Characterization of Chicken-Derived Genotype VII Newcastle Disease Virus Isolates from Northwest China
Newcastle disease virus (NDV) threatens global poultry production, with genotype VII the most prevalent strain in China. However, little information is available regarding viral multiplication and pathogenicity based inoculation route. The objectives of this study were to sequence NDV VII isolates a...
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| Format: | Article |
| Language: | English |
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Japan Poultry Science Association
2023-04-01
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| Series: | The Journal of Poultry Science |
| Subjects: | |
| Online Access: | https://www.jstage.jst.go.jp/article/jpsa/60/2/60_2023010/_article/-char/en |
| _version_ | 1797817434387775488 |
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| author | Xinxin Qiu Yanqing Jia Zhencang Zhang Xianglin Fo Wenhui Wang |
| author_facet | Xinxin Qiu Yanqing Jia Zhencang Zhang Xianglin Fo Wenhui Wang |
| author_sort | Xinxin Qiu |
| collection | DOAJ |
| description | Newcastle disease virus (NDV) threatens global poultry production, with genotype VII the most prevalent strain in China. However, little information is available regarding viral multiplication and pathogenicity based inoculation route. The objectives of this study were to sequence NDV VII isolates and to analyze their biological characteristics in detail. A total of 86 oral and cloacal swabs were collected from Shaanxi and Gansu provinces in northwest China. Identification of genotype VII NDV based on the M gene was performed by qPCR. Viral multiplication and pathogenicity were assessed as a function of route of infection. We observed increased morbidity and mortality using intravenous injection, whereas intranasal, intraocular, and cloacal infections resulted in slower progression and milder clinical disease, with viral proliferation obvious in different tissues. These results provide an important basis for the clinical control and prevention of NDV epidemics in poultry.
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| first_indexed | 2024-03-13T08:53:16Z |
| format | Article |
| id | doaj.art-74166cbef77749a8b40d29ece2b4afc6 |
| institution | Directory Open Access Journal |
| issn | 1349-0486 |
| language | English |
| last_indexed | 2024-03-13T08:53:16Z |
| publishDate | 2023-04-01 |
| publisher | Japan Poultry Science Association |
| record_format | Article |
| series | The Journal of Poultry Science |
| spelling | doaj.art-74166cbef77749a8b40d29ece2b4afc62023-05-29T06:18:46ZengJapan Poultry Science AssociationThe Journal of Poultry Science1349-04862023-04-01602https://doi.org/10.2141/jpsa.2023010Characterization of Chicken-Derived Genotype VII Newcastle Disease Virus Isolates from Northwest ChinaXinxin Qiu0Yanqing Jia1Zhencang Zhang2Xianglin Fo3Wenhui Wang4College of Veterinary Medicine, Gansu Agricultural University, Lanzhou, Gansu 730070, China. Department of animal engineering/Shaanxi engineering research center of the prevention and control for animal disease, Yangling Vocational & Technical College, Yangling, Shaanxi Province 712100, ChinaDepartment of animal engineering/Shaanxi engineering research center of the prevention and control for animal disease, Yangling Vocational & Technical College, Yangling, Shaanxi Province 712100, ChinaDepartment of animal engineering/Shaanxi engineering research center of the prevention and control for animal disease, Yangling Vocational & Technical College, Yangling, Shaanxi Province 712100, ChinaDepartment of animal engineering/Shaanxi engineering research center of the prevention and control for animal disease, Yangling Vocational & Technical College, Yangling, Shaanxi Province 712100, ChinaCollege of Veterinary Medicine, Gansu Agricultural University, Lanzhou, Gansu 730070, ChinaNewcastle disease virus (NDV) threatens global poultry production, with genotype VII the most prevalent strain in China. However, little information is available regarding viral multiplication and pathogenicity based inoculation route. The objectives of this study were to sequence NDV VII isolates and to analyze their biological characteristics in detail. A total of 86 oral and cloacal swabs were collected from Shaanxi and Gansu provinces in northwest China. Identification of genotype VII NDV based on the M gene was performed by qPCR. Viral multiplication and pathogenicity were assessed as a function of route of infection. We observed increased morbidity and mortality using intravenous injection, whereas intranasal, intraocular, and cloacal infections resulted in slower progression and milder clinical disease, with viral proliferation obvious in different tissues. These results provide an important basis for the clinical control and prevention of NDV epidemics in poultry. https://www.jstage.jst.go.jp/article/jpsa/60/2/60_2023010/_article/-char/eninfectioninoculation routendvphylogenetics |
| spellingShingle | Xinxin Qiu Yanqing Jia Zhencang Zhang Xianglin Fo Wenhui Wang Characterization of Chicken-Derived Genotype VII Newcastle Disease Virus Isolates from Northwest China The Journal of Poultry Science infection inoculation route ndv phylogenetics |
| title | Characterization of Chicken-Derived Genotype VII Newcastle Disease Virus Isolates from Northwest China |
| title_full | Characterization of Chicken-Derived Genotype VII Newcastle Disease Virus Isolates from Northwest China |
| title_fullStr | Characterization of Chicken-Derived Genotype VII Newcastle Disease Virus Isolates from Northwest China |
| title_full_unstemmed | Characterization of Chicken-Derived Genotype VII Newcastle Disease Virus Isolates from Northwest China |
| title_short | Characterization of Chicken-Derived Genotype VII Newcastle Disease Virus Isolates from Northwest China |
| title_sort | characterization of chicken derived genotype vii newcastle disease virus isolates from northwest china |
| topic | infection inoculation route ndv phylogenetics |
| url | https://www.jstage.jst.go.jp/article/jpsa/60/2/60_2023010/_article/-char/en |
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