Antimicrobial, Antiproliferative and Antioxidant Activities of Eethanolic, Ethyl Acetate and Petroleum Ether Extracts of PsidiumGuajava Leaves
Background: To determine the antimicrobial, antiproliferative and antioxidant activities of ethanolic, ethyl acetate and petroleum ether extracts of Psidiumguajava leaves. Methods: The antimicrobial activity was assessed using disc diffusion method on Staphylococcus aureus, Bacillus cereus, Klebsiel...
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| Format: | Article |
| Language: | English |
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Rawalpindi Medical University
2016-09-01
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| Series: | Journal of Rawalpindi Medical College |
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| Online Access: | https://www.journalrmc.com/index.php/JRMC/article/view/183 |
| Summary: | Background: To determine the antimicrobial, antiproliferative
and antioxidant activities of ethanolic,
ethyl acetate and petroleum ether extracts of
Psidiumguajava leaves.
Methods: The antimicrobial activity was assessed
using disc diffusion method on Staphylococcus
aureus, Bacillus cereus, Klebsiella pneumoniae,
Pseudomonas aeruginosa, Escherichia coli and
Salmonella typhi. The anti-proliferative activity was
determined on HeLa cell lines. The anti-proliferative
activity was assessed using MTT assay while
antioxidant activity was determined using catalase
and superoxide dismutase (SOD) assays.
Results: The MTT results indicate that ethyl acetate
and petroleum ether extracts have significantly
inhibited the proliferation of HeLa cells as compared
to the control. Antioxidant assays showed that
catalase activity was insignificant for all extracts
however the SOD activity was significant. All the
extracts showed inhibitory zones against B. cereus
while only ethanolic extract of P. guajava leaves was
active against S. aureus.
Conclusion: Psidiumguajava plant leaves extracts
can be used as antiproliferative agent against HeLa
cell line |
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| ISSN: | 1683-3562 1683-3570 |