Phenolics from grapefruit peels inhibit HMG-CoA reductase and angiotensin-I converting enzyme and show antioxidative properties in endothelial EA.Hy 926 cells
This study sought to investigate the possible mechanisms for the use of phenolic extracts from grapefruit peels in the management/prevention of cardiovascular complications. The effects of the phenolic extracts on key enzymes relevant to cardiovascular diseases [3-hydroxy-methyl-3-glutaryl coenzyme...
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| Format: | Article |
| Language: | English |
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Tsinghua University Press
2015-06-01
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| Series: | Food Science and Human Wellness |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453015000282 |
| _version_ | 1797706857212542976 |
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| author | Ayokunle O. Ademosun Ganiyu Oboh Sabina Passamonti Federica Tramer Lovro Ziberna Aline Augusti Boligon Margareth Linde Athayde |
| author_facet | Ayokunle O. Ademosun Ganiyu Oboh Sabina Passamonti Federica Tramer Lovro Ziberna Aline Augusti Boligon Margareth Linde Athayde |
| author_sort | Ayokunle O. Ademosun |
| collection | DOAJ |
| description | This study sought to investigate the possible mechanisms for the use of phenolic extracts from grapefruit peels in the management/prevention of cardiovascular complications. The effects of the phenolic extracts on key enzymes relevant to cardiovascular diseases [3-hydroxy-methyl-3-glutaryl coenzyme A reductase (HMG-CoA reductase) and angiotensin-I converting enzyme (ACE)], cellular antioxidant activity in human endothelial cells (EA.Hy 926) and radicals [1,1-diphenyl-2 picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS)] scavenging abilities were investigated. The phenolic contents of the extracts were investigated using HPLC–DAD. There was no significant (P > 0.05) difference in the HMG-CoA reductase inhibitory ability of the two extracts, while the bound phenolic extracts had a stronger ACE inhibitory ability than the soluble free phenolics. The extracts also showed intracellular antioxidant activity in human endothelial (EA.Hy 926) cells. Furthermore, the bound phenolics had significantly higher radicals (DPPH* and ABTS*) scavenging abilities than the free phenolics. The HPLC analysis revealed the presence of flavonoids (quercetin and kaempferol), phenolics acids (resveratrol, gallic acid, ellagic acid and caffeic acid) and tannin (catechin). The cellular antioxidative properties and inhibition of enzymes relevant to the management of cardiovascular complications showed that grapefruit peels could be used as nutraceuticals for the management of such conditions. |
| first_indexed | 2024-03-12T05:58:36Z |
| format | Article |
| id | doaj.art-741ec7d4e6fa4a0ea19c4c6618da11ed |
| institution | Directory Open Access Journal |
| issn | 2213-4530 |
| language | English |
| last_indexed | 2024-03-12T05:58:36Z |
| publishDate | 2015-06-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj.art-741ec7d4e6fa4a0ea19c4c6618da11ed2023-09-03T04:24:18ZengTsinghua University PressFood Science and Human Wellness2213-45302015-06-0142808510.1016/j.fshw.2015.05.002Phenolics from grapefruit peels inhibit HMG-CoA reductase and angiotensin-I converting enzyme and show antioxidative properties in endothelial EA.Hy 926 cellsAyokunle O. Ademosun0Ganiyu Oboh1Sabina Passamonti2Federica Tramer3Lovro Ziberna4Aline Augusti Boligon5Margareth Linde Athayde6Functional Foods and Nutraceuticals Unit, Department of Biochemistry, Federal University of Technology, Akure, NigeriaFunctional Foods and Nutraceuticals Unit, Department of Biochemistry, Federal University of Technology, Akure, NigeriaDepartment of Life Sciences, University of Trieste, via L. Giorgieri 1, 34127 Trieste, ItalyDepartment of Life Sciences, University of Trieste, via L. Giorgieri 1, 34127 Trieste, ItalyDepartment of Life Sciences, University of Trieste, via L. Giorgieri 1, 34127 Trieste, ItalyProgram of Post-Graduation in Pharmaceutical Sciences, Federal University of Santa Maria, Campus Camobi, Santa Maria, RS 97105-900, BrazilProgram of Post-Graduation in Pharmaceutical Sciences, Federal University of Santa Maria, Campus Camobi, Santa Maria, RS 97105-900, BrazilThis study sought to investigate the possible mechanisms for the use of phenolic extracts from grapefruit peels in the management/prevention of cardiovascular complications. The effects of the phenolic extracts on key enzymes relevant to cardiovascular diseases [3-hydroxy-methyl-3-glutaryl coenzyme A reductase (HMG-CoA reductase) and angiotensin-I converting enzyme (ACE)], cellular antioxidant activity in human endothelial cells (EA.Hy 926) and radicals [1,1-diphenyl-2 picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS)] scavenging abilities were investigated. The phenolic contents of the extracts were investigated using HPLC–DAD. There was no significant (P > 0.05) difference in the HMG-CoA reductase inhibitory ability of the two extracts, while the bound phenolic extracts had a stronger ACE inhibitory ability than the soluble free phenolics. The extracts also showed intracellular antioxidant activity in human endothelial (EA.Hy 926) cells. Furthermore, the bound phenolics had significantly higher radicals (DPPH* and ABTS*) scavenging abilities than the free phenolics. The HPLC analysis revealed the presence of flavonoids (quercetin and kaempferol), phenolics acids (resveratrol, gallic acid, ellagic acid and caffeic acid) and tannin (catechin). The cellular antioxidative properties and inhibition of enzymes relevant to the management of cardiovascular complications showed that grapefruit peels could be used as nutraceuticals for the management of such conditions.http://www.sciencedirect.com/science/article/pii/S2213453015000282Endothelial cellsAntioxidantAngiotensin-I converting enzymeGrapefruit peelsHMG-CoA reductase |
| spellingShingle | Ayokunle O. Ademosun Ganiyu Oboh Sabina Passamonti Federica Tramer Lovro Ziberna Aline Augusti Boligon Margareth Linde Athayde Phenolics from grapefruit peels inhibit HMG-CoA reductase and angiotensin-I converting enzyme and show antioxidative properties in endothelial EA.Hy 926 cells Food Science and Human Wellness Endothelial cells Antioxidant Angiotensin-I converting enzyme Grapefruit peels HMG-CoA reductase |
| title | Phenolics from grapefruit peels inhibit HMG-CoA reductase and angiotensin-I converting enzyme and show antioxidative properties in endothelial EA.Hy 926 cells |
| title_full | Phenolics from grapefruit peels inhibit HMG-CoA reductase and angiotensin-I converting enzyme and show antioxidative properties in endothelial EA.Hy 926 cells |
| title_fullStr | Phenolics from grapefruit peels inhibit HMG-CoA reductase and angiotensin-I converting enzyme and show antioxidative properties in endothelial EA.Hy 926 cells |
| title_full_unstemmed | Phenolics from grapefruit peels inhibit HMG-CoA reductase and angiotensin-I converting enzyme and show antioxidative properties in endothelial EA.Hy 926 cells |
| title_short | Phenolics from grapefruit peels inhibit HMG-CoA reductase and angiotensin-I converting enzyme and show antioxidative properties in endothelial EA.Hy 926 cells |
| title_sort | phenolics from grapefruit peels inhibit hmg coa reductase and angiotensin i converting enzyme and show antioxidative properties in endothelial ea hy 926 cells |
| topic | Endothelial cells Antioxidant Angiotensin-I converting enzyme Grapefruit peels HMG-CoA reductase |
| url | http://www.sciencedirect.com/science/article/pii/S2213453015000282 |
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