<i>Linum lewisii</i> Adventitious and Hairy-Roots Cultures as Lignan Plant Factories
Plants synthesize specific secondary metabolites for survival, reproduction, environmental resilience, and defense. Among them, lignans are a class of polyphenols with several bioactive properties: chemopreventive, anti-inflammatory, antiviral, and antioxidant. These compounds are often extracted fr...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-08-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/11/8/1526 |
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| author | Roméo Arago Dougué Kentsop Roberto Consonni Michela Alfieri Marina Laura Gianluca Ottolina Iride Mascheretti Monica Mattana |
| author_facet | Roméo Arago Dougué Kentsop Roberto Consonni Michela Alfieri Marina Laura Gianluca Ottolina Iride Mascheretti Monica Mattana |
| author_sort | Roméo Arago Dougué Kentsop |
| collection | DOAJ |
| description | Plants synthesize specific secondary metabolites for survival, reproduction, environmental resilience, and defense. Among them, lignans are a class of polyphenols with several bioactive properties: chemopreventive, anti-inflammatory, antiviral, and antioxidant. These compounds are often extracted from field-grown plants with very low yields. To overcome these constraints, in vitro tissue cultures provide a tool to optimize large-scale production. Moreover, the use of elicitation to increase secondary metabolite production is gaining importance. The aim of this work was to develop adventitious (ARL) and hairy roots (HRL) from <i>Linum lewisi</i>, a species able to synthesize arylnaphthalene lignans such as justicidin B. The ARL and HRL were obtained for the first time and characterized for their phenol content, antioxidant activity, and the production of justicidin B after treatments with several elicitors and precursor feeding. Through NMR spectroscopy, other four lignans were highlighted and identified in the roots extracts. A pilot-scale bioreactor was adopted to assess the suitability of the developed root cultures for future large-scale production. The ARL and HRL cultures showed a justicidin B production higher than other <i>Linum</i> species cultures described up to now (75.8 mg/L and 82.2 g/L), and the production more than doubled after elicitation with MeJA. |
| first_indexed | 2024-03-09T11:58:33Z |
| format | Article |
| id | doaj.art-74074037d87f46aaa72f6b5dfc9c4d2e |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-09T11:58:33Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-74074037d87f46aaa72f6b5dfc9c4d2e2023-11-30T23:06:09ZengMDPI AGAntioxidants2076-39212022-08-01118152610.3390/antiox11081526<i>Linum lewisii</i> Adventitious and Hairy-Roots Cultures as Lignan Plant FactoriesRoméo Arago Dougué Kentsop0Roberto Consonni1Michela Alfieri2Marina Laura3Gianluca Ottolina4Iride Mascheretti5Monica Mattana6Institute of Agricultural Biology and Biotechnology, National Research Council, Via Bassini 15, 20133 Milan, ItalyInstitute of Chemical Sciences and Technologies “Giulio Natta”, National Research Council, Via Corti 12, 20133 Milan, ItalyInstitute of Chemical Sciences and Technologies “Giulio Natta”, National Research Council, Via Corti 12, 20133 Milan, ItalyCREA Research Centre for Vegetable and Ornamental Crops (CREA OF), Corso degli Inglesi 508, 18038 Sanremo, ItalyInstitute of Chemical Sciences and Technologies “Giulio Natta”, National Research Council, Via Corti 12, 20133 Milan, ItalyInstitute of Agricultural Biology and Biotechnology, National Research Council, Via Bassini 15, 20133 Milan, ItalyInstitute of Agricultural Biology and Biotechnology, National Research Council, Via Bassini 15, 20133 Milan, ItalyPlants synthesize specific secondary metabolites for survival, reproduction, environmental resilience, and defense. Among them, lignans are a class of polyphenols with several bioactive properties: chemopreventive, anti-inflammatory, antiviral, and antioxidant. These compounds are often extracted from field-grown plants with very low yields. To overcome these constraints, in vitro tissue cultures provide a tool to optimize large-scale production. Moreover, the use of elicitation to increase secondary metabolite production is gaining importance. The aim of this work was to develop adventitious (ARL) and hairy roots (HRL) from <i>Linum lewisi</i>, a species able to synthesize arylnaphthalene lignans such as justicidin B. The ARL and HRL were obtained for the first time and characterized for their phenol content, antioxidant activity, and the production of justicidin B after treatments with several elicitors and precursor feeding. Through NMR spectroscopy, other four lignans were highlighted and identified in the roots extracts. A pilot-scale bioreactor was adopted to assess the suitability of the developed root cultures for future large-scale production. The ARL and HRL cultures showed a justicidin B production higher than other <i>Linum</i> species cultures described up to now (75.8 mg/L and 82.2 g/L), and the production more than doubled after elicitation with MeJA.https://www.mdpi.com/2076-3921/11/8/1526antioxidant capacityelicitationflaxjusticidin Blignansprecursor feeding |
| spellingShingle | Roméo Arago Dougué Kentsop Roberto Consonni Michela Alfieri Marina Laura Gianluca Ottolina Iride Mascheretti Monica Mattana <i>Linum lewisii</i> Adventitious and Hairy-Roots Cultures as Lignan Plant Factories Antioxidants antioxidant capacity elicitation flax justicidin B lignans precursor feeding |
| title | <i>Linum lewisii</i> Adventitious and Hairy-Roots Cultures as Lignan Plant Factories |
| title_full | <i>Linum lewisii</i> Adventitious and Hairy-Roots Cultures as Lignan Plant Factories |
| title_fullStr | <i>Linum lewisii</i> Adventitious and Hairy-Roots Cultures as Lignan Plant Factories |
| title_full_unstemmed | <i>Linum lewisii</i> Adventitious and Hairy-Roots Cultures as Lignan Plant Factories |
| title_short | <i>Linum lewisii</i> Adventitious and Hairy-Roots Cultures as Lignan Plant Factories |
| title_sort | i linum lewisii i adventitious and hairy roots cultures as lignan plant factories |
| topic | antioxidant capacity elicitation flax justicidin B lignans precursor feeding |
| url | https://www.mdpi.com/2076-3921/11/8/1526 |
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