Effects in rats of bee-wax alcohols (D-002) on ulcerative colitis induced by dextran sulfate and ethanol
Objectives. To investigate the effects of D-002, a mixture of 6 high molecular weight primary aliphatic alcohols, obtained from beeswax (Apis mellifera), on severe inflammatory ulcerative colitis (UC) induced by Dextran sulfate (DSS) and ethanol in rats (Ratus ratus). Materials and methods. Rats wer...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | Spanish |
| Published: |
Instituto Nacional de Salud
2017-06-01
|
| Series: | Revista Peruana de Medicina Experimental y Salud Pública |
| Subjects: | |
| Online Access: | https://rpmesp.ins.gob.pe/index.php/rpmesp/article/view/2369 |
| _version_ | 1818149530496925696 |
|---|---|
| author | Vivian Molina-Cuevas Yazmin Ravelo-Calzado Zullyt Zamora-Rodríguez Miriam Noa-Puig Maikel Valle-Clara Yohani Pérez-Guerra Ámbar Oyarzabal-Yera Sonia Jiménez-Despaigne Rosa Mas-Ferreiro |
| author_facet | Vivian Molina-Cuevas Yazmin Ravelo-Calzado Zullyt Zamora-Rodríguez Miriam Noa-Puig Maikel Valle-Clara Yohani Pérez-Guerra Ámbar Oyarzabal-Yera Sonia Jiménez-Despaigne Rosa Mas-Ferreiro |
| author_sort | Vivian Molina-Cuevas |
| collection | DOAJ |
| description | Objectives. To investigate the effects of D-002, a mixture of 6 high molecular weight primary aliphatic alcohols, obtained from beeswax (Apis mellifera), on severe inflammatory ulcerative colitis (UC) induced by Dextran sulfate (DSS) and ethanol in rats (Ratus ratus). Materials and methods. Rats were randomly distributed in six groups: a zero control to which no damage was caused, and five to which the UC was induced: a negative control (vehicle), three treated with D-002 (25, 100 and 400 mg/kg) and a positive control with sulfasalazine (200 mg/kg) (reference substance). Clinical manifestations (body weight variation, diarrhea and rectal bleeding), macroscopic and histological damage score, and myeloperoxidase (MPO) activity were quantified. Results. The oral treatment with D-002 (25, 100 and 400 mg/kg) significantly prevented the decrease in body weight. The dose of 400 mg/kg reduced the presence of diarrhea and rectal bleeding, although its comparison with the negative control only reached statistical significance on diarrhea. D-002 (25, 100 and 400 mg/kg) significantly reduced the score of macroscopic lesions (40.0; 43.3 and 47.2% inhibition, respectively), the histological damage score (31.5; 53.7 and 67.1% inhibition, respectively) and the activity of MPO (73.2; 83.6 and 85.0% inhibition, respectively), compared to the negative control group. Sulfasalazine significantly reduced all variables studied. Conclusions. D-002 (25, 100 and 400 mg/kg) significantly protected the colonic mucosa in rats with severe inflammatory UC induced by DSS and ethanol. |
| first_indexed | 2024-12-11T13:08:30Z |
| format | Article |
| id | doaj.art-741349a902734e7d8e0c5662b5ce4aac |
| institution | Directory Open Access Journal |
| issn | 1726-4634 1726-4642 |
| language | Spanish |
| last_indexed | 2024-12-11T13:08:30Z |
| publishDate | 2017-06-01 |
| publisher | Instituto Nacional de Salud |
| record_format | Article |
| series | Revista Peruana de Medicina Experimental y Salud Pública |
| spelling | doaj.art-741349a902734e7d8e0c5662b5ce4aac2022-12-22T01:06:14ZspaInstituto Nacional de SaludRevista Peruana de Medicina Experimental y Salud Pública1726-46341726-46422017-06-013421768210.17843/rpmesp.2017.342.23691884Effects in rats of bee-wax alcohols (D-002) on ulcerative colitis induced by dextran sulfate and ethanolVivian Molina-Cuevas0Yazmin Ravelo-Calzado1Zullyt Zamora-Rodríguez2Miriam Noa-Puig3Maikel Valle-Clara4Yohani Pérez-Guerra5Ámbar Oyarzabal-Yera6Sonia Jiménez-Despaigne7Rosa Mas-Ferreiro8Centro de Productos Naturales, Centro Nacional de Investigaciones Científicas, La Habana, Cuba. Licenciada en BioquímicaCentro de Productos Naturales, Centro Nacional de Investigaciones Científicas, La Habana, Cuba. licenciada en Ciencias FarmacéuticasCentro de Productos Naturales, Centro Nacional de Investigaciones Científicas, La Habana, Cuba. doctora en Medicina VeterinariaCentro de Productos Naturales, Centro Nacional de Investigaciones Científicas, La Habana, Cuba. doctora en Ciencias MédicasCentro de Productos Naturales, Centro Nacional de Investigaciones Científicas, La Habana, Cuba. doctora en Medicina VeterinariaCentro de Productos Naturales, Centro Nacional de Investigaciones Científicas, La Habana, Cuba. licenciada en Ciencias Farmacéuticas,Centro de Productos Naturales, Centro Nacional de Investigaciones Científicas, La Habana, Cuba. licenciada en Ciencias Farmacéuticas,Centro de Productos Naturales, Centro Nacional de Investigaciones Científicas, La Habana, Cuba. tecnólogo en QuímicaCentro de Productos Naturales, Centro Nacional de Investigaciones Científicas, La Habana, Cuba. Licenciada en BioquímicaObjectives. To investigate the effects of D-002, a mixture of 6 high molecular weight primary aliphatic alcohols, obtained from beeswax (Apis mellifera), on severe inflammatory ulcerative colitis (UC) induced by Dextran sulfate (DSS) and ethanol in rats (Ratus ratus). Materials and methods. Rats were randomly distributed in six groups: a zero control to which no damage was caused, and five to which the UC was induced: a negative control (vehicle), three treated with D-002 (25, 100 and 400 mg/kg) and a positive control with sulfasalazine (200 mg/kg) (reference substance). Clinical manifestations (body weight variation, diarrhea and rectal bleeding), macroscopic and histological damage score, and myeloperoxidase (MPO) activity were quantified. Results. The oral treatment with D-002 (25, 100 and 400 mg/kg) significantly prevented the decrease in body weight. The dose of 400 mg/kg reduced the presence of diarrhea and rectal bleeding, although its comparison with the negative control only reached statistical significance on diarrhea. D-002 (25, 100 and 400 mg/kg) significantly reduced the score of macroscopic lesions (40.0; 43.3 and 47.2% inhibition, respectively), the histological damage score (31.5; 53.7 and 67.1% inhibition, respectively) and the activity of MPO (73.2; 83.6 and 85.0% inhibition, respectively), compared to the negative control group. Sulfasalazine significantly reduced all variables studied. Conclusions. D-002 (25, 100 and 400 mg/kg) significantly protected the colonic mucosa in rats with severe inflammatory UC induced by DSS and ethanol.https://rpmesp.ins.gob.pe/index.php/rpmesp/article/view/2369abejascolitis ulcerosaratas |
| spellingShingle | Vivian Molina-Cuevas Yazmin Ravelo-Calzado Zullyt Zamora-Rodríguez Miriam Noa-Puig Maikel Valle-Clara Yohani Pérez-Guerra Ámbar Oyarzabal-Yera Sonia Jiménez-Despaigne Rosa Mas-Ferreiro Effects in rats of bee-wax alcohols (D-002) on ulcerative colitis induced by dextran sulfate and ethanol Revista Peruana de Medicina Experimental y Salud Pública abejas colitis ulcerosa ratas |
| title | Effects in rats of bee-wax alcohols (D-002) on ulcerative colitis induced by dextran sulfate and ethanol |
| title_full | Effects in rats of bee-wax alcohols (D-002) on ulcerative colitis induced by dextran sulfate and ethanol |
| title_fullStr | Effects in rats of bee-wax alcohols (D-002) on ulcerative colitis induced by dextran sulfate and ethanol |
| title_full_unstemmed | Effects in rats of bee-wax alcohols (D-002) on ulcerative colitis induced by dextran sulfate and ethanol |
| title_short | Effects in rats of bee-wax alcohols (D-002) on ulcerative colitis induced by dextran sulfate and ethanol |
| title_sort | effects in rats of bee wax alcohols d 002 on ulcerative colitis induced by dextran sulfate and ethanol |
| topic | abejas colitis ulcerosa ratas |
| url | https://rpmesp.ins.gob.pe/index.php/rpmesp/article/view/2369 |
| work_keys_str_mv | AT vivianmolinacuevas effectsinratsofbeewaxalcoholsd002onulcerativecolitisinducedbydextransulfateandethanol AT yazminravelocalzado effectsinratsofbeewaxalcoholsd002onulcerativecolitisinducedbydextransulfateandethanol AT zullytzamorarodriguez effectsinratsofbeewaxalcoholsd002onulcerativecolitisinducedbydextransulfateandethanol AT miriamnoapuig effectsinratsofbeewaxalcoholsd002onulcerativecolitisinducedbydextransulfateandethanol AT maikelvalleclara effectsinratsofbeewaxalcoholsd002onulcerativecolitisinducedbydextransulfateandethanol AT yohaniperezguerra effectsinratsofbeewaxalcoholsd002onulcerativecolitisinducedbydextransulfateandethanol AT ambaroyarzabalyera effectsinratsofbeewaxalcoholsd002onulcerativecolitisinducedbydextransulfateandethanol AT soniajimenezdespaigne effectsinratsofbeewaxalcoholsd002onulcerativecolitisinducedbydextransulfateandethanol AT rosamasferreiro effectsinratsofbeewaxalcoholsd002onulcerativecolitisinducedbydextransulfateandethanol |