Upregulated Long Non-Coding RNA LL22NC03-N64E9.1 Promotes the Proliferation and Migration of Human Breast Cancer Cells by Silencing Kruppel-Like Factor 2 Expression
Weibin Lian,1,* Xiaohua Jiang,2,* Liangqiang Li,1,* Qinglan Wang,1 Chengye Hong,1 Peidong Yang,1 Debo Chen1 1Department of Breast Surgery, Quanzhou First Hospital Affiliated to Fujian Medical University, Quanzhou, Fujian, People’s Republic of China; 2Department of Orthopedics, Xiang&rs...
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Dove Medical Press
2020-10-01
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| Series: | Cancer Management and Research |
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| Online Access: | https://www.dovepress.com/upregulated-long-non-coding-rna-ll22nc03-n64e91-promotes-the-prolifera-peer-reviewed-article-CMAR |
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| author | Lian W Jiang X Li L Wang Q Hong C Yang P Chen D |
| author_facet | Lian W Jiang X Li L Wang Q Hong C Yang P Chen D |
| author_sort | Lian W |
| collection | DOAJ |
| description | Weibin Lian,1,* Xiaohua Jiang,2,* Liangqiang Li,1,* Qinglan Wang,1 Chengye Hong,1 Peidong Yang,1 Debo Chen1 1Department of Breast Surgery, Quanzhou First Hospital Affiliated to Fujian Medical University, Quanzhou, Fujian, People’s Republic of China; 2Department of Orthopedics, Xiang’an Hospital of Xiamen University, School of Medicine, Xiamen University, Xiamen, Fujian, People’s Republic of China*These authors contributed equally to this workCorrespondence: Weibin Lian; Debo Chen Tel +86-15260819892; +86-13600738668Email weibinlian@fjmu.edu.cn; deboqz@163.comIntroduction: Recently, the significant regulatory effects of lncRNAs on the oncogenesis and growth of tumor have been demonstrated by an increasing number of research projects. A previous study showed that LL22NC03-N64E9.1 could promote the development of colorectal cancer, especially via enhanced cell proliferation. Similarly, this lncRNA should have comparable functions in breast cancer (BC), which requires in-depth investigation. Therefore, this study was designed to explore the correlation of LL22NC03-N64E9.1 with BC.Methods: qRT-PCR was used to assess the relative expression of LL22NC03-N64E9.1 in BC tissues. Cell viability examination and colony formation experiments were performed to investigate the role of LL22NC03-N64E9.1 in BC cell’s proliferation. Transwell assays were used to explore the effects of LL22NC03-N64E9.1 on BC cell’s migration. RNA immunoprecipitation, chromosome immunoprecipitation assay and rescue experiments were performed to analyze the association of LL22NC03-N64E9.1 with target proteins and genes in BC cells.Results: We identified that LL22NC03-N64E9.1 is an oncogene, upregulated in BC, which was verified in a cohort of 48 pairs of BC tissues. Based on the loss-of-function experiments, silencing LL22NC03-N64E9.1 expression significantly inhibited malignancy progression. In terms of the mechanism, LL22NC03-N64E9.1 acted on the enhancer of zeste homolog 2 (EZH2) by direct binding, which promoted BC cell growth. Furthermore, in the promoters of KLF2, the trimethylation of H3K27 could be regulated by LL22NC03-N64E9.1 as the mediator.Conclusion: Relying on the LL22NC03-N64E9.1/EZH2/KLF2 pathway, the lncRNA LL22NC03-N64E9.1 was significantly associated with BC development and could, therefore, be a potential therapeutic target to block BC growth.Keywords: breast cancer, lncRNA, LL22NC03-N64E9.1, H3K27me3, KLF2 |
| first_indexed | 2024-12-20T01:46:54Z |
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| language | English |
| last_indexed | 2024-12-20T01:46:54Z |
| publishDate | 2020-10-01 |
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| series | Cancer Management and Research |
| spelling | doaj.art-73fd2790387b40d89d7e81ac80bae0ce2022-12-21T19:57:44ZengDove Medical PressCancer Management and Research1179-13222020-10-01Volume 12107631077058677Upregulated Long Non-Coding RNA LL22NC03-N64E9.1 Promotes the Proliferation and Migration of Human Breast Cancer Cells by Silencing Kruppel-Like Factor 2 ExpressionLian WJiang XLi LWang QHong CYang PChen DWeibin Lian,1,* Xiaohua Jiang,2,* Liangqiang Li,1,* Qinglan Wang,1 Chengye Hong,1 Peidong Yang,1 Debo Chen1 1Department of Breast Surgery, Quanzhou First Hospital Affiliated to Fujian Medical University, Quanzhou, Fujian, People’s Republic of China; 2Department of Orthopedics, Xiang’an Hospital of Xiamen University, School of Medicine, Xiamen University, Xiamen, Fujian, People’s Republic of China*These authors contributed equally to this workCorrespondence: Weibin Lian; Debo Chen Tel +86-15260819892; +86-13600738668Email weibinlian@fjmu.edu.cn; deboqz@163.comIntroduction: Recently, the significant regulatory effects of lncRNAs on the oncogenesis and growth of tumor have been demonstrated by an increasing number of research projects. A previous study showed that LL22NC03-N64E9.1 could promote the development of colorectal cancer, especially via enhanced cell proliferation. Similarly, this lncRNA should have comparable functions in breast cancer (BC), which requires in-depth investigation. Therefore, this study was designed to explore the correlation of LL22NC03-N64E9.1 with BC.Methods: qRT-PCR was used to assess the relative expression of LL22NC03-N64E9.1 in BC tissues. Cell viability examination and colony formation experiments were performed to investigate the role of LL22NC03-N64E9.1 in BC cell’s proliferation. Transwell assays were used to explore the effects of LL22NC03-N64E9.1 on BC cell’s migration. RNA immunoprecipitation, chromosome immunoprecipitation assay and rescue experiments were performed to analyze the association of LL22NC03-N64E9.1 with target proteins and genes in BC cells.Results: We identified that LL22NC03-N64E9.1 is an oncogene, upregulated in BC, which was verified in a cohort of 48 pairs of BC tissues. Based on the loss-of-function experiments, silencing LL22NC03-N64E9.1 expression significantly inhibited malignancy progression. In terms of the mechanism, LL22NC03-N64E9.1 acted on the enhancer of zeste homolog 2 (EZH2) by direct binding, which promoted BC cell growth. Furthermore, in the promoters of KLF2, the trimethylation of H3K27 could be regulated by LL22NC03-N64E9.1 as the mediator.Conclusion: Relying on the LL22NC03-N64E9.1/EZH2/KLF2 pathway, the lncRNA LL22NC03-N64E9.1 was significantly associated with BC development and could, therefore, be a potential therapeutic target to block BC growth.Keywords: breast cancer, lncRNA, LL22NC03-N64E9.1, H3K27me3, KLF2https://www.dovepress.com/upregulated-long-non-coding-rna-ll22nc03-n64e91-promotes-the-prolifera-peer-reviewed-article-CMARbreast cancerlncrnall22nc03-n64e9.1h3k27me3klf2 |
| spellingShingle | Lian W Jiang X Li L Wang Q Hong C Yang P Chen D Upregulated Long Non-Coding RNA LL22NC03-N64E9.1 Promotes the Proliferation and Migration of Human Breast Cancer Cells by Silencing Kruppel-Like Factor 2 Expression Cancer Management and Research breast cancer lncrna ll22nc03-n64e9.1 h3k27me3 klf2 |
| title | Upregulated Long Non-Coding RNA LL22NC03-N64E9.1 Promotes the Proliferation and Migration of Human Breast Cancer Cells by Silencing Kruppel-Like Factor 2 Expression |
| title_full | Upregulated Long Non-Coding RNA LL22NC03-N64E9.1 Promotes the Proliferation and Migration of Human Breast Cancer Cells by Silencing Kruppel-Like Factor 2 Expression |
| title_fullStr | Upregulated Long Non-Coding RNA LL22NC03-N64E9.1 Promotes the Proliferation and Migration of Human Breast Cancer Cells by Silencing Kruppel-Like Factor 2 Expression |
| title_full_unstemmed | Upregulated Long Non-Coding RNA LL22NC03-N64E9.1 Promotes the Proliferation and Migration of Human Breast Cancer Cells by Silencing Kruppel-Like Factor 2 Expression |
| title_short | Upregulated Long Non-Coding RNA LL22NC03-N64E9.1 Promotes the Proliferation and Migration of Human Breast Cancer Cells by Silencing Kruppel-Like Factor 2 Expression |
| title_sort | upregulated long non coding rna ll22nc03 n64e9 1 promotes the proliferation and migration of human breast cancer cells by silencing kruppel like factor 2 expression |
| topic | breast cancer lncrna ll22nc03-n64e9.1 h3k27me3 klf2 |
| url | https://www.dovepress.com/upregulated-long-non-coding-rna-ll22nc03-n64e91-promotes-the-prolifera-peer-reviewed-article-CMAR |
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