Cryoprotection of porcine cornea: a scanning electron microscopy study
Porcine corneas were frozen with Me2SO, glycerol, 1,2-propanediol and PEG-400. The effects of the range of concentrations (5% and 10%) and temperature regimen (1ºC/min and 5ºC/min) were investigated. The integrity of corneal endothelial cells was evaluated by scanning electron microscopy and trypan...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Czech Academy of Agricultural Sciences
2005-05-01
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| Series: | Veterinární Medicína |
| Subjects: | |
| Online Access: | https://vetmed.agriculturejournals.cz/artkey/vet-200505-0006_cryoprotection-of-porcine-cornea-a-scanning-electron-microscopy-study.php |
| _version_ | 1797897730004090880 |
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| author | A. Neronov P. Giurov M. Cholakova M. Dimitrova E. Nikolova |
| author_facet | A. Neronov P. Giurov M. Cholakova M. Dimitrova E. Nikolova |
| author_sort | A. Neronov |
| collection | DOAJ |
| description | Porcine corneas were frozen with Me2SO, glycerol, 1,2-propanediol and PEG-400. The effects of the range of concentrations (5% and 10%) and temperature regimen (1ºC/min and 5ºC/min) were investigated. The integrity of corneal endothelial cells was evaluated by scanning electron microscopy and trypan blue staining. The presence of 5-10% PEG-400 in the protective medium was the most effective in minimizing changes in the integrity of the corneal endothelium during freezing-thawing procedures. |
| first_indexed | 2024-04-10T08:03:01Z |
| format | Article |
| id | doaj.art-7422fb7183c94ea0a095389d65355960 |
| institution | Directory Open Access Journal |
| issn | 0375-8427 1805-9392 |
| language | English |
| last_indexed | 2024-04-10T08:03:01Z |
| publishDate | 2005-05-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Veterinární Medicína |
| spelling | doaj.art-7422fb7183c94ea0a095389d653559602023-02-23T03:49:16ZengCzech Academy of Agricultural SciencesVeterinární Medicína0375-84271805-93922005-05-0150521922410.17221/5618-VETMEDvet-200505-0006Cryoprotection of porcine cornea: a scanning electron microscopy studyA. Neronov0P. Giurov1M. Cholakova2M. Dimitrova3E. Nikolova4Institute of Experimental Morphology and Anthropology, Bulgarian Academy of Sciences, Sofia, BulgariaInstitute of Experimental Pathology and Parasitology, Bulgarian Academy of Sciences, Sofia, BulgariaInstitute of Experimental Morphology and Anthropology, Bulgarian Academy of Sciences, Sofia, BulgariaInstitute of Experimental Morphology and Anthropology, Bulgarian Academy of Sciences, Sofia, BulgariaInstitute of Experimental Morphology and Anthropology, Bulgarian Academy of Sciences, Sofia, BulgariaPorcine corneas were frozen with Me2SO, glycerol, 1,2-propanediol and PEG-400. The effects of the range of concentrations (5% and 10%) and temperature regimen (1ºC/min and 5ºC/min) were investigated. The integrity of corneal endothelial cells was evaluated by scanning electron microscopy and trypan blue staining. The presence of 5-10% PEG-400 in the protective medium was the most effective in minimizing changes in the integrity of the corneal endothelium during freezing-thawing procedures.https://vetmed.agriculturejournals.cz/artkey/vet-200505-0006_cryoprotection-of-porcine-cornea-a-scanning-electron-microscopy-study.phpporcine corneame2soglycerol1,2-propanediolpeg-400 |
| spellingShingle | A. Neronov P. Giurov M. Cholakova M. Dimitrova E. Nikolova Cryoprotection of porcine cornea: a scanning electron microscopy study Veterinární Medicína porcine cornea me2so glycerol 1,2-propanediol peg-400 |
| title | Cryoprotection of porcine cornea: a scanning electron microscopy study |
| title_full | Cryoprotection of porcine cornea: a scanning electron microscopy study |
| title_fullStr | Cryoprotection of porcine cornea: a scanning electron microscopy study |
| title_full_unstemmed | Cryoprotection of porcine cornea: a scanning electron microscopy study |
| title_short | Cryoprotection of porcine cornea: a scanning electron microscopy study |
| title_sort | cryoprotection of porcine cornea a scanning electron microscopy study |
| topic | porcine cornea me2so glycerol 1,2-propanediol peg-400 |
| url | https://vetmed.agriculturejournals.cz/artkey/vet-200505-0006_cryoprotection-of-porcine-cornea-a-scanning-electron-microscopy-study.php |
| work_keys_str_mv | AT aneronov cryoprotectionofporcinecorneaascanningelectronmicroscopystudy AT pgiurov cryoprotectionofporcinecorneaascanningelectronmicroscopystudy AT mcholakova cryoprotectionofporcinecorneaascanningelectronmicroscopystudy AT mdimitrova cryoprotectionofporcinecorneaascanningelectronmicroscopystudy AT enikolova cryoprotectionofporcinecorneaascanningelectronmicroscopystudy |