Tissue clearing may alter emission and absorption properties of common fluorophores

Abstract In recent years, 3D cell culture has been gaining a more widespread following across many fields of biology. Tissue clearing enables optical analysis of intact 3D samples and investigation of molecular and structural mechanisms by homogenizing the refractive indices of tissues to make them...

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Bibliographic Details
Main Authors: Farsam Eliat, Rebecca Sohn, Henrik Renner, Theresa Kagermeier, Stefan Volkery, Heike Brinkmann, Nils Kirschnick, Friedemann Kiefer, Martha Grabos, Katharina Becker, Ivan Bedzhov, Hans R. Schöler, Jan M. Bruder
Format: Article
Language:English
Published: Nature Portfolio 2022-04-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-022-09303-9