REDERIVATION BY EMBRYO TRANSFER IN STRAINS OF LABORATORY MICE AND RATS
Rederivation allows laboratory animal populations to be purged from specified pathogens and thus turns these animals to the SPF (specified pathogens free) status. Results of the rederivation of two unique rat strains selected at the Institute of Cytology and Genetics and one mouse strain are present...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders
2014-12-01
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| Series: | Вавиловский журнал генетики и селекции |
| Subjects: | |
| Online Access: | https://vavilov.elpub.ru/jour/article/view/121 |
| _version_ | 1797214226399363072 |
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| author | S. Ya. Amstislavsky T. N. Igonina I. N. Rozhkova E. Yu. Brusentsev A. A. Rogovaya D. S. Ragaeva V. A. Naprimerov E. A. Litvinova I. Z. Plyusnina A. L. Markel |
| author_facet | S. Ya. Amstislavsky T. N. Igonina I. N. Rozhkova E. Yu. Brusentsev A. A. Rogovaya D. S. Ragaeva V. A. Naprimerov E. A. Litvinova I. Z. Plyusnina A. L. Markel |
| author_sort | S. Ya. Amstislavsky |
| collection | DOAJ |
| description | Rederivation allows laboratory animal populations to be purged from specified pathogens and thus turns these animals to the SPF (specified pathogens free) status. Results of the rederivation of two unique rat strains selected at the Institute of Cytology and Genetics and one mouse strain are presented. The two rat strains are: tame Norway rats and rats with Inherited Stress Induced Arterial Hypertension (ISIAH strain). The ICR mouse strain has been named as abbreviation of the Institute of Cancer Research wherefrom these mice were distributed to laboratories all over the world. The SPF status of the rats after rederivation was confirmed by the method of indicator animals (sentinel animals). The optimized model of rederivation offered here involves a combination of such embryotechnological methods as freezing/cryopreservation of embryos, their washing through the number of fresh volumes of sterile media, growing in vitro for 48 hours, and subsequent transfer into either one or both uterine horns of recipient females. Application of this model to rederivation of ICR mice yielded 39 pups born in an SPF vivarium. It should be noticed that the effectiveness of the procedure met international standards, and characteristic features of phenotype were retained in all the three strains after rederivation. |
| first_indexed | 2024-03-07T16:08:09Z |
| format | Article |
| id | doaj.art-740ac4646a8741529ec9d56573686569 |
| institution | Directory Open Access Journal |
| issn | 2500-3259 |
| language | English |
| last_indexed | 2024-04-24T11:10:48Z |
| publishDate | 2014-12-01 |
| publisher | Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders |
| record_format | Article |
| series | Вавиловский журнал генетики и селекции |
| spelling | doaj.art-740ac4646a8741529ec9d565736865692024-04-11T15:30:53ZengSiberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and BreedersВавиловский журнал генетики и селекции2500-32592014-12-01171147161105REDERIVATION BY EMBRYO TRANSFER IN STRAINS OF LABORATORY MICE AND RATSS. Ya. Amstislavsky0T. N. Igonina1I. N. Rozhkova2E. Yu. Brusentsev3A. A. Rogovaya4D. S. Ragaeva5V. A. Naprimerov6E. A. Litvinova7I. Z. Plyusnina8A. L. Markel9Institute of Cytology and Genetics SB RAS, Novosibirsk, Russia Novosibirsk National Research State University, Novosibirsk, RussiaInstitute of Cytology and Genetics SB RAS, Novosibirsk, RussiaInstitute of Cytology and Genetics SB RAS, Novosibirsk, RussiaInstitute of Cytology and Genetics SB RAS, Novosibirsk, RussiaNovosibirsk National Research State University, Novosibirsk, RussiaNovosibirsk National Research State University, Novosibirsk, RussiaInstitute of Cytology and Genetics SB RAS, Novosibirsk, Russia State Higher Education Institution Novosibirsk State Agrarian University, Novosibirsk, RussiaInstitute of Cytology and Genetics SB RAS, Novosibirsk, RussiaInstitute of Cytology and Genetics SB RAS, Novosibirsk, Russia Novosibirsk National Research State University, Novosibirsk, RussiaInstitute of Cytology and Genetics SB RAS, Novosibirsk, Russia Novosibirsk National Research State University, Novosibirsk, RussiaRederivation allows laboratory animal populations to be purged from specified pathogens and thus turns these animals to the SPF (specified pathogens free) status. Results of the rederivation of two unique rat strains selected at the Institute of Cytology and Genetics and one mouse strain are presented. The two rat strains are: tame Norway rats and rats with Inherited Stress Induced Arterial Hypertension (ISIAH strain). The ICR mouse strain has been named as abbreviation of the Institute of Cancer Research wherefrom these mice were distributed to laboratories all over the world. The SPF status of the rats after rederivation was confirmed by the method of indicator animals (sentinel animals). The optimized model of rederivation offered here involves a combination of such embryotechnological methods as freezing/cryopreservation of embryos, their washing through the number of fresh volumes of sterile media, growing in vitro for 48 hours, and subsequent transfer into either one or both uterine horns of recipient females. Application of this model to rederivation of ICR mice yielded 39 pups born in an SPF vivarium. It should be noticed that the effectiveness of the procedure met international standards, and characteristic features of phenotype were retained in all the three strains after rederivation.https://vavilov.elpub.ru/jour/article/view/121isiah ratstame ratsicr miceembryo transferrederivation |
| spellingShingle | S. Ya. Amstislavsky T. N. Igonina I. N. Rozhkova E. Yu. Brusentsev A. A. Rogovaya D. S. Ragaeva V. A. Naprimerov E. A. Litvinova I. Z. Plyusnina A. L. Markel REDERIVATION BY EMBRYO TRANSFER IN STRAINS OF LABORATORY MICE AND RATS Вавиловский журнал генетики и селекции isiah rats tame rats icr mice embryo transfer rederivation |
| title | REDERIVATION BY EMBRYO TRANSFER IN STRAINS OF LABORATORY MICE AND RATS |
| title_full | REDERIVATION BY EMBRYO TRANSFER IN STRAINS OF LABORATORY MICE AND RATS |
| title_fullStr | REDERIVATION BY EMBRYO TRANSFER IN STRAINS OF LABORATORY MICE AND RATS |
| title_full_unstemmed | REDERIVATION BY EMBRYO TRANSFER IN STRAINS OF LABORATORY MICE AND RATS |
| title_short | REDERIVATION BY EMBRYO TRANSFER IN STRAINS OF LABORATORY MICE AND RATS |
| title_sort | rederivation by embryo transfer in strains of laboratory mice and rats |
| topic | isiah rats tame rats icr mice embryo transfer rederivation |
| url | https://vavilov.elpub.ru/jour/article/view/121 |
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