Cloning and expression analysis of ascorbate peroxidase gene BoAPX from Brassica oleracea var. italica(青花菜抗坏血酸过氧化物酶基因BoAPX的克隆与表达分析)

Cloning and expression analysis of ascorbate peroxidase gene BoAPX from Brassica oleracea var. italica(青花菜抗坏血酸过氧化物酶基因BoAPX的克隆与表达分析)

根据NCBI基因数据库提供的抗坏血酸过氧化物酶(Ascorbate Peroxidase，APX)基因序列设计简并引物，以青花菜（Brassica oleracea var. italica）叶片cDNA和DNA为材料进行PCR扩增，基因定名为BoAPX (Gen-Bank登录号：HQ871864）.BoAPX的基因组全长为1 394 bp，具7个内含子，编码区全长为753 bp，编码250个氨基酸残基.序列分析结果表明，BoAPX的N端中没有信号肽序列，为胞质型APX，BoAPX与已知APX具有较高的同源性.RT-PCR结果表明，BoAPX的表达受霜霉菌（Hyaloperonospora p...

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Bibliographic Details
Main Authors: JIANGMing(蒋明), MIAOLi-xiang(苗立祥), QIANBao-ying(钱宝英)
Format: Article
Language:zho
Published: Zhejiang University Press 2012-05-01
Series:Zhejiang Daxue xuebao. Lixue ban
Subjects:
青花菜
抗坏血酸过氧化物酶
克隆
表达分析
Online Access:https://doi.org/10.3785/j.issn.1008-9497.2012.03.022

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