Safety assessment of multiple repeated percutaneous punctures for the collection of cerebrospinal fluid in rats
The objective of this study was to examine the safety of multiple repeated percutaneous punctures of cisterna magna for collecting cerebrospinal fluid (CSF) and preliminarily determine the optimal time interval and volume at each collection. Sixty Wistar rats were randomly assigned to six groups: 10...
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| Format: | Article |
| Language: | English |
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Associação Brasileira de Divulgação Científica
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| Series: | Brazilian Journal of Medical and Biological Research |
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| Online Access: | http://www.scielo.br/pdf/bjmbr/v54n6/1414-431X-bjmbr-54-6-e10032.pdf |
| _version_ | 1819004196241801216 |
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| author | Dongxue Wang Ying Zhao Yang Yang Hailong Xie |
| author_facet | Dongxue Wang Ying Zhao Yang Yang Hailong Xie |
| author_sort | Dongxue Wang |
| collection | DOAJ |
| description | The objective of this study was to examine the safety of multiple repeated percutaneous punctures of cisterna magna for collecting cerebrospinal fluid (CSF) and preliminarily determine the optimal time interval and volume at each collection. Sixty Wistar rats were randomly assigned to six groups: 10 d-0 μL, 10 d-100 μL (100 μL CSF collected at an interval of 10 days), 10 d-150 μL, 15 d-0 μL, 15 d-100 μL, and 15 d-150 μL. CSF was collected by percutaneous puncture of the cisterna magna at four time-points. Simultaneously, locomotor activity, cisterna magna pressure, and acetylcholine levels in the CSF were monitored. Compared with the 10 d-0 μL group, the escape latency by Morris water maze was significantly prolonged in the 10 d-100 μL and 10 d-150 μL groups (P<0.05). Compared with the 15 d-0 μL group, the indices of 15 d-100 μL and 15 d-150 μL groups had no significant differences. When compared with that at the first training, the exception of the 10 d-150 μL and 15 d-150 μL groups, significant differences in escape latency were found at the 6th attempt (P<0.05). Compared with baseline readings for each group, the cisterna magna pressure in the 10 d-150 μL group began to decrease significantly from the third measurement (P<0.05). The optimal time interval during four CSF collections (100 μL per collection) via cisterna magna percutaneous puncture was determined to be 15 days. The procedure did not significantly affect learning processes, performance, or other related indices. |
| first_indexed | 2024-12-20T23:33:03Z |
| format | Article |
| id | doaj.art-740462fbe70843949583e5e40b9de62d |
| institution | Directory Open Access Journal |
| issn | 1414-431X |
| language | English |
| last_indexed | 2024-12-20T23:33:03Z |
| publisher | Associação Brasileira de Divulgação Científica |
| record_format | Article |
| series | Brazilian Journal of Medical and Biological Research |
| spelling | doaj.art-740462fbe70843949583e5e40b9de62d2022-12-21T19:23:15ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research1414-431X10.1590/1414-431x202010032Safety assessment of multiple repeated percutaneous punctures for the collection of cerebrospinal fluid in ratsDongxue WangYing ZhaoYang YangHailong XieThe objective of this study was to examine the safety of multiple repeated percutaneous punctures of cisterna magna for collecting cerebrospinal fluid (CSF) and preliminarily determine the optimal time interval and volume at each collection. Sixty Wistar rats were randomly assigned to six groups: 10 d-0 μL, 10 d-100 μL (100 μL CSF collected at an interval of 10 days), 10 d-150 μL, 15 d-0 μL, 15 d-100 μL, and 15 d-150 μL. CSF was collected by percutaneous puncture of the cisterna magna at four time-points. Simultaneously, locomotor activity, cisterna magna pressure, and acetylcholine levels in the CSF were monitored. Compared with the 10 d-0 μL group, the escape latency by Morris water maze was significantly prolonged in the 10 d-100 μL and 10 d-150 μL groups (P<0.05). Compared with the 15 d-0 μL group, the indices of 15 d-100 μL and 15 d-150 μL groups had no significant differences. When compared with that at the first training, the exception of the 10 d-150 μL and 15 d-150 μL groups, significant differences in escape latency were found at the 6th attempt (P<0.05). Compared with baseline readings for each group, the cisterna magna pressure in the 10 d-150 μL group began to decrease significantly from the third measurement (P<0.05). The optimal time interval during four CSF collections (100 μL per collection) via cisterna magna percutaneous puncture was determined to be 15 days. The procedure did not significantly affect learning processes, performance, or other related indices.http://www.scielo.br/pdf/bjmbr/v54n6/1414-431X-bjmbr-54-6-e10032.pdfCerebrospinal fluidSafetyCisterna magnaBrainMemory and learning test |
| spellingShingle | Dongxue Wang Ying Zhao Yang Yang Hailong Xie Safety assessment of multiple repeated percutaneous punctures for the collection of cerebrospinal fluid in rats Brazilian Journal of Medical and Biological Research Cerebrospinal fluid Safety Cisterna magna Brain Memory and learning test |
| title | Safety assessment of multiple repeated percutaneous punctures for the collection of cerebrospinal fluid in rats |
| title_full | Safety assessment of multiple repeated percutaneous punctures for the collection of cerebrospinal fluid in rats |
| title_fullStr | Safety assessment of multiple repeated percutaneous punctures for the collection of cerebrospinal fluid in rats |
| title_full_unstemmed | Safety assessment of multiple repeated percutaneous punctures for the collection of cerebrospinal fluid in rats |
| title_short | Safety assessment of multiple repeated percutaneous punctures for the collection of cerebrospinal fluid in rats |
| title_sort | safety assessment of multiple repeated percutaneous punctures for the collection of cerebrospinal fluid in rats |
| topic | Cerebrospinal fluid Safety Cisterna magna Brain Memory and learning test |
| url | http://www.scielo.br/pdf/bjmbr/v54n6/1414-431X-bjmbr-54-6-e10032.pdf |
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