Hydrocarbons Biodegradation by <i>Rhodococcus</i>: Assimilation of Hexadecane in Different Aggregate States
The aim of our study was to reveal the peculiarities of the adaptation of rhodococci to hydrophobic hydrocarbon degradation at low temperatures when the substrate was in solid states. The ability of actinobacteria <i>Rhodococcus erythropolis</i> (strains X5 and S67) to degrade hexadecane...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-08-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/10/8/1594 |
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| author | Luong Thi Mo Puntus Irina Suzina Natalia Nechaeva Irina Akhmetov Lenar Filonov Andrey Akatova Ekaterina Alferov Sergey Ponamoreva Olga |
| author_facet | Luong Thi Mo Puntus Irina Suzina Natalia Nechaeva Irina Akhmetov Lenar Filonov Andrey Akatova Ekaterina Alferov Sergey Ponamoreva Olga |
| author_sort | Luong Thi Mo |
| collection | DOAJ |
| description | The aim of our study was to reveal the peculiarities of the adaptation of rhodococci to hydrophobic hydrocarbon degradation at low temperatures when the substrate was in solid states. The ability of actinobacteria <i>Rhodococcus erythropolis</i> (strains X5 and S67) to degrade hexadecane at 10 °C (solid hydrophobic substrate) and 26 °C (liquid hydrophobic substrate) is described. Despite the solid state of the hydrophobic substrate at 10 °C, bacteria demonstrate a high level of its degradation (30–40%) within 18 days. For the first time, we show that specialized cellular structures are formed during the degradation of solid hexadecane by <i>Rhodococcus</i> at low temperatures: intracellular multimembrane structures and surface vesicles connected to the cell by fibers. The formation of specialized cellular structures when <i>Rhodococcus</i> bacteria are grown on solid hexadecane is an important adaptive trait, thereby contributing to the enlargement of a contact area between membrane-bound enzymes and a hydrophobic substrate. |
| first_indexed | 2024-03-09T04:03:37Z |
| format | Article |
| id | doaj.art-73facba171b44e3f98ee6a0b4184838d |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T04:03:37Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-73facba171b44e3f98ee6a0b4184838d2023-12-03T14:09:25ZengMDPI AGMicroorganisms2076-26072022-08-01108159410.3390/microorganisms10081594Hydrocarbons Biodegradation by <i>Rhodococcus</i>: Assimilation of Hexadecane in Different Aggregate StatesLuong Thi Mo0Puntus Irina1Suzina Natalia2Nechaeva Irina3Akhmetov Lenar4Filonov Andrey5Akatova Ekaterina6Alferov Sergey7Ponamoreva Olga8Department of Biotechnology, Tula State University, Prospekt Lenina 92, 300012 Tula, RussiaLaboratory of Plasmid Biology, Skryabin Institute of Biochemistry and Physiology of Microorganisms of Russian Academy of Sciences—A Separate Subdivision of Federal State Budget Institution of Science, Federal Research Centre, Pushchino Scientific Center of Biological Research of Russian Academy of Sciences, Prospekt Nauki 5, 142290 Pushchino, RussiaLaboratory of Microbial Cytology, Skryabin Institute of Biochemistry and Physiology of Microorganisms of Russian Academy of Sciences—A Separate Subdivision of Federal State Budget Institution of Science, Federal Research Centre, Pushchino Scientific Center of Biological Research of Russian Academy of Sciences, Prospekt Nauki 5, 142290 Pushchino, RussiaLaboratory of Ecological and Medical Biotechnology, Tula State University, Friedrich Engels Street 157, 300012 Tula, RussiaLaboratory of Plasmid Biology, Skryabin Institute of Biochemistry and Physiology of Microorganisms of Russian Academy of Sciences—A Separate Subdivision of Federal State Budget Institution of Science, Federal Research Centre, Pushchino Scientific Center of Biological Research of Russian Academy of Sciences, Prospekt Nauki 5, 142290 Pushchino, RussiaLaboratory of Plasmid Biology, Skryabin Institute of Biochemistry and Physiology of Microorganisms of Russian Academy of Sciences—A Separate Subdivision of Federal State Budget Institution of Science, Federal Research Centre, Pushchino Scientific Center of Biological Research of Russian Academy of Sciences, Prospekt Nauki 5, 142290 Pushchino, RussiaLaboratory of Ecological and Medical Biotechnology, Tula State University, Friedrich Engels Street 157, 300012 Tula, RussiaLaboratory of Ecological and Medical Biotechnology, Tula State University, Friedrich Engels Street 157, 300012 Tula, RussiaDepartment of Biotechnology, Tula State University, Prospekt Lenina 92, 300012 Tula, RussiaThe aim of our study was to reveal the peculiarities of the adaptation of rhodococci to hydrophobic hydrocarbon degradation at low temperatures when the substrate was in solid states. The ability of actinobacteria <i>Rhodococcus erythropolis</i> (strains X5 and S67) to degrade hexadecane at 10 °C (solid hydrophobic substrate) and 26 °C (liquid hydrophobic substrate) is described. Despite the solid state of the hydrophobic substrate at 10 °C, bacteria demonstrate a high level of its degradation (30–40%) within 18 days. For the first time, we show that specialized cellular structures are formed during the degradation of solid hexadecane by <i>Rhodococcus</i> at low temperatures: intracellular multimembrane structures and surface vesicles connected to the cell by fibers. The formation of specialized cellular structures when <i>Rhodococcus</i> bacteria are grown on solid hexadecane is an important adaptive trait, thereby contributing to the enlargement of a contact area between membrane-bound enzymes and a hydrophobic substrate.https://www.mdpi.com/2076-2607/10/8/1594hydrocarbon biodegradation<i>Rhodococcus</i>biosurfactantssolid and liquid substratehexadecaneadaptation |
| spellingShingle | Luong Thi Mo Puntus Irina Suzina Natalia Nechaeva Irina Akhmetov Lenar Filonov Andrey Akatova Ekaterina Alferov Sergey Ponamoreva Olga Hydrocarbons Biodegradation by <i>Rhodococcus</i>: Assimilation of Hexadecane in Different Aggregate States Microorganisms hydrocarbon biodegradation <i>Rhodococcus</i> biosurfactants solid and liquid substrate hexadecane adaptation |
| title | Hydrocarbons Biodegradation by <i>Rhodococcus</i>: Assimilation of Hexadecane in Different Aggregate States |
| title_full | Hydrocarbons Biodegradation by <i>Rhodococcus</i>: Assimilation of Hexadecane in Different Aggregate States |
| title_fullStr | Hydrocarbons Biodegradation by <i>Rhodococcus</i>: Assimilation of Hexadecane in Different Aggregate States |
| title_full_unstemmed | Hydrocarbons Biodegradation by <i>Rhodococcus</i>: Assimilation of Hexadecane in Different Aggregate States |
| title_short | Hydrocarbons Biodegradation by <i>Rhodococcus</i>: Assimilation of Hexadecane in Different Aggregate States |
| title_sort | hydrocarbons biodegradation by i rhodococcus i assimilation of hexadecane in different aggregate states |
| topic | hydrocarbon biodegradation <i>Rhodococcus</i> biosurfactants solid and liquid substrate hexadecane adaptation |
| url | https://www.mdpi.com/2076-2607/10/8/1594 |
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