglesias-Garcia
Antonio Lo Nigro
Felipe Prosper
Xabier L. Aranguren
author_facet Giulia Coppiello
Gloria Abizanda
Natalia Aguado
Elena Iglesias
Olalla Iglesias-Garcia
Antonio Lo Nigro
Felipe Prosper
Xabier L. Aranguren
author_sort Giulia Coppiello
collection DOAJ
description We generated a rat iPSC line called ATCi-rSD95 from transgenic Sprague-Dawley GFP fetal fibroblasts. Established ATCi-rSD95 cells present a normal karyotype, silencing of the transgenes and express pluripotency-associated markers. Additionally, ATCi-rSD95 cells are able to form teratoma with differentiated cells derived from the three germ-layers that maintain the GFP expression.
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spelling doaj.art-73eed17e5cc04da1821105c9d04d7bef2022-12-21T23:29:43ZengElsevierStem Cell Research1873-50611876-77532017-05-0121C475010.1016/j.scr.2017.03.021Generation of a Sprague-Dawley-GFP rat iPS cell lineGiulia Coppiello0Gloria Abizanda1Natalia Aguado2Elena Iglesias3Olalla Iglesias-Garcia4Antonio Lo Nigro5Felipe Prosper6Xabier L. Aranguren7Cell Therapy Program, Center for Applied Medical Research (CIMA), University of Navarra, Health Research Institute of Navarra, Pamplona, SpainCell Therapy Program, Center for Applied Medical Research (CIMA), University of Navarra, Health Research Institute of Navarra, Pamplona, SpainCell Therapy Program, Center for Applied Medical Research (CIMA), University of Navarra, Health Research Institute of Navarra, Pamplona, SpainCell Therapy Program, Center for Applied Medical Research (CIMA), University of Navarra, Health Research Institute of Navarra, Pamplona, SpainCenter of Regenerative Medicine in Barcelona (CMRB), SpainRi.Med Foundation, Department of Laboratory Medicine and Advanced Biotechnologies, IRCCS-ISMETT (Istituto Mediterraneo per i Trapianti e Terapie ad Alta Specializzazione), Palermo, ItalyCell Therapy Program, Center for Applied Medical Research (CIMA), University of Navarra, Health Research Institute of Navarra, Pamplona, SpainCell Therapy Program, Center for Applied Medical Research (CIMA), University of Navarra, Health Research Institute of Navarra, Pamplona, SpainWe generated a rat iPSC line called ATCi-rSD95 from transgenic Sprague-Dawley GFP fetal fibroblasts. Established ATCi-rSD95 cells present a normal karyotype, silencing of the transgenes and express pluripotency-associated markers. Additionally, ATCi-rSD95 cells are able to form teratoma with differentiated cells derived from the three germ-layers that maintain the GFP expression.http://www.sciencedirect.com/science/article/pii/S187350611730065X
spellingShingle Giulia Coppiello
Gloria Abizanda
Natalia Aguado
Elena Iglesias
Olalla Iglesias-Garcia
Antonio Lo Nigro
Felipe Prosper
Xabier L. Aranguren
Generation of a Sprague-Dawley-GFP rat iPS cell line
Stem Cell Research
title Generation of a Sprague-Dawley-GFP rat iPS cell line
title_full Generation of a Sprague-Dawley-GFP rat iPS cell line
title_fullStr Generation of a Sprague-Dawley-GFP rat iPS cell line
title_full_unstemmed Generation of a Sprague-Dawley-GFP rat iPS cell line
title_short Generation of a Sprague-Dawley-GFP rat iPS cell line
title_sort generation of a sprague dawley gfp rat ips cell line
url http://www.sciencedirect.com/science/article/pii/S187350611730065X
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