235 Analysiss of TNBC Cell Lines Cultured a Novel Translational Breast Cancer Microphysiological System (BC-MPS)
OBJECTIVES/GOALS: Current approaches to drug development for the aggressive triple negative breast cancer rely on current 2D and 3D in vitro models which have limited capabilities. We have developed a translational microphysiological system that can maintain the human breast microenvironment to capt...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Cambridge University Press
2022-04-01
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| Series: | Journal of Clinical and Translational Science |
| Online Access: | https://www.cambridge.org/core/product/identifier/S2059866122001248/type/journal_article |
| _version_ | 1811155287408640000 |
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| author | Katherine L. Hebert Khoa Nguyen Thomas Cheng Madlin Alzoubi Steven Elliott Bridgette Collins-Burow Elizabeth Martin Frank Lau Matthew Burow |
| author_facet | Katherine L. Hebert Khoa Nguyen Thomas Cheng Madlin Alzoubi Steven Elliott Bridgette Collins-Burow Elizabeth Martin Frank Lau Matthew Burow |
| author_sort | Katherine L. Hebert |
| collection | DOAJ |
| description | OBJECTIVES/GOALS: Current approaches to drug development for the aggressive triple negative breast cancer rely on current 2D and 3D in vitro models which have limited capabilities. We have developed a translational microphysiological system that can maintain the human breast microenvironment to capture the complex interaction with the tumor microenvironment. METHODS/STUDY POPULATION: Three different TNBC cell lines were seeded in BC-MPS: MDA-MB-231 parental cell line, MDA-MB-231wiht the gene, LKB1 overexpressed, which is a tumor suppressor, and MDA-MB-231 with the enzyme, ERK5, an enzyme associated with increased metastasis and drug resistance, knocked out. These three TNBC cell lines were cultured in a standard 2D 96-well plate and in BC-MPS. Time-lapse videos were taken to track cellular mobility. RNA-sequencing was performed to compare different expression levels of various cancer related genes of the cell lines cultured in standard 2D and BC-MPS. RESULTS/ANTICIPATED RESULTS: The LKB1 overexpressed MDA-MB-231 and the ERK5-ko MDA-MB-231 cell lines are expected to have decreased mobility compared to the parental cells. The cell lines are expected to have increased expression of cancer related genes when cultured in BC-MPS than when cultured in standard 2D due to the presence of human breast tissue. DISCUSSION/SIGNIFICANCE: BC-MPS is a promising new translational MPS that facilitates studying long term interactions between real human breast tissue and cancer cells. The BC-MPS systems ability to support the growth of established cell lines has been demonstrated. Future studies will focus on developing the model for personalized medicine. |
| first_indexed | 2024-04-10T04:30:35Z |
| format | Article |
| id | doaj.art-73f54d70a90d4f1fbb317857a216fee9 |
| institution | Directory Open Access Journal |
| issn | 2059-8661 |
| language | English |
| last_indexed | 2024-04-10T04:30:35Z |
| publishDate | 2022-04-01 |
| publisher | Cambridge University Press |
| record_format | Article |
| series | Journal of Clinical and Translational Science |
| spelling | doaj.art-73f54d70a90d4f1fbb317857a216fee92023-03-10T07:53:48ZengCambridge University PressJournal of Clinical and Translational Science2059-86612022-04-016373710.1017/cts.2022.124235 Analysiss of TNBC Cell Lines Cultured a Novel Translational Breast Cancer Microphysiological System (BC-MPS)Katherine L. Hebert0Khoa Nguyen1Thomas Cheng2Madlin Alzoubi3Steven Elliott4Bridgette Collins-Burow5Elizabeth Martin6Frank Lau7Matthew Burow8Tulane UniversityTulane UniversityTulane UniversityTulane UniversityTulane UniversityTulane UniversityLouisiana State UniversityLouisiana State University Health Sciences CenterTulane UniversityOBJECTIVES/GOALS: Current approaches to drug development for the aggressive triple negative breast cancer rely on current 2D and 3D in vitro models which have limited capabilities. We have developed a translational microphysiological system that can maintain the human breast microenvironment to capture the complex interaction with the tumor microenvironment. METHODS/STUDY POPULATION: Three different TNBC cell lines were seeded in BC-MPS: MDA-MB-231 parental cell line, MDA-MB-231wiht the gene, LKB1 overexpressed, which is a tumor suppressor, and MDA-MB-231 with the enzyme, ERK5, an enzyme associated with increased metastasis and drug resistance, knocked out. These three TNBC cell lines were cultured in a standard 2D 96-well plate and in BC-MPS. Time-lapse videos were taken to track cellular mobility. RNA-sequencing was performed to compare different expression levels of various cancer related genes of the cell lines cultured in standard 2D and BC-MPS. RESULTS/ANTICIPATED RESULTS: The LKB1 overexpressed MDA-MB-231 and the ERK5-ko MDA-MB-231 cell lines are expected to have decreased mobility compared to the parental cells. The cell lines are expected to have increased expression of cancer related genes when cultured in BC-MPS than when cultured in standard 2D due to the presence of human breast tissue. DISCUSSION/SIGNIFICANCE: BC-MPS is a promising new translational MPS that facilitates studying long term interactions between real human breast tissue and cancer cells. The BC-MPS systems ability to support the growth of established cell lines has been demonstrated. Future studies will focus on developing the model for personalized medicine.https://www.cambridge.org/core/product/identifier/S2059866122001248/type/journal_article |
| spellingShingle | Katherine L. Hebert Khoa Nguyen Thomas Cheng Madlin Alzoubi Steven Elliott Bridgette Collins-Burow Elizabeth Martin Frank Lau Matthew Burow 235 Analysiss of TNBC Cell Lines Cultured a Novel Translational Breast Cancer Microphysiological System (BC-MPS) Journal of Clinical and Translational Science |
| title | 235 Analysiss of TNBC Cell Lines Cultured a Novel Translational Breast Cancer Microphysiological System (BC-MPS) |
| title_full | 235 Analysiss of TNBC Cell Lines Cultured a Novel Translational Breast Cancer Microphysiological System (BC-MPS) |
| title_fullStr | 235 Analysiss of TNBC Cell Lines Cultured a Novel Translational Breast Cancer Microphysiological System (BC-MPS) |
| title_full_unstemmed | 235 Analysiss of TNBC Cell Lines Cultured a Novel Translational Breast Cancer Microphysiological System (BC-MPS) |
| title_short | 235 Analysiss of TNBC Cell Lines Cultured a Novel Translational Breast Cancer Microphysiological System (BC-MPS) |
| title_sort | 235 analysiss of tnbc cell lines cultured a novel translational breast cancer microphysiological system bc mps |
| url | https://www.cambridge.org/core/product/identifier/S2059866122001248/type/journal_article |
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