Functional markers developed from TaGS3, a negative regulator of grain weight and size, for marker-assisted selection in wheat
The TaGS3 homoeologous genes (homoeologs) located on chromosomes 7A, 4A, and 7D in hexaploid wheat were cloned. Relative expression analysis of the three TaGS3 homoeologs revealed that the expression levels of TaGS3-4A and TaGS3-7D in developing grains were higher than that of TaGS3-7A. Genetic evid...
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KeAi Communications Co., Ltd.
2020-12-01
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| Series: | Crop Journal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214514120300490 |
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| author | Wei Zhang Huifang Li Liya Zhi Qiannan Su Jiajia Liu Xiaoli Ren Deyuan Meng Na Zhang Jun Ji Xueyong Zhang Junming Li |
| author_facet | Wei Zhang Huifang Li Liya Zhi Qiannan Su Jiajia Liu Xiaoli Ren Deyuan Meng Na Zhang Jun Ji Xueyong Zhang Junming Li |
| author_sort | Wei Zhang |
| collection | DOAJ |
| description | The TaGS3 homoeologous genes (homoeologs) located on chromosomes 7A, 4A, and 7D in hexaploid wheat were cloned. Relative expression analysis of the three TaGS3 homoeologs revealed that the expression levels of TaGS3-4A and TaGS3-7D in developing grains were higher than that of TaGS3-7A. Genetic evidence showed that TaGS3 was a negative regulator of grain weight and grain size. Fifteen polymorphic sites and five haplotypes were detected in TaGS3-4A. Two molecular markers were developed to distinguish the five haplotypes. Association analysis using 260 accessions from Chinese wheat mini-core collection (MCC) indicated that TaGS3-4A affected thousand grain weight (TGW) and grain length (GL). HAP-4A-1 and HAP-4A-2 were favorable haplotypes that increased TGW and GL and had undergone strong selection during domestication of wheat. In addition, interaction of the TaGS3-4A and TaGS3-7D homoeologs had significant additive effects on the grain traits. Hap-4A-1/Hap-7D-2 was the best haplotype combination in increasing TGW and GL. The frequencies and geographic distributions of favorable TaGS3 haplotypes among 1388 wheat accessions from worldwide sources provided clues for selection of yield-related traits. Our findings demonstrated that TaGS3-4A had significant effects on TGW and GL. Marker-assisted selection of HAP-4A-1/2 combined with HAP-7D-2 has potential to increase wheat yields. |
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| last_indexed | 2024-12-13T10:37:13Z |
| publishDate | 2020-12-01 |
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| series | Crop Journal |
| spelling | doaj.art-741fcb0742be4105a22bd88d739fb0fa2022-12-21T23:50:41ZengKeAi Communications Co., Ltd.Crop Journal2214-51412020-12-0186943952Functional markers developed from TaGS3, a negative regulator of grain weight and size, for marker-assisted selection in wheatWei Zhang0Huifang Li1Liya Zhi2Qiannan Su3Jiajia Liu4Xiaoli Ren5Deyuan Meng6Na Zhang7Jun Ji8Xueyong Zhang9Junming Li10Hebei University of Economics and Business, Shijiazhuang 050061, Hebei, ChinaKey Laboratory of Crop Gene Resources and Germplasm Enhancement, Ministry of Agriculture/The National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaCenter for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang 050022, Hebei, ChinaCenter for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang 050022, Hebei, ChinaCenter for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang 050022, Hebei, ChinaCenter for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang 050022, Hebei, ChinaCenter for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang 050022, Hebei, ChinaCenter for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang 050022, Hebei, China; State Key Laboratory of Plant Cell and Chromosome Engineering, Chinese Academy of Sciences, Beijing 100101, China; The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing 100101, ChinaCenter for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang 050022, Hebei, China; State Key Laboratory of Plant Cell and Chromosome Engineering, Chinese Academy of Sciences, Beijing 100101, China; The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing 100101, ChinaKey Laboratory of Crop Gene Resources and Germplasm Enhancement, Ministry of Agriculture/The National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China; Corresponding authors.Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang 050022, Hebei, China; State Key Laboratory of Plant Cell and Chromosome Engineering, Chinese Academy of Sciences, Beijing 100101, China; The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing 100101, China; Corresponding authors.The TaGS3 homoeologous genes (homoeologs) located on chromosomes 7A, 4A, and 7D in hexaploid wheat were cloned. Relative expression analysis of the three TaGS3 homoeologs revealed that the expression levels of TaGS3-4A and TaGS3-7D in developing grains were higher than that of TaGS3-7A. Genetic evidence showed that TaGS3 was a negative regulator of grain weight and grain size. Fifteen polymorphic sites and five haplotypes were detected in TaGS3-4A. Two molecular markers were developed to distinguish the five haplotypes. Association analysis using 260 accessions from Chinese wheat mini-core collection (MCC) indicated that TaGS3-4A affected thousand grain weight (TGW) and grain length (GL). HAP-4A-1 and HAP-4A-2 were favorable haplotypes that increased TGW and GL and had undergone strong selection during domestication of wheat. In addition, interaction of the TaGS3-4A and TaGS3-7D homoeologs had significant additive effects on the grain traits. Hap-4A-1/Hap-7D-2 was the best haplotype combination in increasing TGW and GL. The frequencies and geographic distributions of favorable TaGS3 haplotypes among 1388 wheat accessions from worldwide sources provided clues for selection of yield-related traits. Our findings demonstrated that TaGS3-4A had significant effects on TGW and GL. Marker-assisted selection of HAP-4A-1/2 combined with HAP-7D-2 has potential to increase wheat yields.http://www.sciencedirect.com/science/article/pii/S2214514120300490TaGS3Grain yieldHaplotypeMASTriticum aestivum |
| spellingShingle | Wei Zhang Huifang Li Liya Zhi Qiannan Su Jiajia Liu Xiaoli Ren Deyuan Meng Na Zhang Jun Ji Xueyong Zhang Junming Li Functional markers developed from TaGS3, a negative regulator of grain weight and size, for marker-assisted selection in wheat Crop Journal TaGS3 Grain yield Haplotype MAS Triticum aestivum |
| title | Functional markers developed from TaGS3, a negative regulator of grain weight and size, for marker-assisted selection in wheat |
| title_full | Functional markers developed from TaGS3, a negative regulator of grain weight and size, for marker-assisted selection in wheat |
| title_fullStr | Functional markers developed from TaGS3, a negative regulator of grain weight and size, for marker-assisted selection in wheat |
| title_full_unstemmed | Functional markers developed from TaGS3, a negative regulator of grain weight and size, for marker-assisted selection in wheat |
| title_short | Functional markers developed from TaGS3, a negative regulator of grain weight and size, for marker-assisted selection in wheat |
| title_sort | functional markers developed from tags3 a negative regulator of grain weight and size for marker assisted selection in wheat |
| topic | TaGS3 Grain yield Haplotype MAS Triticum aestivum |
| url | http://www.sciencedirect.com/science/article/pii/S2214514120300490 |
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