Association of Follicular Fluid Antioxidants Activity with Aging and In Vitro Fertilization Outcome: A Cross-Sectional Study
Background: This research was aimed at assessing the relationship between the follicular fluid (FF) antioxidantsactivity, aging and in vitro fertilization (IVF) outcome.Materials and Methods: The present cross-sectional study was carried out on 65 women undergoing IVF/intracytoplasmicsperm injection...
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| Format: | Article |
| Language: | English |
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Royan Institute (ACECR), Tehran
2024-04-01
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| Series: | International Journal of Fertility and Sterility |
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| Online Access: | https://www.ijfs.ir/article_705588_caac522492efa99c44c7d35ad248f7bd.pdf |
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| author | Mahsa Afrough Roshan Nikbakht Mahmoud Hashemitabar Elham Ghalambaz Sareh Amirzadeh Adel Zardkaf Sahar Adham Maryam Mehdipour Parvin Dorfeshan |
| author_facet | Mahsa Afrough Roshan Nikbakht Mahmoud Hashemitabar Elham Ghalambaz Sareh Amirzadeh Adel Zardkaf Sahar Adham Maryam Mehdipour Parvin Dorfeshan |
| author_sort | Mahsa Afrough |
| collection | DOAJ |
| description | Background: This research was aimed at assessing the relationship between the follicular fluid (FF) antioxidantsactivity, aging and in vitro fertilization (IVF) outcome.Materials and Methods: The present cross-sectional study was carried out on 65 women undergoing IVF/intracytoplasmicsperm injection (IVF/ICSI) cycles due to unexplained infertility. Ovarian stimulation was performed using thelong gonadotropin-releasing hormone (GnRH) agonist protocol. After ovum pickup, FF was collected and processedto measure the level of superoxide dismutase (SOD), catalase (CAT) activity, total antioxidant capacity (TAC) andglutathione (GSH). Day 3 after ICSI, fresh embryos were transferred and later, possible pregnancy was assessed.Patients participating in this study were divided into four groups on the basis of age and pregnancy outcome.Results: SOD activity was not significantly different between the groups (P=0.218). GSH in the group whose participantswere aged ≤35 years and were pregnant was higher than that in other groups. CAT activity in groups withyounger participants was higher compared to the other groups. The mean TAC was higher in groups with pregnantparticipants compared to the non-pregnant women. Correlation analysis showed that: GSH level had a significantnegative correlation with age (P<0.001, R -0.55) and a significant positive correlation with pregnancy (P=0.015,R=0.30). CAT level also had a significant negative correlation with age (P<0.001, R=-0.42) and the level of TAC hada significant positive correlation with pregnancy (P<0.001, R=0.59).Conclusion: According to our results, the levels of TAC, GSH and CAT in younger and pregnant women were highercompared with those undergoing ICSI cycles. Given the correlation of FF antioxidant activity with age and pregnancy,it is necessary to carry out more research on these compounds and the maintenance of pregnancy. |
| first_indexed | 2024-03-07T23:54:57Z |
| format | Article |
| id | doaj.art-7423ff16463a429a8338f1529c33f472 |
| institution | Directory Open Access Journal |
| issn | 2008-076X 2008-0778 |
| language | English |
| last_indexed | 2024-03-07T23:54:57Z |
| publishDate | 2024-04-01 |
| publisher | Royan Institute (ACECR), Tehran |
| record_format | Article |
| series | International Journal of Fertility and Sterility |
| spelling | doaj.art-7423ff16463a429a8338f1529c33f4722024-02-18T08:39:20ZengRoyan Institute (ACECR), TehranInternational Journal of Fertility and Sterility2008-076X2008-07782024-04-0118211512210.22074/ijfs.2023.555601.1317705588Association of Follicular Fluid Antioxidants Activity with Aging and In Vitro Fertilization Outcome: A Cross-Sectional StudyMahsa Afrough0Roshan Nikbakht1Mahmoud Hashemitabar2Elham Ghalambaz3Sareh Amirzadeh4Adel Zardkaf5Sahar Adham6Maryam Mehdipour7Parvin Dorfeshan8Health Education Research Department, lnfertility Research and Treatment Center, ACECR, Ahvaz, IranFertility Infertility and Perinatology Research Center, Ahvaz Jundishapur University of Medical Science, Ahvaz, Iranlnfertility Research and Treatment Center, ACECR, Ahvaz, Iranlnfertility Research and Treatment Center, ACECR, Ahvaz, Iranlnfertility Research and Treatment Center, ACECR, Ahvaz, Iranlnfertility Research and Treatment Center, ACECR, Ahvaz, Iranlnfertility Research and Treatment Center, ACECR, Ahvaz, Iranlnfertility Research and Treatment Center, ACECR, Ahvaz, IranDepartment of Anatomical Science, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranBackground: This research was aimed at assessing the relationship between the follicular fluid (FF) antioxidantsactivity, aging and in vitro fertilization (IVF) outcome.Materials and Methods: The present cross-sectional study was carried out on 65 women undergoing IVF/intracytoplasmicsperm injection (IVF/ICSI) cycles due to unexplained infertility. Ovarian stimulation was performed using thelong gonadotropin-releasing hormone (GnRH) agonist protocol. After ovum pickup, FF was collected and processedto measure the level of superoxide dismutase (SOD), catalase (CAT) activity, total antioxidant capacity (TAC) andglutathione (GSH). Day 3 after ICSI, fresh embryos were transferred and later, possible pregnancy was assessed.Patients participating in this study were divided into four groups on the basis of age and pregnancy outcome.Results: SOD activity was not significantly different between the groups (P=0.218). GSH in the group whose participantswere aged ≤35 years and were pregnant was higher than that in other groups. CAT activity in groups withyounger participants was higher compared to the other groups. The mean TAC was higher in groups with pregnantparticipants compared to the non-pregnant women. Correlation analysis showed that: GSH level had a significantnegative correlation with age (P<0.001, R -0.55) and a significant positive correlation with pregnancy (P=0.015,R=0.30). CAT level also had a significant negative correlation with age (P<0.001, R=-0.42) and the level of TAC hada significant positive correlation with pregnancy (P<0.001, R=0.59).Conclusion: According to our results, the levels of TAC, GSH and CAT in younger and pregnant women were highercompared with those undergoing ICSI cycles. Given the correlation of FF antioxidant activity with age and pregnancy,it is necessary to carry out more research on these compounds and the maintenance of pregnancy.https://www.ijfs.ir/article_705588_caac522492efa99c44c7d35ad248f7bd.pdfagingcatalasefollicular fluid antioxidantsglutathioneinfertility |
| spellingShingle | Mahsa Afrough Roshan Nikbakht Mahmoud Hashemitabar Elham Ghalambaz Sareh Amirzadeh Adel Zardkaf Sahar Adham Maryam Mehdipour Parvin Dorfeshan Association of Follicular Fluid Antioxidants Activity with Aging and In Vitro Fertilization Outcome: A Cross-Sectional Study International Journal of Fertility and Sterility aging catalase follicular fluid antioxidants glutathione infertility |
| title | Association of Follicular Fluid Antioxidants Activity with Aging and In Vitro Fertilization Outcome: A Cross-Sectional Study |
| title_full | Association of Follicular Fluid Antioxidants Activity with Aging and In Vitro Fertilization Outcome: A Cross-Sectional Study |
| title_fullStr | Association of Follicular Fluid Antioxidants Activity with Aging and In Vitro Fertilization Outcome: A Cross-Sectional Study |
| title_full_unstemmed | Association of Follicular Fluid Antioxidants Activity with Aging and In Vitro Fertilization Outcome: A Cross-Sectional Study |
| title_short | Association of Follicular Fluid Antioxidants Activity with Aging and In Vitro Fertilization Outcome: A Cross-Sectional Study |
| title_sort | association of follicular fluid antioxidants activity with aging and in vitro fertilization outcome a cross sectional study |
| topic | aging catalase follicular fluid antioxidants glutathione infertility |
| url | https://www.ijfs.ir/article_705588_caac522492efa99c44c7d35ad248f7bd.pdf |
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