Staphylococcus aureus and Salted Fish: Prevalence, Antibiogram, and Detection of Enterotoxin-coding Genes
This study was taken to investigate the prevalence of Staphylococcus aureus (S. aureus) in four types of salted fish, namely salted sardine, fesiekh, sahlia, and salted herrings that retailed in Zagazig city, Egypt. The antimicrobial susceptibility testing of the recovered S. aureus isolates was ex...
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| Format: | Article |
| Language: | English |
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Assiut University
2022-11-01
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| Series: | Journal of Advanced Veterinary Research |
| Online Access: | https://advetresearch.com/index.php/AVR/article/view/1097 |
| _version_ | 1797657052643852288 |
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| author | Alaa Eldin M.A. Morshdy Hassan Mohieldeen Ahmed E. Tharwat Maha Moustafa Rehab E. Mohamed Walaa Fathy SaadEldin Wageh Sobhy Darwish |
| author_facet | Alaa Eldin M.A. Morshdy Hassan Mohieldeen Ahmed E. Tharwat Maha Moustafa Rehab E. Mohamed Walaa Fathy SaadEldin Wageh Sobhy Darwish |
| author_sort | Alaa Eldin M.A. Morshdy |
| collection | DOAJ |
| description |
This study was taken to investigate the prevalence of Staphylococcus aureus (S. aureus) in four types of salted fish, namely salted sardine, fesiekh, sahlia, and salted herrings that retailed in Zagazig city, Egypt. The antimicrobial susceptibility testing of the recovered S. aureus isolates was examined. Moreover, PCR was used for the detection of the coding genes of S. aureus-enterotoxins (SE) including SEA, SEB, SEC, and SED. The obtained results of the current investigation revealed isolation of S. aureus from the examined salted sardine, fesiekh, sahlia, and salted herrings at 15%, 40%, 30%, and 15%, respectively. Fesiekh had significantly (p< 0.05) the highest total S. aureus count (3.17 ± 0.13 log 10 cfu/g), followed by sahlia (3.08 ± 0.13 log 10 cfu/g), sardine (2.33 ± 0.07 log 10 cfu/mL), and salted herrings (2.30 ± 0.11 log 10 cfu/g), respectively. Besides, 10%, 35%, 20%, and 5% of the examined salted sardine, fesiekh, sahlia, and salted herrings, respectively exceeded Egyptian limits of S. aureus. The recovered S. aureus isolates showed clear multidrug resistance profiling. PCR testing of selected S. aureus isolates for harboring Staphylococcal enterotoxin-coding genes revealed SEA, and SEB were not detected in any examined isolate. However, SEC was detected in 3 S. aureus isolates recovered from fesiekh, and in 2 isolates that recovered from sahlia. SED was only detected in 2 isolates that recovered from fesiekh. Therefore, strict hygienic measures should be adopted during handling, and processing of salted fish.
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| first_indexed | 2024-03-11T17:38:48Z |
| format | Article |
| id | doaj.art-74113a7125ec48838091482df53b14c6 |
| institution | Directory Open Access Journal |
| issn | 2090-6269 2090-6277 |
| language | English |
| last_indexed | 2024-03-11T17:38:48Z |
| publishDate | 2022-11-01 |
| publisher | Assiut University |
| record_format | Article |
| series | Journal of Advanced Veterinary Research |
| spelling | doaj.art-74113a7125ec48838091482df53b14c62023-10-18T14:22:45ZengAssiut UniversityJournal of Advanced Veterinary Research2090-62692090-62772022-11-01126Staphylococcus aureus and Salted Fish: Prevalence, Antibiogram, and Detection of Enterotoxin-coding GenesAlaa Eldin M.A. Morshdy0Hassan Mohieldeen1Ahmed E. Tharwat2Maha Moustafa3Rehab E. Mohamed4Walaa Fathy SaadEldin5Wageh Sobhy Darwish6Food Control Department, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44519, Egypt.Food Control Department, Animal Health Research Institute, Zagazig Province Laboratory, Zagazig, Egypt.Food Control Department, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44519, Egypt.Food Control Department, Animal Health Research Institute, Zagazig Province Laboratory, Zagazig, Egypt.Department of Zoonoses, Faculty of Veterinary Medicine, Zagazig University 44519, Egypt.Educational Veterinary Hospital, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44519, Egypt.Food Control Department, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44519, Egypt. This study was taken to investigate the prevalence of Staphylococcus aureus (S. aureus) in four types of salted fish, namely salted sardine, fesiekh, sahlia, and salted herrings that retailed in Zagazig city, Egypt. The antimicrobial susceptibility testing of the recovered S. aureus isolates was examined. Moreover, PCR was used for the detection of the coding genes of S. aureus-enterotoxins (SE) including SEA, SEB, SEC, and SED. The obtained results of the current investigation revealed isolation of S. aureus from the examined salted sardine, fesiekh, sahlia, and salted herrings at 15%, 40%, 30%, and 15%, respectively. Fesiekh had significantly (p< 0.05) the highest total S. aureus count (3.17 ± 0.13 log 10 cfu/g), followed by sahlia (3.08 ± 0.13 log 10 cfu/g), sardine (2.33 ± 0.07 log 10 cfu/mL), and salted herrings (2.30 ± 0.11 log 10 cfu/g), respectively. Besides, 10%, 35%, 20%, and 5% of the examined salted sardine, fesiekh, sahlia, and salted herrings, respectively exceeded Egyptian limits of S. aureus. The recovered S. aureus isolates showed clear multidrug resistance profiling. PCR testing of selected S. aureus isolates for harboring Staphylococcal enterotoxin-coding genes revealed SEA, and SEB were not detected in any examined isolate. However, SEC was detected in 3 S. aureus isolates recovered from fesiekh, and in 2 isolates that recovered from sahlia. SED was only detected in 2 isolates that recovered from fesiekh. Therefore, strict hygienic measures should be adopted during handling, and processing of salted fish. https://advetresearch.com/index.php/AVR/article/view/1097 |
| spellingShingle | Alaa Eldin M.A. Morshdy Hassan Mohieldeen Ahmed E. Tharwat Maha Moustafa Rehab E. Mohamed Walaa Fathy SaadEldin Wageh Sobhy Darwish Staphylococcus aureus and Salted Fish: Prevalence, Antibiogram, and Detection of Enterotoxin-coding Genes Journal of Advanced Veterinary Research |
| title | Staphylococcus aureus and Salted Fish: Prevalence, Antibiogram, and Detection of Enterotoxin-coding Genes |
| title_full | Staphylococcus aureus and Salted Fish: Prevalence, Antibiogram, and Detection of Enterotoxin-coding Genes |
| title_fullStr | Staphylococcus aureus and Salted Fish: Prevalence, Antibiogram, and Detection of Enterotoxin-coding Genes |
| title_full_unstemmed | Staphylococcus aureus and Salted Fish: Prevalence, Antibiogram, and Detection of Enterotoxin-coding Genes |
| title_short | Staphylococcus aureus and Salted Fish: Prevalence, Antibiogram, and Detection of Enterotoxin-coding Genes |
| title_sort | staphylococcus aureus and salted fish prevalence antibiogram and detection of enterotoxin coding genes |
| url | https://advetresearch.com/index.php/AVR/article/view/1097 |
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