Pectinase Production by Rhizopus stolonifer A3 Isolated from Apple Peels
The application of pectinase in industries will continue to be developed. To broaden the applicability of enzyme manufacturing in new industries, more research to investigate pectinolytic microorganisms with high activity and stability is still required. Therefore, this study aimed to obtain pectino...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Universitas Sebelas Maret, Faculty of Agriculture
2023-11-01
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| Series: | Caraka Tani: Journal of Sustainable Agriculture |
| Subjects: | |
| Online Access: | https://jurnal.uns.ac.id/carakatani/article/view/77610 |
| _version_ | 1797234113079410688 |
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| author | Siti Lusi Arum Sari Wida Puspa Tanjung Klara Rizky Amilia Ratna Setyaningsih Artini Pangastuti |
| author_facet | Siti Lusi Arum Sari Wida Puspa Tanjung Klara Rizky Amilia Ratna Setyaningsih Artini Pangastuti |
| author_sort | Siti Lusi Arum Sari |
| collection | DOAJ |
| description | The application of pectinase in industries will continue to be developed. To broaden the applicability of enzyme manufacturing in new industries, more research to investigate pectinolytic microorganisms with high activity and stability is still required. Therefore, this study aimed to obtain pectinolytic fungi that have the potential as pectinase producers. Pectinolytic fungi were isolated from rotten apple peels and selected based on clear zone formation on pectic agar media after cetyl trimethyl ammonium bromide (CTAB) staining. Pectinolytic fungi were identified based on 18S rRNA partial gene sequences and morphological characteristics. Pectinase production used Mandels and Weber medium with citrus pectin 20 g l-1 addition. Pectinase activity was determined based on the measurement of reducing sugars by a colorimetric method. A total of 5 fungal isolates were successfully isolated. All of the isolates had pectinolytic activities with clear zone diameters ranging between 0.99 to 7.32 mm. The isolate A3 showing the highest pectinolytic activity was identified as Rhizopus stolonifer. Microscopically, R. stolonifer A3 showed typical characteristics for Rhizopus, characterized by the presence of rhizoids, stolons and sporangiophores bearing a single spherical sporangium. The pectinase production of R. stolonifer A3 was optimum at initial pH 5.0, temperature 35 °C and incubation period of 3 days with pectinase activity of 14.75 U ml-1. R. stolonifer A3 produced acidic pectinase having optimum activity at pH 5.0 and temperature 50 °C. Thus R. stolonifer A3 has the potential to be used as a producer of acidic pectinase appropriate for use in the processing of fruit products. |
| first_indexed | 2024-04-24T16:26:54Z |
| format | Article |
| id | doaj.art-740070b3560045ea8c665d57887f13f2 |
| institution | Directory Open Access Journal |
| issn | 2613-9456 2599-2570 |
| language | English |
| last_indexed | 2024-04-24T16:26:54Z |
| publishDate | 2023-11-01 |
| publisher | Universitas Sebelas Maret, Faculty of Agriculture |
| record_format | Article |
| series | Caraka Tani: Journal of Sustainable Agriculture |
| spelling | doaj.art-740070b3560045ea8c665d57887f13f22024-03-31T03:27:47ZengUniversitas Sebelas Maret, Faculty of AgricultureCaraka Tani: Journal of Sustainable Agriculture2613-94562599-25702023-11-01391102110.20961/carakatani.v39i1.7761039944Pectinase Production by Rhizopus stolonifer A3 Isolated from Apple PeelsSiti Lusi Arum Sari0Wida Puspa Tanjung1Klara Rizky Amilia2Ratna Setyaningsih3Artini Pangastuti4Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sebelas Maret, SurakartaDepartment of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sebelas Maret, SurakartaDepartment of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sebelas Maret, SurakartaDepartment of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sebelas Maret, SurakartaDepartment of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sebelas Maret, SurakartaThe application of pectinase in industries will continue to be developed. To broaden the applicability of enzyme manufacturing in new industries, more research to investigate pectinolytic microorganisms with high activity and stability is still required. Therefore, this study aimed to obtain pectinolytic fungi that have the potential as pectinase producers. Pectinolytic fungi were isolated from rotten apple peels and selected based on clear zone formation on pectic agar media after cetyl trimethyl ammonium bromide (CTAB) staining. Pectinolytic fungi were identified based on 18S rRNA partial gene sequences and morphological characteristics. Pectinase production used Mandels and Weber medium with citrus pectin 20 g l-1 addition. Pectinase activity was determined based on the measurement of reducing sugars by a colorimetric method. A total of 5 fungal isolates were successfully isolated. All of the isolates had pectinolytic activities with clear zone diameters ranging between 0.99 to 7.32 mm. The isolate A3 showing the highest pectinolytic activity was identified as Rhizopus stolonifer. Microscopically, R. stolonifer A3 showed typical characteristics for Rhizopus, characterized by the presence of rhizoids, stolons and sporangiophores bearing a single spherical sporangium. The pectinase production of R. stolonifer A3 was optimum at initial pH 5.0, temperature 35 °C and incubation period of 3 days with pectinase activity of 14.75 U ml-1. R. stolonifer A3 produced acidic pectinase having optimum activity at pH 5.0 and temperature 50 °C. Thus R. stolonifer A3 has the potential to be used as a producer of acidic pectinase appropriate for use in the processing of fruit products.https://jurnal.uns.ac.id/carakatani/article/view/77610apple peelpectinasepectinolytic fungirhizopus stolonifer |
| spellingShingle | Siti Lusi Arum Sari Wida Puspa Tanjung Klara Rizky Amilia Ratna Setyaningsih Artini Pangastuti Pectinase Production by Rhizopus stolonifer A3 Isolated from Apple Peels Caraka Tani: Journal of Sustainable Agriculture apple peel pectinase pectinolytic fungi rhizopus stolonifer |
| title | Pectinase Production by Rhizopus stolonifer A3 Isolated from Apple Peels |
| title_full | Pectinase Production by Rhizopus stolonifer A3 Isolated from Apple Peels |
| title_fullStr | Pectinase Production by Rhizopus stolonifer A3 Isolated from Apple Peels |
| title_full_unstemmed | Pectinase Production by Rhizopus stolonifer A3 Isolated from Apple Peels |
| title_short | Pectinase Production by Rhizopus stolonifer A3 Isolated from Apple Peels |
| title_sort | pectinase production by rhizopus stolonifer a3 isolated from apple peels |
| topic | apple peel pectinase pectinolytic fungi rhizopus stolonifer |
| url | https://jurnal.uns.ac.id/carakatani/article/view/77610 |
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