Application of Fixed-Length Ultrasonic Interferometry to Determine the Kinetics of Light-/Heat-Induced Damage to Biological Membranes and Protein Complexes
This manuscript describes the application of a fixed-length ultrasonic spectrometer to determine the kinetics of heat- and photo-induced damage to biological membranes and protein complexes and provides examples of the test measurements. We implemented a measurement scheme using the digital analysis...
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MDPI AG
2022-09-01
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| Series: | Inventions |
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| Online Access: | https://www.mdpi.com/2411-5134/7/4/87 |
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| author | Denis V. Yanykin Maxim E. Astashev Andrey A. Khorobrykh Mark O. Paskhin Dmitriy A. Serov Sergey V. Gudkov |
| author_facet | Denis V. Yanykin Maxim E. Astashev Andrey A. Khorobrykh Mark O. Paskhin Dmitriy A. Serov Sergey V. Gudkov |
| author_sort | Denis V. Yanykin |
| collection | DOAJ |
| description | This manuscript describes the application of a fixed-length ultrasonic spectrometer to determine the kinetics of heat- and photo-induced damage to biological membranes and protein complexes and provides examples of the test measurements. We implemented a measurement scheme using the digital analysis of harmonic signals. To conduct the research, the fixed-length ultrasonic spectrometer was modernized: the speed was increased; lighting was supplied to the sample cells; the possibility of changing the gas atmosphere and mixing the sample was given. Using solutions containing natural concentrations of deuterium oxide, a high sensitivity of the spectrometer was shown. The spectrometer performed well in the measurement of phase state of dimyristoylphosphatidylcholine liposomes, both in the absence and in the presence of additions, which are capable of changing the lipid properties (sodium dodecyl sulfate, palmitic acid, and calcium ions). The heat- and photo-induced changes in the state of photosystem II core complexes were demonstrated using a fixed-length ultrasonic spectrometer. Transitions at 35.5 °C, 43.5 °C, 56.5 °C, and 66.7 °C were revealed. It is proposed that the transitions reflect the disassembly of the complexes and protein denaturation. Thus, the present study demonstrates that a fixed-length ultrasonic spectrometer can be applied to determine the kinetics of heat- and photo-induced damage to biological membranes and protein complexes. |
| first_indexed | 2024-03-09T09:45:49Z |
| format | Article |
| id | doaj.art-73f1317a3a7d425ba73941277805f8c7 |
| institution | Directory Open Access Journal |
| issn | 2411-5134 |
| language | English |
| last_indexed | 2024-03-09T09:45:49Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Inventions |
| spelling | doaj.art-73f1317a3a7d425ba73941277805f8c72023-12-02T00:37:02ZengMDPI AGInventions2411-51342022-09-01748710.3390/inventions7040087Application of Fixed-Length Ultrasonic Interferometry to Determine the Kinetics of Light-/Heat-Induced Damage to Biological Membranes and Protein ComplexesDenis V. Yanykin0Maxim E. Astashev1Andrey A. Khorobrykh2Mark O. Paskhin3Dmitriy A. Serov4Sergey V. Gudkov5Prokhorov General Physics Institute of the Russian Academy of Sciences, 38 Vavilova St., 119991 Moscow, RussiaProkhorov General Physics Institute of the Russian Academy of Sciences, 38 Vavilova St., 119991 Moscow, RussiaInstitute of Basic Biological Problems, FRC PSCBR, Russian Academy of Sciences, 2 Institutskaya St., Pushchino, 142290 Moscow, RussiaProkhorov General Physics Institute of the Russian Academy of Sciences, 38 Vavilova St., 119991 Moscow, RussiaProkhorov General Physics Institute of the Russian Academy of Sciences, 38 Vavilova St., 119991 Moscow, RussiaProkhorov General Physics Institute of the Russian Academy of Sciences, 38 Vavilova St., 119991 Moscow, RussiaThis manuscript describes the application of a fixed-length ultrasonic spectrometer to determine the kinetics of heat- and photo-induced damage to biological membranes and protein complexes and provides examples of the test measurements. We implemented a measurement scheme using the digital analysis of harmonic signals. To conduct the research, the fixed-length ultrasonic spectrometer was modernized: the speed was increased; lighting was supplied to the sample cells; the possibility of changing the gas atmosphere and mixing the sample was given. Using solutions containing natural concentrations of deuterium oxide, a high sensitivity of the spectrometer was shown. The spectrometer performed well in the measurement of phase state of dimyristoylphosphatidylcholine liposomes, both in the absence and in the presence of additions, which are capable of changing the lipid properties (sodium dodecyl sulfate, palmitic acid, and calcium ions). The heat- and photo-induced changes in the state of photosystem II core complexes were demonstrated using a fixed-length ultrasonic spectrometer. Transitions at 35.5 °C, 43.5 °C, 56.5 °C, and 66.7 °C were revealed. It is proposed that the transitions reflect the disassembly of the complexes and protein denaturation. Thus, the present study demonstrates that a fixed-length ultrasonic spectrometer can be applied to determine the kinetics of heat- and photo-induced damage to biological membranes and protein complexes.https://www.mdpi.com/2411-5134/7/4/87ultrasoundfixed-length ultrasonic spectrometerbiological membranesprotein complexesphase statesphase transitions |
| spellingShingle | Denis V. Yanykin Maxim E. Astashev Andrey A. Khorobrykh Mark O. Paskhin Dmitriy A. Serov Sergey V. Gudkov Application of Fixed-Length Ultrasonic Interferometry to Determine the Kinetics of Light-/Heat-Induced Damage to Biological Membranes and Protein Complexes Inventions ultrasound fixed-length ultrasonic spectrometer biological membranes protein complexes phase states phase transitions |
| title | Application of Fixed-Length Ultrasonic Interferometry to Determine the Kinetics of Light-/Heat-Induced Damage to Biological Membranes and Protein Complexes |
| title_full | Application of Fixed-Length Ultrasonic Interferometry to Determine the Kinetics of Light-/Heat-Induced Damage to Biological Membranes and Protein Complexes |
| title_fullStr | Application of Fixed-Length Ultrasonic Interferometry to Determine the Kinetics of Light-/Heat-Induced Damage to Biological Membranes and Protein Complexes |
| title_full_unstemmed | Application of Fixed-Length Ultrasonic Interferometry to Determine the Kinetics of Light-/Heat-Induced Damage to Biological Membranes and Protein Complexes |
| title_short | Application of Fixed-Length Ultrasonic Interferometry to Determine the Kinetics of Light-/Heat-Induced Damage to Biological Membranes and Protein Complexes |
| title_sort | application of fixed length ultrasonic interferometry to determine the kinetics of light heat induced damage to biological membranes and protein complexes |
| topic | ultrasound fixed-length ultrasonic spectrometer biological membranes protein complexes phase states phase transitions |
| url | https://www.mdpi.com/2411-5134/7/4/87 |
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