Formulation and candidacidal activity of magnetic nanoparticles coated with cathelicidin LL-37 and ceragenin CSA-13
Abstract Fungal infections caused by Candida spp. represent an emerging problem during treatment of immunocompromised patients and those hospitalized with serious principal diseases. The ever-growing number of fungal strains exhibiting drug resistance necessitates the development of novel antimicrob...
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| Format: | Article |
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Nature Portfolio
2017-07-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-017-04653-1 |
| _version_ | 1818991430456049664 |
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| author | Katarzyna Niemirowicz Bonita Durnaś Grażyna Tokajuk Ewelina Piktel Grzegorz Michalak Xiaobo Gu Alina Kułakowska Paul B. Savage Robert Bucki |
| author_facet | Katarzyna Niemirowicz Bonita Durnaś Grażyna Tokajuk Ewelina Piktel Grzegorz Michalak Xiaobo Gu Alina Kułakowska Paul B. Savage Robert Bucki |
| author_sort | Katarzyna Niemirowicz |
| collection | DOAJ |
| description | Abstract Fungal infections caused by Candida spp. represent an emerging problem during treatment of immunocompromised patients and those hospitalized with serious principal diseases. The ever-growing number of fungal strains exhibiting drug resistance necessitates the development of novel antimicrobial therapies including those based on membrane-permeabilizing agents and nanomaterials as drug carriers. In this study, the fungicidal activities of LL-37 peptide, ceragenin CSA-13 and its magnetic derivatives (MNP@LL-37, MNP@CSA-13) against laboratory and clinical strains of C. albicans, C. glabrata and C. tropicalis were evaluated. These experiments confirm the high anti-fungal activity of these well-characterized agents mediated by their interaction with the fungal membrane and demonstrate elevated activity following immobilization of LL-37 and CSA-13 on the surface of magnetic nanoparticles (MNPs). Furthermore, MNP-based nanosystems are resistant to inhibitory factors present in body fluids and effectively inhibit formation of fungal biofilm. Simultaneously, synthesized nanostructures maintain immunomodulatory properties, described previously for free LL-37 peptide and CSA-13 substrate and they do not interfere with the proliferation and viability of osteoblasts, confirming their high biocompatibility. |
| first_indexed | 2024-12-20T20:10:08Z |
| format | Article |
| id | doaj.art-7413342e159a47cb9f47c8beb2b5f9fb |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-20T20:10:08Z |
| publishDate | 2017-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-7413342e159a47cb9f47c8beb2b5f9fb2022-12-21T19:27:51ZengNature PortfolioScientific Reports2045-23222017-07-017111210.1038/s41598-017-04653-1Formulation and candidacidal activity of magnetic nanoparticles coated with cathelicidin LL-37 and ceragenin CSA-13Katarzyna Niemirowicz0Bonita Durnaś1Grażyna Tokajuk2Ewelina Piktel3Grzegorz Michalak4Xiaobo Gu5Alina Kułakowska6Paul B. Savage7Robert Bucki8Department of Microbiological and Nanobiomedical Engineering, Medical University of BialystokDepartment of Microbiology and Immunology, The Faculty of Health Sciences of the Jan Kochanowski University in KielceDepartment of Periodontal and Oral Mucosa Diseases, Medical University of BialystokDepartment of Microbiological and Nanobiomedical Engineering, Medical University of BialystokDepartment of Microbiological and Nanobiomedical Engineering, Medical University of BialystokDepartment of Chemistry and Biochemistry, Brigham Young UniversityDepartment of Neurology, Medical University of BiałystokDepartment of Chemistry and Biochemistry, Brigham Young UniversityDepartment of Microbiological and Nanobiomedical Engineering, Medical University of BialystokAbstract Fungal infections caused by Candida spp. represent an emerging problem during treatment of immunocompromised patients and those hospitalized with serious principal diseases. The ever-growing number of fungal strains exhibiting drug resistance necessitates the development of novel antimicrobial therapies including those based on membrane-permeabilizing agents and nanomaterials as drug carriers. In this study, the fungicidal activities of LL-37 peptide, ceragenin CSA-13 and its magnetic derivatives (MNP@LL-37, MNP@CSA-13) against laboratory and clinical strains of C. albicans, C. glabrata and C. tropicalis were evaluated. These experiments confirm the high anti-fungal activity of these well-characterized agents mediated by their interaction with the fungal membrane and demonstrate elevated activity following immobilization of LL-37 and CSA-13 on the surface of magnetic nanoparticles (MNPs). Furthermore, MNP-based nanosystems are resistant to inhibitory factors present in body fluids and effectively inhibit formation of fungal biofilm. Simultaneously, synthesized nanostructures maintain immunomodulatory properties, described previously for free LL-37 peptide and CSA-13 substrate and they do not interfere with the proliferation and viability of osteoblasts, confirming their high biocompatibility.https://doi.org/10.1038/s41598-017-04653-1 |
| spellingShingle | Katarzyna Niemirowicz Bonita Durnaś Grażyna Tokajuk Ewelina Piktel Grzegorz Michalak Xiaobo Gu Alina Kułakowska Paul B. Savage Robert Bucki Formulation and candidacidal activity of magnetic nanoparticles coated with cathelicidin LL-37 and ceragenin CSA-13 Scientific Reports |
| title | Formulation and candidacidal activity of magnetic nanoparticles coated with cathelicidin LL-37 and ceragenin CSA-13 |
| title_full | Formulation and candidacidal activity of magnetic nanoparticles coated with cathelicidin LL-37 and ceragenin CSA-13 |
| title_fullStr | Formulation and candidacidal activity of magnetic nanoparticles coated with cathelicidin LL-37 and ceragenin CSA-13 |
| title_full_unstemmed | Formulation and candidacidal activity of magnetic nanoparticles coated with cathelicidin LL-37 and ceragenin CSA-13 |
| title_short | Formulation and candidacidal activity of magnetic nanoparticles coated with cathelicidin LL-37 and ceragenin CSA-13 |
| title_sort | formulation and candidacidal activity of magnetic nanoparticles coated with cathelicidin ll 37 and ceragenin csa 13 |
| url | https://doi.org/10.1038/s41598-017-04653-1 |
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