Gene expression profiles in asbestos-exposed epithelial and mesothelial lung cell lines
<p>Abstract</p> <p>Background</p> <p>Asbestos has been shown to cause chromosomal damage and DNA aberrations. Exposure to asbestos causes many lung diseases e.g. asbestosis, malignant mesothelioma, and lung cancer, but the disease-related processes are still largely unk...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2007-03-01
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| Series: | BMC Genomics |
| Online Access: | http://www.biomedcentral.com/1471-2164/8/62 |
| _version_ | 1818857537445822464 |
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| author | Kaski Samuel Ruosaari Salla Lahti Leo Korpela Mikko V Lindholm Pamela M Nymark Penny Hollmén Jaakko Anttila Sisko Kinnula Vuokko L Knuutila Sakari |
| author_facet | Kaski Samuel Ruosaari Salla Lahti Leo Korpela Mikko V Lindholm Pamela M Nymark Penny Hollmén Jaakko Anttila Sisko Kinnula Vuokko L Knuutila Sakari |
| author_sort | Kaski Samuel |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Asbestos has been shown to cause chromosomal damage and DNA aberrations. Exposure to asbestos causes many lung diseases e.g. asbestosis, malignant mesothelioma, and lung cancer, but the disease-related processes are still largely unknown. We exposed the human cell lines A549, Beas-2B and Met5A to crocidolite asbestos and determined time-dependent gene expression profiles by using Affymetrix arrays. The hybridization data was analyzed by using an algorithm specifically designed for clustering of short time series expression data. A canonical correlation analysis was applied to identify correlations between the cell lines, and a Gene Ontology analysis method for the identification of enriched, differentially expressed biological processes.</p> <p>Results</p> <p>We recognized a large number of previously known as well as new potential asbestos-associated genes and biological processes, and identified chromosomal regions enriched with genes potentially contributing to common responses to asbestos in these cell lines. These include genes such as the thioredoxin domain containing gene (<it>TXNDC</it>) and the potential tumor suppressor, BCL2/adenovirus E1B 19kD-interacting protein gene (<it>BNIP3L</it>), GO-terms such as "positive regulation of I-kappaB kinase/NF-kappaB cascade" and "positive regulation of transcription, DNA-dependent", and chromosomal regions such as 2p22, 9p13, and 14q21. We present the complete data sets as Additional files.</p> <p>Conclusion</p> <p>This study identifies several interesting targets for further investigation in relation to asbestos-associated diseases.</p> |
| first_indexed | 2024-12-19T08:41:58Z |
| format | Article |
| id | doaj.art-74167783ca2a4de89aa1e2028c2e5fc6 |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-12-19T08:41:58Z |
| publishDate | 2007-03-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-74167783ca2a4de89aa1e2028c2e5fc62022-12-21T20:28:54ZengBMCBMC Genomics1471-21642007-03-01816210.1186/1471-2164-8-62Gene expression profiles in asbestos-exposed epithelial and mesothelial lung cell linesKaski SamuelRuosaari SallaLahti LeoKorpela Mikko VLindholm Pamela MNymark PennyHollmén JaakkoAnttila SiskoKinnula Vuokko LKnuutila Sakari<p>Abstract</p> <p>Background</p> <p>Asbestos has been shown to cause chromosomal damage and DNA aberrations. Exposure to asbestos causes many lung diseases e.g. asbestosis, malignant mesothelioma, and lung cancer, but the disease-related processes are still largely unknown. We exposed the human cell lines A549, Beas-2B and Met5A to crocidolite asbestos and determined time-dependent gene expression profiles by using Affymetrix arrays. The hybridization data was analyzed by using an algorithm specifically designed for clustering of short time series expression data. A canonical correlation analysis was applied to identify correlations between the cell lines, and a Gene Ontology analysis method for the identification of enriched, differentially expressed biological processes.</p> <p>Results</p> <p>We recognized a large number of previously known as well as new potential asbestos-associated genes and biological processes, and identified chromosomal regions enriched with genes potentially contributing to common responses to asbestos in these cell lines. These include genes such as the thioredoxin domain containing gene (<it>TXNDC</it>) and the potential tumor suppressor, BCL2/adenovirus E1B 19kD-interacting protein gene (<it>BNIP3L</it>), GO-terms such as "positive regulation of I-kappaB kinase/NF-kappaB cascade" and "positive regulation of transcription, DNA-dependent", and chromosomal regions such as 2p22, 9p13, and 14q21. We present the complete data sets as Additional files.</p> <p>Conclusion</p> <p>This study identifies several interesting targets for further investigation in relation to asbestos-associated diseases.</p>http://www.biomedcentral.com/1471-2164/8/62 |
| spellingShingle | Kaski Samuel Ruosaari Salla Lahti Leo Korpela Mikko V Lindholm Pamela M Nymark Penny Hollmén Jaakko Anttila Sisko Kinnula Vuokko L Knuutila Sakari Gene expression profiles in asbestos-exposed epithelial and mesothelial lung cell lines BMC Genomics |
| title | Gene expression profiles in asbestos-exposed epithelial and mesothelial lung cell lines |
| title_full | Gene expression profiles in asbestos-exposed epithelial and mesothelial lung cell lines |
| title_fullStr | Gene expression profiles in asbestos-exposed epithelial and mesothelial lung cell lines |
| title_full_unstemmed | Gene expression profiles in asbestos-exposed epithelial and mesothelial lung cell lines |
| title_short | Gene expression profiles in asbestos-exposed epithelial and mesothelial lung cell lines |
| title_sort | gene expression profiles in asbestos exposed epithelial and mesothelial lung cell lines |
| url | http://www.biomedcentral.com/1471-2164/8/62 |
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