Chitinase Chi 2 Positively Regulates Cucumber Resistance against <i>Fusarium oxysporum</i> f. sp. cucumerinum
Cucumber (<i>Cucumis sativus</i> L.) is an important vegetable crop worldwide, and <i>Fusarium</i> wilt (<i>FW</i>), caused by <i>Fusarium oxysporum</i> f. sp. cucumerinum (Foc), severely restricts cucumber growth and yield. Accumulating lines of evide...
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MDPI AG
2021-12-01
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| Series: | Genes |
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| author | Jun Xu Ningyuan Zhang Ke Wang Qianqian Xian Jingping Dong Xiaohua Qi Xuehao Chen |
| author_facet | Jun Xu Ningyuan Zhang Ke Wang Qianqian Xian Jingping Dong Xiaohua Qi Xuehao Chen |
| author_sort | Jun Xu |
| collection | DOAJ |
| description | Cucumber (<i>Cucumis sativus</i> L.) is an important vegetable crop worldwide, and <i>Fusarium</i> wilt (<i>FW</i>), caused by <i>Fusarium oxysporum</i> f. sp. cucumerinum (Foc), severely restricts cucumber growth and yield. Accumulating lines of evidence indicate that chitinases play important roles in attacking the invading fungal pathogens through catalyzing their cell wall degradation. Here, we identified the chitinase (Chi) genes in cucumber and further screened the <i>FW</i>-responsive genes via a comparative transcriptome analysis and found that six common genes were predominantly expressed in roots but also significantly upregulated after Foc infection. Expression verification further conformed that <i>Chi2</i> and <i>Chi14</i> were obviously induced by Foc as well as by hormone treatments, compared with the controls. The purified Chi2 and Chi14 proteins significantly affected the growth of Foc in vitro, compared with the controls. Knockdown of <i>Chi2</i> in cucumber by virus-induced gene silencing (VIGS) increased susceptibility to <i>FW</i>, compared with the <i>Chi14</i>-silenced and control plants, and silencing of <i>Chi2</i> drastically impaired gene activation in the jasmonic acid pathway, suggesting that the <i>Chi2</i> gene might play positive roles in cucumber <i>FW</i> defense and, therefore, can provide a gene resource for developing cucumber-<i>FW</i>-resistance breeding programs. |
| first_indexed | 2024-03-10T01:25:55Z |
| format | Article |
| id | doaj.art-74063728e8da46ccb1954dba22c3dc65 |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-10T01:25:55Z |
| publishDate | 2021-12-01 |
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| record_format | Article |
| series | Genes |
| spelling | doaj.art-74063728e8da46ccb1954dba22c3dc652023-11-23T13:51:43ZengMDPI AGGenes2073-44252021-12-011316210.3390/genes13010062Chitinase Chi 2 Positively Regulates Cucumber Resistance against <i>Fusarium oxysporum</i> f. sp. cucumerinumJun Xu0Ningyuan Zhang1Ke Wang2Qianqian Xian3Jingping Dong4Xiaohua Qi5Xuehao Chen6School of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, ChinaSchool of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, ChinaSchool of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, ChinaSchool of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, ChinaSchool of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, ChinaSchool of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, ChinaSchool of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, ChinaCucumber (<i>Cucumis sativus</i> L.) is an important vegetable crop worldwide, and <i>Fusarium</i> wilt (<i>FW</i>), caused by <i>Fusarium oxysporum</i> f. sp. cucumerinum (Foc), severely restricts cucumber growth and yield. Accumulating lines of evidence indicate that chitinases play important roles in attacking the invading fungal pathogens through catalyzing their cell wall degradation. Here, we identified the chitinase (Chi) genes in cucumber and further screened the <i>FW</i>-responsive genes via a comparative transcriptome analysis and found that six common genes were predominantly expressed in roots but also significantly upregulated after Foc infection. Expression verification further conformed that <i>Chi2</i> and <i>Chi14</i> were obviously induced by Foc as well as by hormone treatments, compared with the controls. The purified Chi2 and Chi14 proteins significantly affected the growth of Foc in vitro, compared with the controls. Knockdown of <i>Chi2</i> in cucumber by virus-induced gene silencing (VIGS) increased susceptibility to <i>FW</i>, compared with the <i>Chi14</i>-silenced and control plants, and silencing of <i>Chi2</i> drastically impaired gene activation in the jasmonic acid pathway, suggesting that the <i>Chi2</i> gene might play positive roles in cucumber <i>FW</i> defense and, therefore, can provide a gene resource for developing cucumber-<i>FW</i>-resistance breeding programs.https://www.mdpi.com/2073-4425/13/1/62cucumbertranscriptomechitinases<i>Fusarium</i> wiltresistance |
| spellingShingle | Jun Xu Ningyuan Zhang Ke Wang Qianqian Xian Jingping Dong Xiaohua Qi Xuehao Chen Chitinase Chi 2 Positively Regulates Cucumber Resistance against <i>Fusarium oxysporum</i> f. sp. cucumerinum Genes cucumber transcriptome chitinases <i>Fusarium</i> wilt resistance |
| title | Chitinase Chi 2 Positively Regulates Cucumber Resistance against <i>Fusarium oxysporum</i> f. sp. cucumerinum |
| title_full | Chitinase Chi 2 Positively Regulates Cucumber Resistance against <i>Fusarium oxysporum</i> f. sp. cucumerinum |
| title_fullStr | Chitinase Chi 2 Positively Regulates Cucumber Resistance against <i>Fusarium oxysporum</i> f. sp. cucumerinum |
| title_full_unstemmed | Chitinase Chi 2 Positively Regulates Cucumber Resistance against <i>Fusarium oxysporum</i> f. sp. cucumerinum |
| title_short | Chitinase Chi 2 Positively Regulates Cucumber Resistance against <i>Fusarium oxysporum</i> f. sp. cucumerinum |
| title_sort | chitinase chi 2 positively regulates cucumber resistance against i fusarium oxysporum i f sp cucumerinum |
| topic | cucumber transcriptome chitinases <i>Fusarium</i> wilt resistance |
| url | https://www.mdpi.com/2073-4425/13/1/62 |
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