Pectin Nanoparticle-Loaded Soft Coral <i>Nephthea</i> sp. Extract as <i>In Situ</i> Gel Enhances Chronic Wound Healing: <i>In Vitro</i>, <i>In Vivo</i>, and <i>In Silico</i> Studies
This study shed light for the first time on the <i>in vivo</i> diabetic wound healing potential activity of natural marine soft coral polymeric nanoparticle <i>in situ</i> gel using an excision wound model. A <i>Nephthea</i> sp. methanol–methylene chloride extract...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-07-01
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| Series: | Pharmaceuticals |
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| Online Access: | https://www.mdpi.com/1424-8247/16/7/957 |
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| author | Nevine H. Hassan Seham S. El-Hawary Mahmoud Emam Mohamed A. Rabeh Mohamed A. Tantawy Mohamed Seif Radwa M. A. Abd-Elal Gerhard Bringmann Usama Ramadan Abdelmohsen Nabil M. Selim |
| author_facet | Nevine H. Hassan Seham S. El-Hawary Mahmoud Emam Mohamed A. Rabeh Mohamed A. Tantawy Mohamed Seif Radwa M. A. Abd-Elal Gerhard Bringmann Usama Ramadan Abdelmohsen Nabil M. Selim |
| author_sort | Nevine H. Hassan |
| collection | DOAJ |
| description | This study shed light for the first time on the <i>in vivo</i> diabetic wound healing potential activity of natural marine soft coral polymeric nanoparticle <i>in situ</i> gel using an excision wound model. A <i>Nephthea</i> sp. methanol–methylene chloride extract loaded with pectin nanoparticles (LPNs) was created. For the preparation of <i>in situ</i> gel, ion-gelation techniques, the entrapment efficiency, the particle size, the polydispersity index, the zeta potential, the <i>in-vitro</i> drug release, and a transmission electron microscope were used and the best formula was selected. Using (UPLC-Q/TOF-MS), 27 secondary metabolites responsible for extract biological activity were identified. Isolation and identification of arachidic acid, oleic acid, nervonic acid, and bis-(2-ethylhexyl)-phthalate (DEHP) of <i>Nephthea</i> sp. was firstly reported here using NMR and mass spectral analyses. Moreover, LPN <i>in situ</i> gel has the best effects on regulating the proinflammatory cytokines (NF-κB, TNF-α, IL-6, and IL-1β) that were detected on days 7 and 15. The results were confirmed with an <i>in vitro</i> enzymatic inhibitory effect of the extract against glycogen synthase kinase (GSK-3) and matrix metalloproteinase-1 (MMP-1), with IC<sub>50</sub> values of 0.178 ± 0.009 and 0.258 ± 0.011 µg/mL, respectively. The molecular docking study showed a free binding energy of −9.6 kcal/mol for chabrolosteroid E, with the highest binding affinity for the enzyme (GSK-3), while isogosterone B had −7.8 kcal/mol for the enzyme (MMP-1). A pharmacokinetics study for chabrolohydroxybenzoquinone F and isogosterone B was performed, and it predicted the mode of action of wound healing activity. |
| first_indexed | 2024-03-11T00:44:53Z |
| format | Article |
| id | doaj.art-741b36e3d1e4432abc6e9d963011adc2 |
| institution | Directory Open Access Journal |
| issn | 1424-8247 |
| language | English |
| last_indexed | 2024-03-11T00:44:53Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceuticals |
| spelling | doaj.art-741b36e3d1e4432abc6e9d963011adc22023-11-18T20:52:21ZengMDPI AGPharmaceuticals1424-82472023-07-0116795710.3390/ph16070957Pectin Nanoparticle-Loaded Soft Coral <i>Nephthea</i> sp. Extract as <i>In Situ</i> Gel Enhances Chronic Wound Healing: <i>In Vitro</i>, <i>In Vivo</i>, and <i>In Silico</i> StudiesNevine H. Hassan0Seham S. El-Hawary1Mahmoud Emam2Mohamed A. Rabeh3Mohamed A. Tantawy4Mohamed Seif5Radwa M. A. Abd-Elal6Gerhard Bringmann7Usama Ramadan Abdelmohsen8Nabil M. Selim9Pharmacognosy Department, Faculty of Pharmacy, Modern University for Technology and Information, Cairo 11571, EgyptPharmacognosy Department, Faculty of Pharmacy, Cairo University, Giza 11562, EgyptPhytochemistry and Plant Systematics Department, National Research Centre, Dokki, Cairo 12622, EgyptPharmacognosy Department, College of Pharmacy, King Khalid University, Abha 62514, Saudi ArabiaHormones Department, Medical Research and Clinical Studies Institute, National Research Centre, Dokki, Cairo 12622, EgyptToxicology and Food Contaminants Department, Food Industries and Nutrition Research Institute, National Research Centre, Giza 12622, EgyptPharmaceutics and Drug Manufacturing Department, Faculty of Pharmacy, Modern University for Technology and Information, Cairo 11571, EgyptInstitute of Organic Chemistry, University of Würzburg, Am Hubland, 97074 Würzburg, GermanyPharmacognosy Department, Faculty of Pharmacy, Minia University, Minia 61519, EgyptPharmacognosy Department, Faculty of Pharmacy, Cairo University, Giza 11562, EgyptThis study shed light for the first time on the <i>in vivo</i> diabetic wound healing potential activity of natural marine soft coral polymeric nanoparticle <i>in situ</i> gel using an excision wound model. A <i>Nephthea</i> sp. methanol–methylene chloride extract loaded with pectin nanoparticles (LPNs) was created. For the preparation of <i>in situ</i> gel, ion-gelation techniques, the entrapment efficiency, the particle size, the polydispersity index, the zeta potential, the <i>in-vitro</i> drug release, and a transmission electron microscope were used and the best formula was selected. Using (UPLC-Q/TOF-MS), 27 secondary metabolites responsible for extract biological activity were identified. Isolation and identification of arachidic acid, oleic acid, nervonic acid, and bis-(2-ethylhexyl)-phthalate (DEHP) of <i>Nephthea</i> sp. was firstly reported here using NMR and mass spectral analyses. Moreover, LPN <i>in situ</i> gel has the best effects on regulating the proinflammatory cytokines (NF-κB, TNF-α, IL-6, and IL-1β) that were detected on days 7 and 15. The results were confirmed with an <i>in vitro</i> enzymatic inhibitory effect of the extract against glycogen synthase kinase (GSK-3) and matrix metalloproteinase-1 (MMP-1), with IC<sub>50</sub> values of 0.178 ± 0.009 and 0.258 ± 0.011 µg/mL, respectively. The molecular docking study showed a free binding energy of −9.6 kcal/mol for chabrolosteroid E, with the highest binding affinity for the enzyme (GSK-3), while isogosterone B had −7.8 kcal/mol for the enzyme (MMP-1). A pharmacokinetics study for chabrolohydroxybenzoquinone F and isogosterone B was performed, and it predicted the mode of action of wound healing activity.https://www.mdpi.com/1424-8247/16/7/957ADME<i>in situ</i> gelmolecular docking<i>Nephthea</i> sp.pectin nanoparticleswound healing |
| spellingShingle | Nevine H. Hassan Seham S. El-Hawary Mahmoud Emam Mohamed A. Rabeh Mohamed A. Tantawy Mohamed Seif Radwa M. A. Abd-Elal Gerhard Bringmann Usama Ramadan Abdelmohsen Nabil M. Selim Pectin Nanoparticle-Loaded Soft Coral <i>Nephthea</i> sp. Extract as <i>In Situ</i> Gel Enhances Chronic Wound Healing: <i>In Vitro</i>, <i>In Vivo</i>, and <i>In Silico</i> Studies Pharmaceuticals ADME <i>in situ</i> gel molecular docking <i>Nephthea</i> sp. pectin nanoparticles wound healing |
| title | Pectin Nanoparticle-Loaded Soft Coral <i>Nephthea</i> sp. Extract as <i>In Situ</i> Gel Enhances Chronic Wound Healing: <i>In Vitro</i>, <i>In Vivo</i>, and <i>In Silico</i> Studies |
| title_full | Pectin Nanoparticle-Loaded Soft Coral <i>Nephthea</i> sp. Extract as <i>In Situ</i> Gel Enhances Chronic Wound Healing: <i>In Vitro</i>, <i>In Vivo</i>, and <i>In Silico</i> Studies |
| title_fullStr | Pectin Nanoparticle-Loaded Soft Coral <i>Nephthea</i> sp. Extract as <i>In Situ</i> Gel Enhances Chronic Wound Healing: <i>In Vitro</i>, <i>In Vivo</i>, and <i>In Silico</i> Studies |
| title_full_unstemmed | Pectin Nanoparticle-Loaded Soft Coral <i>Nephthea</i> sp. Extract as <i>In Situ</i> Gel Enhances Chronic Wound Healing: <i>In Vitro</i>, <i>In Vivo</i>, and <i>In Silico</i> Studies |
| title_short | Pectin Nanoparticle-Loaded Soft Coral <i>Nephthea</i> sp. Extract as <i>In Situ</i> Gel Enhances Chronic Wound Healing: <i>In Vitro</i>, <i>In Vivo</i>, and <i>In Silico</i> Studies |
| title_sort | pectin nanoparticle loaded soft coral i nephthea i sp extract as i in situ i gel enhances chronic wound healing i in vitro i i in vivo i and i in silico i studies |
| topic | ADME <i>in situ</i> gel molecular docking <i>Nephthea</i> sp. pectin nanoparticles wound healing |
| url | https://www.mdpi.com/1424-8247/16/7/957 |
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