Effect of LncRNA LINC00857 Knockdown on Migration, Proliferation and Apoptosis of Pancreatic Cancer PANC-1 Cells
Objective To investigate the expression of LncRNA LINC00857 in pancreatic cells and the effect of lncRNA LINC00857 down-regulation on proliferation, migration and apoptosis of pancreatic cancer PANC-1 cells and possible mechanism. Methods The lentiviral vector GV112 was constructed and infected PANC...
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| Format: | Article |
| Language: | zho |
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Magazine House of Cancer Research on Prevention and Treatment
2021-02-01
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| Series: | Zhongliu Fangzhi Yanjiu |
| Subjects: | |
| Online Access: | http://html.rhhz.net/ZLFZYJ/html/8578.2021.20.0882.htm |
| _version_ | 1818741949506519040 |
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| author | ZHANG Bo REN Longfei HU Jinjing BAI Zhongtian ZHOU Wence |
| author_facet | ZHANG Bo REN Longfei HU Jinjing BAI Zhongtian ZHOU Wence |
| author_sort | ZHANG Bo |
| collection | DOAJ |
| description | Objective To investigate the expression of LncRNA LINC00857 in pancreatic cells and the effect of lncRNA LINC00857 down-regulation on proliferation, migration and apoptosis of pancreatic cancer PANC-1 cells and possible mechanism. Methods The lentiviral vector GV112 was constructed and infected PANC-1 cells to obtain experimental group, while the blank plasmid was transfected as a negative control group and the cells without intervention were taken as normal control group. CCK-8 assay, Transwell assay, scratch test, flow cytometry, Western blot were used to detect the effect of LINC00857 down-regulation on cell proliferation, migration, apoptosis, cell cycle and EMT-related proteins. Results LINC00857 expression in pancreatic cancer cells were significantly higher than that in normal pancreatic epithelial cells (P < 0.001). Knockdown of LINC00857 could significantly inhibit the proliferation and migration of PANC-1 cells (P < 0.0001); compared with the negative control group, the apoptosis rates of cells in the experimental groups were significantly increased (P < 0.05), the number of cells in G0/G1 phase increased (P < 0.01), the number of cells in S phase decreased (P < 0.05), and the cells were blocked in the G1 phase, E-cadherin expression was significantly up-regulated (P < 0.05) and N-cadherin and Vimentin expression were significantly down-regulated (N-cadherin: P < 0.01, Vimentin: P < 0.05). Conclusion LINC00857 can promote proliferation and migration of PANC-1 cells and inhibit its apoptosis by regulating G1/S phase transition and EMT signaling pathway. |
| first_indexed | 2024-12-18T02:04:45Z |
| format | Article |
| id | doaj.art-73ff19a4371f4585a68168693ba09eb3 |
| institution | Directory Open Access Journal |
| issn | 1000-8578 1000-8578 |
| language | zho |
| last_indexed | 2024-12-18T02:04:45Z |
| publishDate | 2021-02-01 |
| publisher | Magazine House of Cancer Research on Prevention and Treatment |
| record_format | Article |
| series | Zhongliu Fangzhi Yanjiu |
| spelling | doaj.art-73ff19a4371f4585a68168693ba09eb32022-12-21T21:24:38ZzhoMagazine House of Cancer Research on Prevention and TreatmentZhongliu Fangzhi Yanjiu1000-85781000-85782021-02-0148212713210.3971/j.issn.1000-8578.2021.20.08828578.2021.20.0882Effect of LncRNA LINC00857 Knockdown on Migration, Proliferation and Apoptosis of Pancreatic Cancer PANC-1 CellsZHANG Bo0REN Longfei1HU Jinjing2BAI Zhongtian3ZHOU Wence4The First Clinical Medical College, Lanzhou University, Lanzhou 730000, ChinaDepartment of General Surgery, The First Hospital of Lanzhou University, Lanzhou 730000, ChinaGansu Province Key Laboratory of Biotherapy and Regenerative Medicine, Lanzhou 730000, ChinaDepartment of General Surgery, The First Hospital of Lanzhou University, Lanzhou 730000, ChinaThe First Clinical Medical College, Lanzhou University, Lanzhou 730000, ChinaObjective To investigate the expression of LncRNA LINC00857 in pancreatic cells and the effect of lncRNA LINC00857 down-regulation on proliferation, migration and apoptosis of pancreatic cancer PANC-1 cells and possible mechanism. Methods The lentiviral vector GV112 was constructed and infected PANC-1 cells to obtain experimental group, while the blank plasmid was transfected as a negative control group and the cells without intervention were taken as normal control group. CCK-8 assay, Transwell assay, scratch test, flow cytometry, Western blot were used to detect the effect of LINC00857 down-regulation on cell proliferation, migration, apoptosis, cell cycle and EMT-related proteins. Results LINC00857 expression in pancreatic cancer cells were significantly higher than that in normal pancreatic epithelial cells (P < 0.001). Knockdown of LINC00857 could significantly inhibit the proliferation and migration of PANC-1 cells (P < 0.0001); compared with the negative control group, the apoptosis rates of cells in the experimental groups were significantly increased (P < 0.05), the number of cells in G0/G1 phase increased (P < 0.01), the number of cells in S phase decreased (P < 0.05), and the cells were blocked in the G1 phase, E-cadherin expression was significantly up-regulated (P < 0.05) and N-cadherin and Vimentin expression were significantly down-regulated (N-cadherin: P < 0.01, Vimentin: P < 0.05). Conclusion LINC00857 can promote proliferation and migration of PANC-1 cells and inhibit its apoptosis by regulating G1/S phase transition and EMT signaling pathway.http://html.rhhz.net/ZLFZYJ/html/8578.2021.20.0882.htmpancreatic cancerlncrna linc00857emtapoptosiscell cycleproliferationmigration |
| spellingShingle | ZHANG Bo REN Longfei HU Jinjing BAI Zhongtian ZHOU Wence Effect of LncRNA LINC00857 Knockdown on Migration, Proliferation and Apoptosis of Pancreatic Cancer PANC-1 Cells Zhongliu Fangzhi Yanjiu pancreatic cancer lncrna linc00857 emt apoptosis cell cycle proliferation migration |
| title | Effect of LncRNA LINC00857 Knockdown on Migration, Proliferation and Apoptosis of Pancreatic Cancer PANC-1 Cells |
| title_full | Effect of LncRNA LINC00857 Knockdown on Migration, Proliferation and Apoptosis of Pancreatic Cancer PANC-1 Cells |
| title_fullStr | Effect of LncRNA LINC00857 Knockdown on Migration, Proliferation and Apoptosis of Pancreatic Cancer PANC-1 Cells |
| title_full_unstemmed | Effect of LncRNA LINC00857 Knockdown on Migration, Proliferation and Apoptosis of Pancreatic Cancer PANC-1 Cells |
| title_short | Effect of LncRNA LINC00857 Knockdown on Migration, Proliferation and Apoptosis of Pancreatic Cancer PANC-1 Cells |
| title_sort | effect of lncrna linc00857 knockdown on migration proliferation and apoptosis of pancreatic cancer panc 1 cells |
| topic | pancreatic cancer lncrna linc00857 emt apoptosis cell cycle proliferation migration |
| url | http://html.rhhz.net/ZLFZYJ/html/8578.2021.20.0882.htm |
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