A Green Voltammetric Determination of Molnupiravir Using a Disposable Screen-Printed Reduced Graphene Oxide Electrode: Application for Pharmaceutical Dosage and Biological Fluid Forms
A new green-validated and highly sensitive electrochemical method for the determination of molnupiravir (MOV) has been developed using cyclic voltammetry. The proposed analytical platform involves the use of a disposable laboratory-made screen-printed reduced graphene oxide 2.5% modified electrode (...
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MDPI AG
2023-08-01
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| Online Access: | https://www.mdpi.com/2227-9040/11/9/471 |
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| author | Abdelrahman Nabil Hassan A. M. Hendawy Randa Abdel-Salam Rasha M. Ahmed Ahmed Shawky Samy Emara Noha Ibrahim |
| author_facet | Abdelrahman Nabil Hassan A. M. Hendawy Randa Abdel-Salam Rasha M. Ahmed Ahmed Shawky Samy Emara Noha Ibrahim |
| author_sort | Abdelrahman Nabil |
| collection | DOAJ |
| description | A new green-validated and highly sensitive electrochemical method for the determination of molnupiravir (MOV) has been developed using cyclic voltammetry. The proposed analytical platform involves the use of a disposable laboratory-made screen-printed reduced graphene oxide 2.5% modified electrode (rGO-SPCE 2.5%) for the first time to measure MOV with high specificity. The surface morphology of the sensor was investigated by using a scanning electron microscope armed with an energy-dispersive X-ray probe. The fabricated sensor attained improved sensitivity when sodium dodecyl sulfate (SDS) surfactant (3 µM) was added to the supporting electrolyte solution of 0.04 M Britton–Robinson buffer at pH 2. The electrochemical activity of rGO-SPCE was examined in comparison with two different working electrodes in order to demonstrate that it was the most competitive sensor for MOV monitoring. The method was validated using differential pulse voltammetry according to ICH guidelines, resulting in good precision, accuracy, specificity, and robustness over a concentration range of 0.152–18.272 µM, with a detection limit of 0.048 µM. The stability investigation demonstrated that rGO-SPCE 2.5% can provide high-stability behavior towards the analyte throughout a six-week period under refrigeration. The fabricated rGO-SPCE 2.5% was successfully employed for the measurement of MOV in pharmaceutical capsules and human biofluids without the interference of endogenous matrix components as well as the commonly used excipient. |
| first_indexed | 2024-03-10T22:55:19Z |
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| institution | Directory Open Access Journal |
| issn | 2227-9040 |
| language | English |
| last_indexed | 2024-03-10T22:55:19Z |
| publishDate | 2023-08-01 |
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| series | Chemosensors |
| spelling | doaj.art-7415f4c37c224dcca2e77a980f32f9cf2023-11-19T10:01:48ZengMDPI AGChemosensors2227-90402023-08-0111947110.3390/chemosensors11090471A Green Voltammetric Determination of Molnupiravir Using a Disposable Screen-Printed Reduced Graphene Oxide Electrode: Application for Pharmaceutical Dosage and Biological Fluid FormsAbdelrahman Nabil0Hassan A. M. Hendawy1Randa Abdel-Salam2Rasha M. Ahmed3Ahmed Shawky4Samy Emara5Noha Ibrahim6Pharmaceutical Chemistry Department, Faculty of Pharmacy, Misr International University, Km 28 Ismailia Road, Cairo 44971, EgyptChemistry Department, Egyptian Drug Authority, Giza 12512, EgyptAnalytical Chemistry Department, Faculty of Pharmacy, Suez Canal University Ismailia, Ismailia 41522, EgyptPharmaceutical Chemistry Department, Faculty of Pharmacy, Misr International University, Km 28 Ismailia Road, Cairo 44971, EgyptPharmaceutical Chemistry Department, Faculty of Pharmacy, Misr International University, Km 28 Ismailia Road, Cairo 44971, EgyptPharmaceutical Chemistry Department, Faculty of Pharmacy, Misr International University, Km 28 Ismailia Road, Cairo 44971, EgyptPharmaceutical Chemistry Department, Faculty of Pharmacy, Misr International University, Km 28 Ismailia Road, Cairo 44971, EgyptA new green-validated and highly sensitive electrochemical method for the determination of molnupiravir (MOV) has been developed using cyclic voltammetry. The proposed analytical platform involves the use of a disposable laboratory-made screen-printed reduced graphene oxide 2.5% modified electrode (rGO-SPCE 2.5%) for the first time to measure MOV with high specificity. The surface morphology of the sensor was investigated by using a scanning electron microscope armed with an energy-dispersive X-ray probe. The fabricated sensor attained improved sensitivity when sodium dodecyl sulfate (SDS) surfactant (3 µM) was added to the supporting electrolyte solution of 0.04 M Britton–Robinson buffer at pH 2. The electrochemical activity of rGO-SPCE was examined in comparison with two different working electrodes in order to demonstrate that it was the most competitive sensor for MOV monitoring. The method was validated using differential pulse voltammetry according to ICH guidelines, resulting in good precision, accuracy, specificity, and robustness over a concentration range of 0.152–18.272 µM, with a detection limit of 0.048 µM. The stability investigation demonstrated that rGO-SPCE 2.5% can provide high-stability behavior towards the analyte throughout a six-week period under refrigeration. The fabricated rGO-SPCE 2.5% was successfully employed for the measurement of MOV in pharmaceutical capsules and human biofluids without the interference of endogenous matrix components as well as the commonly used excipient.https://www.mdpi.com/2227-9040/11/9/471molnupiravircyclic voltammetrydifferential pulsedisposable screen-printed electrodereduced grapheme oxidebiological fluids |
| spellingShingle | Abdelrahman Nabil Hassan A. M. Hendawy Randa Abdel-Salam Rasha M. Ahmed Ahmed Shawky Samy Emara Noha Ibrahim A Green Voltammetric Determination of Molnupiravir Using a Disposable Screen-Printed Reduced Graphene Oxide Electrode: Application for Pharmaceutical Dosage and Biological Fluid Forms Chemosensors molnupiravir cyclic voltammetry differential pulse disposable screen-printed electrode reduced grapheme oxide biological fluids |
| title | A Green Voltammetric Determination of Molnupiravir Using a Disposable Screen-Printed Reduced Graphene Oxide Electrode: Application for Pharmaceutical Dosage and Biological Fluid Forms |
| title_full | A Green Voltammetric Determination of Molnupiravir Using a Disposable Screen-Printed Reduced Graphene Oxide Electrode: Application for Pharmaceutical Dosage and Biological Fluid Forms |
| title_fullStr | A Green Voltammetric Determination of Molnupiravir Using a Disposable Screen-Printed Reduced Graphene Oxide Electrode: Application for Pharmaceutical Dosage and Biological Fluid Forms |
| title_full_unstemmed | A Green Voltammetric Determination of Molnupiravir Using a Disposable Screen-Printed Reduced Graphene Oxide Electrode: Application for Pharmaceutical Dosage and Biological Fluid Forms |
| title_short | A Green Voltammetric Determination of Molnupiravir Using a Disposable Screen-Printed Reduced Graphene Oxide Electrode: Application for Pharmaceutical Dosage and Biological Fluid Forms |
| title_sort | green voltammetric determination of molnupiravir using a disposable screen printed reduced graphene oxide electrode application for pharmaceutical dosage and biological fluid forms |
| topic | molnupiravir cyclic voltammetry differential pulse disposable screen-printed electrode reduced grapheme oxide biological fluids |
| url | https://www.mdpi.com/2227-9040/11/9/471 |
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