Multilocus Sequence Typing of Non-JP2 Serotype b Aggregatibacter actinomycetemcomitans Strains of Ghanaian and Swedish Origin
Objective and MethodsThe Gram-negative bacterium, Aggregatibacter actinomycetemcomitans is associated with periodontitis affecting young individuals. The geographic dissemination of the highly leukotoxic JP2 genotype of serotype b of this species was previously studied by multilocus sequence typing...
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Frontiers Media S.A.
2021-12-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2021.769671/full |
| _version_ | 1819240749688946688 |
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| author | Rolf Claesson Anders Johansson Carola Höglund Åberg Anders Esberg Dorte Haubek Jan Oscarsson |
| author_facet | Rolf Claesson Anders Johansson Carola Höglund Åberg Anders Esberg Dorte Haubek Jan Oscarsson |
| author_sort | Rolf Claesson |
| collection | DOAJ |
| description | Objective and MethodsThe Gram-negative bacterium, Aggregatibacter actinomycetemcomitans is associated with periodontitis affecting young individuals. The geographic dissemination of the highly leukotoxic JP2 genotype of serotype b of this species was previously studied by multilocus sequence typing (MLST). Here, we have used MLST to genetically characterize non-JP2 genotype strains of serotype b, isolated from individuals living in Ghana (n=41), and in Sweden (n=13), respectively.ResultsThe MLST analysis revealed a total of nine sequence types (ST). Both Ghanaian and Swedish isolates were distributed in ST 1-3. ST 5 and 6 were only identified among the Ghanaian strains, whereas ST 4, 7, 8 and 9 were uniquely represented among the Swedish strains. Previously, we characterized these non-JP2 genotype strains of A. actinomycetemcomitans serotype b by arbitrarily-primed (AP)-PCR, which distributed them into three groups, AP-PCR type 1, 2, and 3, respectively. AP-PCR type 1 strains are generally highly leukotoxic, and are associated with progression of periodontal attachment loss. As AP-PCR type 1 includes both JP2 genotype strains and a proportion of non-JP2 genotype strains of serotype b, a straightforward diagnostic procedure has been sought. This has revealed a gene, cagE, which appears to be conserved only in this AP-PCR type. According to our results, MLST was not a highly discriminatory method to identify AP-PCR type 1, as strains of this AP-PCR type could be found within three different ST: ST 2, ST 3 and ST 8.ConclusionAccording to MLST, a geographic dissemination of non-JP2 genotype A. actinomycetemcomitans serotype b appears to exist. However, aiming to identify carriers of AP-PCR type 1, non-JP2 genotype serotype b, PCR with cagE-specific primers is likely the most efficient diagnostic procedure known today. |
| first_indexed | 2024-12-23T14:12:58Z |
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| id | doaj.art-7423055360904f9cb69875ba80221598 |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-12-23T14:12:58Z |
| publishDate | 2021-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-7423055360904f9cb69875ba802215982022-12-21T17:43:59ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882021-12-011110.3389/fcimb.2021.769671769671Multilocus Sequence Typing of Non-JP2 Serotype b Aggregatibacter actinomycetemcomitans Strains of Ghanaian and Swedish OriginRolf Claesson0Anders Johansson1Carola Höglund Åberg2Anders Esberg3Dorte Haubek4Jan Oscarsson5Division of Oral Microbiology, Department of Odontology, Umeå University, Umeå, SwedenDivision of Molecular Periodontology, Department of Odontology, Umeå University, Umeå, SwedenDivision of Molecular Periodontology, Department of Odontology, Umeå University, Umeå, SwedenDepartment of Odontology, Umeå University, Umeå, SwedenSection for Paediatric Dentistry, Department of Dentistry and Oral Health, Aarhus University, Aarhus, DenmarkDivision of Oral Microbiology, Department of Odontology, Umeå University, Umeå, SwedenObjective and MethodsThe Gram-negative bacterium, Aggregatibacter actinomycetemcomitans is associated with periodontitis affecting young individuals. The geographic dissemination of the highly leukotoxic JP2 genotype of serotype b of this species was previously studied by multilocus sequence typing (MLST). Here, we have used MLST to genetically characterize non-JP2 genotype strains of serotype b, isolated from individuals living in Ghana (n=41), and in Sweden (n=13), respectively.ResultsThe MLST analysis revealed a total of nine sequence types (ST). Both Ghanaian and Swedish isolates were distributed in ST 1-3. ST 5 and 6 were only identified among the Ghanaian strains, whereas ST 4, 7, 8 and 9 were uniquely represented among the Swedish strains. Previously, we characterized these non-JP2 genotype strains of A. actinomycetemcomitans serotype b by arbitrarily-primed (AP)-PCR, which distributed them into three groups, AP-PCR type 1, 2, and 3, respectively. AP-PCR type 1 strains are generally highly leukotoxic, and are associated with progression of periodontal attachment loss. As AP-PCR type 1 includes both JP2 genotype strains and a proportion of non-JP2 genotype strains of serotype b, a straightforward diagnostic procedure has been sought. This has revealed a gene, cagE, which appears to be conserved only in this AP-PCR type. According to our results, MLST was not a highly discriminatory method to identify AP-PCR type 1, as strains of this AP-PCR type could be found within three different ST: ST 2, ST 3 and ST 8.ConclusionAccording to MLST, a geographic dissemination of non-JP2 genotype A. actinomycetemcomitans serotype b appears to exist. However, aiming to identify carriers of AP-PCR type 1, non-JP2 genotype serotype b, PCR with cagE-specific primers is likely the most efficient diagnostic procedure known today.https://www.frontiersin.org/articles/10.3389/fcimb.2021.769671/fullAggregatibacter actinomycetemcomitansmultilocus sequence typing (MLST)non-JP2serotype bcagEevolutionary analysis |
| spellingShingle | Rolf Claesson Anders Johansson Carola Höglund Åberg Anders Esberg Dorte Haubek Jan Oscarsson Multilocus Sequence Typing of Non-JP2 Serotype b Aggregatibacter actinomycetemcomitans Strains of Ghanaian and Swedish Origin Frontiers in Cellular and Infection Microbiology Aggregatibacter actinomycetemcomitans multilocus sequence typing (MLST) non-JP2 serotype b cagE evolutionary analysis |
| title | Multilocus Sequence Typing of Non-JP2 Serotype b Aggregatibacter actinomycetemcomitans Strains of Ghanaian and Swedish Origin |
| title_full | Multilocus Sequence Typing of Non-JP2 Serotype b Aggregatibacter actinomycetemcomitans Strains of Ghanaian and Swedish Origin |
| title_fullStr | Multilocus Sequence Typing of Non-JP2 Serotype b Aggregatibacter actinomycetemcomitans Strains of Ghanaian and Swedish Origin |
| title_full_unstemmed | Multilocus Sequence Typing of Non-JP2 Serotype b Aggregatibacter actinomycetemcomitans Strains of Ghanaian and Swedish Origin |
| title_short | Multilocus Sequence Typing of Non-JP2 Serotype b Aggregatibacter actinomycetemcomitans Strains of Ghanaian and Swedish Origin |
| title_sort | multilocus sequence typing of non jp2 serotype b aggregatibacter actinomycetemcomitans strains of ghanaian and swedish origin |
| topic | Aggregatibacter actinomycetemcomitans multilocus sequence typing (MLST) non-JP2 serotype b cagE evolutionary analysis |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2021.769671/full |
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